Zastosowanie technik biologii molekularnej do detekcji Erysiphe alphitoides (Griffon & Maubl.) U. Braun and S. Takam. w organach roślinnych

• Autorzy: Roszak R. , Baranowska M. , Belka M. , Behnke-Borowczyk J.

Warianty tytułu

EN

Applying the molecular biology techniques to the detection of Erysiphe alphitoides (Griffon & Maubl.) U. Braun and S. Takam. in plant parts

• Języki publikacji: PL

Abstrakty

EN

Erysiphe alphitoides causes the most common disease of assimilation apparatus of oaks of different age. It is believed that the pathogen overwinters in buds of the host plant or in the cracks of the bark. The aim of the study was to search for the presence of E. alphitoides in buds, leaves, and wood of sessile oak shoots using molecular techniques. Two hypotheses have been tested: (i) oaks are infected by E. alphitoides, and (ii) the pathogen overwinters in the host plant buds. The samples used in the study were collected form sessile oak trees (Miradz Forest District; 52°41'23.197"N, 18°25'33.942"E) in 2017, and consisted of dormant buds (collected in May), young leaves (collected in June), leaves with visible symptoms of the disease (collected in August), shoot with sip and surface layers of wood (collected in December). Additional part of buds was collected in May 2018. The PCR reaction was carried out with primers specific for E. alphitoides and E. hypophylla. For species identification Sanger method was used. The resulting sequences were compared using BLAST algorithm with reference sequences deposited in the NCBI database. Sequences from isolates obtained from leaves showed 97−99% similarity to the reference sequence of E. alphitoides. Pathogen did not occur in the superficial layers of shoots and buds. As some studies show, at very low temperatures (below –20°C), the mycelium of the pathogen dies, therefore further study should be undertaken on wintering of the pathogen causing the powdery mildew disease on oaks. It also should be examined whether the pathogen overwinters in the form of bagnial spores produced in chasmothecia overwintering on leaves, in the leaf buds, or in bark cracks, and whether the wintering site of the pathogen is related to the age of trees and or to the stands where the trees grow.

Słowa kluczowe

PL

lesnictwo; drzewa lesne; dab; Quercus; paki; liscie; pedy; maczniak prawdziwy debu; Erysiphe alphitoides; wykrywanie; techniki biologii molekularnej

• Wydawca: -
• Czasopismo: Sylwan
• Rocznik: 2019
• Tom: 163
• Numer: 09
• Opis fizyczny: s.740-745,tab.,bibliogr.

Twórcy

autor

Roszak R.

• Katedra Fitopatologii Leśnej, Uniwersytet Przyrodniczy w Poznaniu, ul.Wojska Polskiego 71C, 60-625 Poznań

autor

Baranowska M.

• Katedra Hodowli Lasu, Uniwersytet Przyrodniczy w Poznaniu, ul.Wojska Polskiego 71A, 60-625 Poznań

autor

Belka M.

• Katedra Fitopatologii Leśnej, Uniwersytet Przyrodniczy w Poznaniu, ul.Wojska Polskiego 71C, 60-625 Poznań

autor

Behnke-Borowczyk J.

• Katedra Fitopatologii Leśnej, Uniwersytet Przyrodniczy w Poznaniu, ul.Wojska Polskiego 71C, 60-625 Poznań

Bibliografia

• Baranowska-Wasilewska M., Behnke-Borowczyk J., Szewczyk W., Wajchman S. 2015. Mączniak prawdziwy dębu. Przegląd Leśniczy 3: 27.
• Behnke-Borowczyk J., Baranowska-Wasilewska M. 2017. Populacja mączniaka prawdziwego dębu w zachodniej Polsce. Acta Scientiarum Polonorum Silvarum Colendarum Ratio et Industria Lignaria 16 (4): 227-232. DOI: https://doi.org/10.17306/J.AFW.2017.4.23.
• Biuletyn monitoringu klimatu Polski, zima 2016/2017 (grudzień 2016 - luty 2017). 2017. Instytut Meteorologii i Gospodarki Wodnej – PIB.
• Braun U. 1995. The powdery mildews (Erysiphales) of Europe. Gustav Fischer, Jena.
• Cho S. E., Lee S. H., Lee S. K., Seo S. T., Shin H. D. 2017. Erysiphe alphitoides causes powdery mildew on Eucalyptus gunnii. Forest Pathology 48: e12377. DOI: https://doi.org/10.1111/efp.12377.
• Covey R. P. 1969. Effect of extreme cold on the overwintering of Podosphaera leucotricha. Plant Disease Report 53: 710.
• Desprez-Loustau M.-L., Feau N., Mougou-Hamdane A., Dutech C. 2011. Interspecific and intraspecific diversity in oak powdery mildews in Europe: coevolution history and adaptation to their hosts. Mycoscience 52: 163-175. DOI: https://doi.org/10.1007/S10267-010-0100-5.
• Feau N. A., Lauron-Moreau D., Piou B., Marçais C., Dutech C., Desprez-Loustau M.-L. 2012. Niche partitioning of the genetic lineages of the oak powdery mildew complex. Fungal Ecology 5: 154-162. DOI: https:// doi.org/ 10.1016/j.funeco.2011.12.003.
• Foex M. E. 1941. Invasion of European oaks by the powdery mildew (in French). Rev. Eaux Foręts 79: 338-349.
• Hajji M., Dreyer M., Marçais B. 2009. Impact of Erysiphe alphitoides on transpiration and photosynthesis in Quercus robur leaves. European Journal of Plant Pathology 125: 63-72. DOI: https://doi.org/10.1007/s10658-009-9458-7.
• Hamelin F., Bisson A., Desprez-Loustau M.-L., Fabre F., Mailleret L. 2016. Temporal niche differentiation of parasites sharing the same plant host: oak powdery mildew as a case study. Ecosphere, Ecological Society of America 7 (11): e01517. DOI: https://doi.org/10.1002/ecs2.1517.
• Kerling L. 1966. The hibernation of the oak mildew. Acta Bot Neerl 15: 76-83.
• Krótkoterminowa prognoza występowania ważniejszych szkodników i chorób infekcyjnych drzew leśnych w Polsce w 2017 roku. 2017. Instytut Badawczy Leśnictwa.
• Liyanage A. de S., Royle D. J. 1976. Overwintering of Sphaerotheca humuli, the cause of hop powdery mildew. Annals of Applied Biology 83: 381-394.
• Mańka K. 2005. Fitopatologia leśna. PWRiL, Warszawa.
• Marçais B., Desprez-Loustau M.-L. 2014. European oak powdery mildew: impact on trees, effects of environmental factors, and potential effects of climate change. Annals of Forest Science 71 (6): 633-642. DOI: https://doi.org/ 10.1007/s13595-012-0252-x.
• Marçais B., Kavkova M., Desprez-Loustau M.-L. 2009. Phenotypic variation in the phenology of ascospore production between European populations of oak powdery mildew. Annals of Forest Science 66: 814-822. DOI: https:// doi.org/10.1051/forest/2009077.
• Marçais B., Piou D., Dezette D., Desprez-Loustau M.-L. 2017. Can Oak Powdery Mildew Severity be Explained by Indirect Effects of Climate on the Composition of the Erysiphe Pathogenic. Ecology and Epidemiology Phytopathology 107 (5): 570-579. DOI: https://doi.org/10.1094/PHYTO-07-16-0268-R.
• Mougou A., Dutech C., Desprez-Loustau M.-L. 2008. New insights into the identity and origin of the causal agent of oak powdery mildew in Europe. Forest Pathology 38: 275-287. DOI: https://doi.org/10.1111/j.1439-0329.2008. 00544.x.
• Mougou-Hamdane A., Giresse X., Dutech C., Desprez-Loustau M.-L. 2010. Spatial distribution of lineages of oak powdery mildew fungi in France, using quick molecular detection methods. Annals of Forest Science 67: 21. DOI: https://doi.org/10.1051/forest/2009105.
• Mychayliv O., Sierota Z., Lech P. 2011. Wpływ warunków pogodowych na występowanie w młodnikach chorób apa-ratu asymilacyjnego. Komisja Technicznej Infrastruktury Wsi 6: 81-90.
• Phillips D. H., Burdekin D. 1982. Diseases of Forest and Ornamental Trees. Diseases of oak (Quercus spp.). Palgrave Macmillan, London.
• Przybył K. 2006. Lasy dębowe w Polsce. Dęby. Nasze drzewa leśne. Monografie popularnonaukowe 11: 752-756.
• Sierota Z., Szczepkowski A. 2014. Rozpoznawanie chorób infekcyjnych drzew leśnych. CILP, Warszawa.
• Spotts R. A., Chen P. M. 1984. Cold hardiness and temperature responses of healthy and mildew-infected terminal buds of apple during dormancy. Phytopathology 74: 542-544.
• Sucharzewska E. 2009. The development of Erysiphe alphitoides and E. hypophylla in the urban environment. Acta Mycologica 44 (1): 09-123.
• Szewczyk W., Kuźmiński R., Mańka M., Kwaśna H., Łakomy P., Baranowska-Wasilewska M., Behnke-Borowczyk J. 2015. Występowanie Erysiphe alphitoides w drzewostanach dębowych dotkniętych klęską powodzi. Leś. Pr. Bad. 76 (1): 73-77. DOI: https://doi.org/10.1515/frp-2015-0008.
• Tavanaei G. H. 2007. Study on biology of Microsphaera alphitoides coused powdery mildew on oak (Quercus petraea) in Arasbaran forests AGRIS.
• Wainhouse D., Daegan I. G., Denman S., Green S., Webber J. 2016. Agriculture and Forestry Climate Change Impacts Summary Report, Living with Environmental Change. DOI: https://doi.org/10.13140/RG.2.2.17378.73929.
• Woodward R. C., Waldie J. S. L., Steven H. M. 1929. Oak mildew and its control in forest nurseries. Forestry 3: 38-56.
• Yarwood C. E. 1957. Powdery mildews. Botanical Review 23: 235-300.

Identyfikatory

DOI

10.26202/sylwan.2019076