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2011 | 71 | S |
Tytuł artykułu

NMDA - induced electrophysiological activity in hippocampal formation slices - attempts to induce rhythmic oscillations

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Języki publikacji
EN
Abstrakty
EN
Theta rhythm is a model example of oscillation and synchrony in neural networks of the central nervous system. Cholinergic and GABAergic nature of the in vitro and in vivo induced theta rhythm has been earlier established. However, according to the recent report by Bland and colleagues (2007) it is suggested that glutamatergic septohippocampal projection represents a third, independent pathway capable of generating hippocampal field and cellular synchrony. With regard to above data, the aim of the study was to investigate whether the stimulation of NMDA receptors is able to induce rhythmic activity in the hippocampal formation maintained in complete de-afferentation. In the present work field potential and intracellular recordings were made from the CA1 and CA3 fields of hippocampal formation during the bath perfusion of the following concentration of NMDA: 1 µM, 3 µM, 10 µM, 30 µM, 50 µM and 80 µM. Application of all but 1 µM NMDA resulted in epileptic activity in aproximate range 0,1 - 1 Hz. The attempts of synchronization were observed in 23% and 31% of all active slices treated with 20 µM and 50 µM NMDA. Since an “effective dose” of NMDA which administration resulted in the highest probability to induce the attempts of synchronization was 50 µM NMDA, the influence of following mixtures on hippocampal EEG activity has been examined: 50 µM NMDA was tested with 10 µM 2-hydroxy-saclofen and 50 µM NMDA was tested with 10 µM bicuculine. Short attempts of synchronization of EEG activity were noticeable in 15% of active slices treated with the first mixture and in 21% of active slices treated with the other. The effect of NMDA was completely blocked after preincubation with 50 µM selective NMDA receptor antagonist D-AP5. I the light of above results it seems that stimulation of NMDA receptor itself is not sufficient to induce theta oscillations in completely deafferented hippocampal formation. Project supported by the European Union under European Social Fund (HUMAN - BEST INVESTMENT)
Słowa kluczowe
Wydawca
-
Rocznik
Tom
71
Numer
S
Opis fizyczny
p.43
Twórcy
  • Department of Neurobiology, University of Lodz, Lodz, Poland
autor
  • Department of Neurobiology, University of Lodz, Lodz, Poland
autor
  • Department of Neurobiology, University of Lodz, Lodz, Poland
  • Department of Neurobiology, University of Lodz, Lodz, Poland
autor
  • Department of Neurobiology, University of Lodz, Lodz, Poland
autor
  • Department of Neurobiology, University of Lodz, Lodz, Poland
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-1eca6b05-1dab-4e5d-8d8a-9484c1d8e106
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