In vitro regeneration of brahmi shoots using semisolid and liquid cultures and quantitative analysis of bacoside A

• Autorzy: Praveen N. , Naik P. M. , Manohar S. H. , Nayeem A. , Murthy H. N.
• Języki publikacji: EN

Abstrakty

EN

The major objectives of this study were to investigate an efficient rapid protocol for mass propagation of adventitious shoots of brahmi using semisolid and liquid cultures; and to assess the amount of bacoside A accumulated in the regenerated shoots. Leaf explants were grown in vitro on Murashige and Skoog semisolid medium supplemented with 0.5, 1.0, 1.5, 2.0 and 2.5 mg l⁻¹ 6-benzyladenine or kinetin (KN) or thidiazuron (TDZ) for 4 weeks. Adventitious shoots developed from leaf explants on all cytokinin supplemented media. After 4 weeks of incubation, leaf explants were split into two batches and one set was subcultured on semisolid medium and another set in liquid medium containing same concentration of cytokinins where they have come from. Highest rate of shoot regeneration was observed for explants cultured on medium with 2 mg l⁻¹ KN. The fresh and dry weight of shoots was also highest with this treatment. Liquid cultures were found suitable for proliferation of shoots (155.6 shoots per explant) and they also favored highest biomass accumulation (8.60 g fresh and 0.35 g dry biomass). The bacoside A contents were determined in shoots using HPLC. Analysis revealed that, the contents were highest with shoots regenerated on medium supplemented with 2 mg l⁻¹ KN. The amount of bacoside A was highest in the shoots regenerated in liquid medium (11.92 mg g⁻¹ DW) and it was 2.2-fold higher compared to shoots grown on semisolid cultures.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 4
• Opis fizyczny: p.723-728,fig.,ref.

Twórcy

autor

Praveen N.

• Department of Botany, Karnatak University, Dharwad 580 003, India

autor

Naik P. M.

• Department of Botany, Karnatak University, Dharwad 580 003, India

autor

Manohar S. H.

• Department of Botany, Karnatak University, Dharwad 580 003, India

autor

Nayeem A.

• Department of Botany, Karnatak University, Dharwad 580 003, India

autor

Murthy H. N.

• Department of Botany, Karnatak University, Dharwad 580 003, India

Bibliografia

• Adelberg J (2004) Plant growth and sugar utilization in an agitated, thin-film liquid system for micropropagation. In Vitro Cell Dev Biol Plant 40:245–250. doi:10.1079/IVP2003511
• Anbarasi K, Vani G, Balakrishna K, Shyamala Devi CS (2005) Creatine kinase isoenzyme patterns upon chronic exposure to cigarette smoke: protective effect of bacoside A. Vascul Pharmacol 42:57–61
• Bhattacharya SK, Kumar A, Ghosal S (1999) Effect of Bacopa monniera on animal models of Alzheimer’s disease and perturbed central cholinergic markers of cognition in rats. Res Commun Pharmacol Toxicol 4:111–112
• Bhattacharya SK, Bhattacharya A, Kumar A, Ghosal S (2000) Antioxidant activity of Bacopa monniera in rat frontal cortex, striatum and hippocampus. Phytother Res 14:174–179. doi: 10.1002/ SICI)1099-1573(200005)14:3<174::AID-PTR624>3.0.CO;2-O
• DiCosmo F, Towers GHN (1984) Stress and secondary metabolism in cultured plant cells. In: Timmerman BN, Steelink FA, Loewus FA (eds) Recent advances in phytochemistry, vol 18. Plenum, New York, pp 97–175
• Ilan A, Ziv M, Halvey AH (1995) Propagation and corm development of Brodiaea in liquid cultures. Sci Hortic (Amsterdam) 63:101–112. doi:10.1016/0304-4238(95)00785-R
• Jo UA, Murthy HN, Hahn EJ, Paek KY (2008) Micropropagation of Alocasia amazoninca using semisolid and liquid cultures. In Vitro Cell Dev Biol Plant 44:26–32. doi:10.1007/s11627-007-9081-2
• Kim EK, Hahn EJ, Murthy HN, Paek KY (2004) Enhanced shoot and bulblet proliferation of garlic (Allium sativum L.) in bioreactor system. J Hortic Sci Biotechnol 79:818–822
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473–497. doi:10.1111/j.1399-3054.1962.tb08052.x
• Murthy PBS, Raju VR, Ramakrishna T, Chakravarthy MS, Kumar KV, Kannababu S, Subbaraju GV (2006) Estimation of twelve bacopa saponins in Bacopa monnieri extracts and formulations by high-performance liquid chromatography. Chem Pharm Bull (Tokyo) 54:907–911. doi:10.1248/cpb.54.907
• Nadkarni KM (1976) Indian Materia Medica. Popular Prakashan, Bombay, pp 624–625
• Park SY, Murthy HN, Paek KY (2000) Mass multiplication of protocorm-like bodies (PLBs) using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell Tissue Organ Cult 63:67–72. doi:10.1023/A:1006420116883
• Pravina K, Ravindra KR, Goudar KS, Vinod DR, Joshua AJ, Wasim P, Venkateswarlu K, Saxena VS, Amit A (2007) Safety evaluation of BacoMindTM in healthy volunteers: a phase I study. Phytomedicine 14:301–308. doi:10.1016/j.phymed.2007. 03.010
• Rao SR, Ravishankar GA (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv 20:101–153. doi:10.1016/S0734-9750(02)00007-1
• Russo A, Borrelli F (2005) Bacopa monniera, a reputed nootropic plant: an overview. Phytomedicine 12:305–317. doi:10.1016/j.phymed.2003.12.008
• Seitz HU, Hinderer W (1988) Anthocyanins. In: Constabel F, Vasil I (eds) Cell culture and somatic cell genetics of plants, vol 5. Academic, San Diego, pp 49–76
• Shrivastava N, Rajani M (1999) Multiple shoot regeneration and tissue culture studies Bacopa monnieri (L.) Pennell. Plant Cell Rep 18:919–923. doi:10.1007/s002990050684
• Singh HK, Dhawan BN (1997) Neurophychopharmacological effects of the Ayurvedic nootropic Bacopa monniera Linn. (Brahmi). Indian J Pharmacol 29:8359–8365
• Tiwari V, Singh BD, Tiwari KN (1998) Shoot regeneration and somatic embryogenesis from different explants of Brahmi (Bacopa monniera (L.) Wettst.). Plant Cell Rep 17:538–543. doi: 10.1007/s002990050438
• Tiwari V, Tiwari KN, Singh BD (2001) Comparative studies of cytokinins on in vitro propagation of Bacopa monniera. Plant Cell Tissue Organ Cult 66:9–16. doi:10.1023/A:1010652006417

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