Development of an optimal culture system for callogenesis of Chrysanthemum indicum protoplasts

• Autorzy: Eeckhaut T. , Huylenbroeck J. V.
• Języki publikacji: EN

Abstrakty

EN

This study is a comparison of four methods to induce calli formation in a protoplast culture of Chrysanthemum indicum. Culture in liquid medium (17.6 calli/10⁵ protoplasts) was preferable to culture in solid agarose beads, although the efficiency of the latter could be improved by layering them on glass beads (12.5 vs. 0.83 calli/10⁵ protoplasts). Culture of protoplasts on moistened filter paper was unsuccessful. In the liquid media, microcalli and calli were induced efficiently and easily after 6 weeks. These effects may be explained by reduced toxicity due to cell breakdown and improved aeration.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 4
• Opis fizyczny: p.1547-1551,fig.,ref.

Twórcy

autor

Eeckhaut T.

• Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit-Applied Genetics and Breeding, Caritasstraat 21, 9090, Melle, Belgium

autor

Huylenbroeck J. V.

• Institute for Agricultural and Fisheries Research (ILVO), Plant Sciences Unit-Applied Genetics and Breeding, Caritasstraat 21, 9090, Melle, Belgium

Bibliografia

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Uwagi

PL

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