The activity of plasma membrane hyperpolarization-activated Ca2plus channels during pollen development of Pyrus pyrifolia

• Autorzy: Wu J. , Jin C. , Qu H. , Jiang X. , Wu J. , Xu G. , Zhang S.
• Języki publikacji: EN

Abstrakty

EN

Calcium (Ca²⁺) plays crucial roles in regulation of pollen tube growth. The influx of Ca²⁺ into the pollen tube is mediated by ion channels, and the density and activity of Ca²⁺ channels in pollen plasma membranes critically determines their electrical properties. In this report, using whole-cell and single-channel patch-clamping techniques, we investigated developmental changes of hyperpolarization-activated Ca²⁺ channel activity in pear (Pyrus pyrifolia) pollen and its relationship with pollen viability. For both pollen and pollen tubes, hyperpolarization-activated Ca²⁺ channels had the same conductance and cAMP sensitivity, indicating that they were the same channels. However, the Ca²⁺ current density in pollen tube protoplasts was greater than that in pollen protoplasts. Compared with day-3 flowers’ pollen, hyperpolarizationactivated Ca²⁺ current density was significantly lower in day 0 and day 3 flowers’ pollen, which was consistent with the pollen germination and pollen tube growth, indicating that pollen protoplasts’ increased Ca²⁺ current density may have enhanced the pollen viability. During pollen tube elongation, pollen tube plasma membrane Ca²⁺ current density increased with increased length pollen tubes up to 300 µm. All of these results indicated that hyperpolarization-activated Ca²⁺ channel activity was associated with in pear pollen development and may have a causal link between Ca²⁺ channel activity and pollen viability.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 3
• Opis fizyczny: p.969-975,fig.,ref.

Twórcy

autor

Wu J.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, China

autor

Jin C.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, China

autor

Qu H.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, China

autor

Jiang X.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, China

autor

Wu J.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, China

autor

Xu G.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, China

autor

Zhang S.

• State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, 210095 Nanjing, China

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