Impact of low and high fluence rates of UV-B radiation on growth and oxidative stress in Phormidium foveolarum and Nostoc muscorum under copper toxicity: differential display of antioxidants system

• Autorzy: Singh V.P. , Srivastava P. K. , Prasad S. M.
• Języki publikacji: EN

Abstrakty

EN

In the present study, impact of low (UV-BL) and high (UV-BH) fluence rates of UV-B on growth, oxidative stress and antioxidant system was studied in two cyanobacteria i.e. Phormidium foveolarum and Nostoc muscorum under Cu (2 and 5 µM) toxicity after 24 and 72 h of experiments. UV-BH and Cu treatment decreased growth of both the cyanobacteria and Cu induced decrease in growth was accompanied by a significant increase in Cu accumulation. Levels of reactive oxygen species (ROS), i.e. superoxide radicals (SOR; O₂⁻ ) and hydrogen peroxide (H₂O₂) were significantly increased by Cu and UV-BH exposure which in turn accelerated lipid peroxidation (malondialdehyde: MDA) and protein oxidation (reactive carbonyl groups: RCG). Activities of enzymatic antioxidants, such as superoxide dismutase (SOD), peroxidase (POD) and glutathione-S-transferase (GST) were increased by both doses of Cu as well as UV-B. Conversely, Cu and UV-BH drastically decreased catalase (CAT) activity. After the commencement of 24 h of treatment with Cu alone and together with UV-BH, non-protein thiols (NP-SH) contents were decreased while after 72 h, a reverse trend was noticed. Unlike NP-SH, cysteine content decreased appreciably during the treatments. In contrast to this, low dose (UV-BL) of UV-B did not influence growth, SOR, H₂O₂, MDA and RCG contents. An improvement in CAT activity and NP-SH content was observed under Cu and UV-BL treatment; hence, UV-BL treatment resulted into certain degree of protection against Cu toxicity in both the organisms. Thus, the results showed that UV-BH and UVBL exerted differential effects on both the organisms under Cu toxicity, and compared to N. muscorum, P. foveolarum was less affected by Cu and UV-BH.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 6
• Opis fizyczny: p.2225-2239,fig.,ref.

Twórcy

autor

Singh V.P.

• Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, 211002 Allahabad, India

autor

Srivastava P. K.

• Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, 211002 Allahabad, India

autor

Prasad S. M.

• Ranjan Plant Physiology and Biochemistry Laboratory, Department of Botany, University of Allahabad, 211002 Allahabad, India

Bibliografia

• Aebi II (1984) Catalase in vitro. Methods Enzymol 105:121–126
• Andrade LR, Farina M, Amado Filho GM (2004) Effects of copper on Enteromorpha flexuosa (Chlorophyta) in vitro. Ecotoxicol Environ Saf 58:117–125
• Briat JF, Lebrun M (1999) Plant responses to metal toxicity. CR Acad Sci Paris 322:43–54
• Brown BA, Jenkins GI (2008) UV-B signaling pathways with different fluence-rate response profiles are distinguished in mature Arabidopsis leaf tissue by requirement for UVR8, HY5, and HYH. Plant Physiol 146:576–588
• Brown MT, Newman JE (2003) Physiological responses of Gracilariopsis longissima (S.G. Gmelin) Steentoft, L.M. Irvine and Farnham (Rhodophyceae) to sublethal copper concentrations. Aquat Toxicol 64:201–213
• Brown BA, Cloix C, Jiang GH, Kaiserli E, Herzyk P, Kliebenstein DJ, Jenkins GI (2005) A UV-B-specific signaling component orchestrates plant UV protection. Proc Natl Acad Sci USA 102:18225–18230
• Brun LA, Maillet J, Hinsiger P, Pépin N (2001) Evaluation of copper availability to plants in copper-contaminated vineyard soils. Environ Pollut 111:293–302
• Christie JM, Jenkins GI (1996) Distinct UV-B and UV-A blue light signal transduction pathways induce chalcone synthase gene expression in Arabidopsis cells. Plant Cell 8:1555–1567
• Dat J, Vandenabeele S, Vranovaá E, Van Montagu M, Inzé D, Van Breusegem F (2000) Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci 57:779–795
• De Vos CHR, Schat H, De Waal MAM, Vooijs R, Ernst WHO (1991) Increased resistance to copper-induced damage of the root cell plasmalemma in copper tolerant Silene cucubalus. Physiol Plant 82:523–528
• Dominguez-Solis J, Guttierez-Alcala G, Romero L, Gotor C (2001) The cytosolic O-acetylserine(thiol)lyase gene is regulated by heavy metals and can function in cadmium tolerance. J Biol Chem 276:9297–9302
• Edwards R, Dixon DD (2004) Metabolism of natural and xenobiotics substrates by the plant glutathione-S-transferase superfamily. In: Sandermann H (ed) Molecular ecotoxicology of plants, ecological studies, vol 170. Spriger, Berlin, pp 17–50
• Ellmann GL (1959) Tissue sulfhydryl groups. Arch Biochem Biophys 82:70–77
• Elstner EF, Heupel A (1976) Inhibition of nitrite formation from hydroxyl ammonium chloride: a simple assay for superoxide dismutase. Anal Biochem 70:616–620
• Frohnmeyer H, Staiger D (2003) Ultraviolet-B radiation-mediated responses in plants. Balancing damage and protection. Plant Physiol 133:1420–1428
• Gahagen HE, Halm RE, Abeles FB (1968) Effect of ethylene on peroxidase activity. Physiol Plant 21:1270–1279
• Gaitonde MK (1967) A spectrophotometric method for the direct determination of cysteine in the presence of other naturally occurring amino acids. Biochem J 104:627–633
• Gajewska E, SklodowskaM(2010) Differential effect of equal copper, cadmium and nickel concentration on biochemical reactions in wheat seedlings. Ecotoxicol Environ Saf 73:996–1003
• Gao K, Yu H, Brown MT (2007) Solar PAR and UV radiation affects the physiology and morphology of the cyanobacterium Anabaena sp. PCC 7120. J Photochem Photobiol, B 89:117–124
• Giannopolitis CN, Reis SK (1977) Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol 59:309–314
• Habig WH, Pabst MJ, Jakoby WB (1974) Glutathione-S-transferases, the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130–7139
• Häder DP, Kumar HD, Smith RC, Worrest RC (2007) Effects of solar UV radiation on aquatic ecosystems and interactions with climate change. Photochem Photobiol Sci 6:267–285
• Han T, Kang SH, Park JS, Lee HK, Brown MT (2008) Physiological responses of Ulva pertusa and U. armoricana to copper exposure. Aquat Toxicol 86:176–184
• He YY, Häder DP (2002) UV-B-induced formation of reactive oxygen species and oxidative damage of the cyanobacterium Anabaena sp.: protective effects of ascorbic acid and N-acetyl-Lcysteine. J Photochem Photobiol, B 66:115–124
• Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
• Hong Y, Hu HY, Xie X, Sakoda A, Sagehashi M, Li FM (2009) Gramine-induced growth inhibition, oxidative damage and antioxidant responses in freshwater cyanobacterium Microcystis aeruginosa. Aquat Toxicol 91:262–269
• Hurtubise RD, Havel JE (1998) The effects of ultraviolet-B radiation on freshwater invertebrates: experiments with a solar simulator. Limnol Oceanogr 43:1082–1088
• Kanoun-Boulé M, Vicente JAF, Nabais C, Prasad MNV, Freitas H (2009) Ecophysiological tolerance of duckweeds to copper. Aquat Toxicol 91:1–9
• Karentz S, Cleaver JE, Mitchell DL (1991) DNA damage in the Antarctic. Nature 350:28
• Kim BC, Tennessen DJ, Last RL (1998) UV-B-induced photomorphogenesis in Arabidopsis thaliana. Plant J 15:667–674
• Levine RL, Williams JA, Stadtman ER, Shacter E (1994) Carbonyl assay for determination of oxidatively modified proteins. Methods Enzymol 233:346–357
• Lohscheider JN, Strittmatter M, Kupper H, Adamska I (2011) Vertical distribution of epibenthic freshwater cyanobacterial Synechococcus spp. strains depends on their ability for photoprotection. PLoS ONE 6(5):e20134. doi:10.1371/journal. pone.0020134
• Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
• Luna CM, Gonzalez CA, Trippi VS (1994) Oxidative damage caused by an excess of copper in oat leaves. Plant, Cell Environ 35:11–15
• Marrs KA (1996) The function and regulation of glutathione-Stransferases in plants. Annu Rev Plant Physiol Plant Mol Biol 47:127–158
• Mazza CA, Zavala J, Scopel AL, Ballaré CL (1999) Perception of solar UVB radiation by phytophagous insects: behavioral responses and ecosystem implications. Proc Natl Acad Sci USA 96:980–985
• Mendoza-Cozatl D, Loza-Tavera H, Hernandez-Navarro A, Moreno-Sanchez R (2005) Sulfur assimilation and glutathione metabolism under cadmium stress in yeast, protists and plants. FEMS Microbiol Rev 29:653–671
• Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
• Mohammed AR, Tarpley L (2011) Morphological and physiological responses of nine southern US rice cultivars differing in their tolerance to enhanced ultraviolet-B radiation. Environ Exp Bot 70:174–184
• Morelli E, Scarano G (2004) Copper-induced changes of non-protein thiols and antioxidant enzymes in the marine microalga Phaeodactylum tricornutum. Plant Sci 167:289–296
• Morris DP, Zagarese H, Williamson CE, Balserio EG, Hargreaves BR, Modenutti B, Moeller R, Queimalinos C (1995) The attenuation of solar UV radiation in lakes and the role of dissolved organic carbon. Limnol Oceanogr 40:1381–1391
• Postius C, Kenter U, Wacker A, Ernst A, Böger P (1998) Light causes selection among two phycoerythrin-rich Synechococcus isolates from Lake Constance. FEMS Microbiol Ecol 25:171–178
• Rai LC, Tyagi B, Mallick N, Rai PK (1995) Interactive effects of UVB and copper on photosynthetic activity of the cyanobacterium Anabaena doliolum. Environ Exp Bot 35:177–185
• Rai LC, Tyagi B, Rai PK, Mallick N (1998) Interactive effects of UVB and heavy metals (Cu and Pb) on nitrogen and phosphorus metabolism of a N₂-fixing cyanobacterium Anabaena doliolum. Environ Exp Bot 39:221–231
• Shahbaz M, Tseng MH, Stuiver CEE, Koralewska A, Posthumus FS, Venema JH, Parmar S, Schat H, Hawkesford MJ, De Kok LJ (2010) Copper exposure interferes with the regulation of the uptake, distribution and metabolism of sulfate in Chinese cabbage. J Plant Physiol 167:438–446
• Singh SP, Häder DP, Sinha RP (2010) Cyanobacteria and ultraviolet radiation (UVR) stress: mitigation strategies. Age Res Rev 9:79–90
• Sinha RP, Häder DP (2000) Effects of UV-B radiation on cyanobacteria. Recent Res Dev Photochem Photobiol 4:239–246
• Smith RC, Baker KS (1979) Penetration of UV-B and biologically effective dose-rates in natural water. Photochem Photobiol 50:311–323
• Stoiber TL, Shafer MM, Karner-Perkins DA, Hemming JDC, Armstrong DE (2007) Analysis of glutathione endpoint for measuring copper stress in Chlamydomonas reinhardtii. Environ Toxicol Chem 26:1563–1571
• Tripathi BN, Gaur JP (2004) Relationship between copper-and zincinduced oxidative stress and proline accumulation in Scenedesmus sp. Planta 219:397–404
• Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant system in acid rain-treated bean plants. Plant Sci 151:59–66
• Wang G, Chen K, Chen L, Hu C, Zhang D, Liu Y (2008) The involvement of the antioxidant system in protection of desert cyanobacterium Nostoc sp. against UV-B radiation and the effects of exogenous antioxidants. Ecotoxicol Environ Saf 69:150–157
• Wang QF, Hou YH, Miao JL, Li GY (2009) Effect of UV-B radiation on the growth and antioxidant enzymes of Antarctic sea ice microalgae Chlamydomonas sp ICE-L. Acta Physiol Plant 31: 1097–1102
• Wirstam M, Blomberg MRA, Siebahn PEM (1999) Reaction mechanism of compound I formation in heme peroxidases: a density functional theory study. J Am Chem Soc 121:10178–10185
• Yruela I (2005) Copper in plants. Braz J Plant Physiol 17:145–156
• Zavala JA, Scopel AL, Ballaré CL (2001) Effects of ambient UV-B radiation on soybean crops: Impact on leaf herbivory by Anticarsia gemmatalis. Plant Ecol 00:1–10
• Zhu YL, Pilon-Smits EAH, Tarun AS, Weber SU, Jouanin L, Terry N (1999) Cadmium tolerance and accumulation in Indian mustard is enhanced by overexpressing c-glutamylcysteine synthetase. Plant Physiol 121:1169–1177

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