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2009 | 69 | 3 |
Tytuł artykułu

The role of low molecular weight thiols in thimerosal toxicity - an in votro study

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Thimerosal (TH), an ethylmercury complex of thiosalicylic acid has been used as preservative in vaccines. Inspired by a known high affi nity of mercury for thiol groups, we examined whether the presence of L-cysteine (Cys), D,L-homocysteine (Hcy), Nacetyl cysteine (NAC), L-methionine (Met) and glutathione (GSH) in extracellular space could infl uence the viability, intracellular calcium concentration ([Ca2+]i ) and mitochondrial membrane potential in rat cerebellar granule cells. The cells were exposed to 500 nM TH for 48 h or 15 μM TH for 10 min. The loss of cells viability could be prevented partially or wholly, in a dose-dependent manner, by 60, 120 or 600 μM Cys, Hcy, NAC and GSH, but not by Met. The elevation in [Ca2+]i and mitochondrial potential induced by 25 μM TH were abolished by all compounds studied, except for Met, at 600 μM. The loss of the ethylmercury moiety from TH as a result of interaction with thiols studied was monitored by 1 Hand 199Hg-NMR spectroscopy. This extracelullar process may be responsible for the neuroprotection seen in cerebellar cell culture, but also provides a molecular pathway for redistribution of TH derived toxic ethylmercury in the organism.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
69
Numer
3
Opis fizyczny
p.316
Twórcy
autor
  • Department of Neurochemistry, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
  • Laboratory of Biological NMR, Warsaw, Poland
autor
  • Laboratory of Biological NMR, Warsaw, Poland
autor
  • Department of Neurochemistry, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
autor
  • Department of Biophysics, Institute of Biochemistry and Biophysics, Warsaw, Poland
  • Department of Neurochemistry, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
Bibliografia
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-1465cb85-5ec3-4a4a-a56a-cc131a9cf3fa
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