Essential genes of the macrophage response to Staphylococcus aureus exposure

• Autorzy: Sun A. , Zhang H. , Pang F. , Niu G. , Chen J. , Zhang J.
• Języki publikacji: EN

Abstrakty

EN

Background: Although significant advances have been made in understanding the mechanisms of macrophage response to Staphylococcus aureus infection, the molecular details are still elusive. Identification of the essential genes and biological processes of macrophages that are specifically changed at different durations of S. aureus exposure is of great clinical significance. Methods: We aimed to identify the significantly changed genes and biological processes of S. aureus-exposed macrophages. We systematically analyzed the macrophage gene expression profile GSE 13670 database with 8 h, 24 h or 48 h S. aureus infection. The results were further confirmed by western blot and quantitative polymerase chain reaction (qPCR) analyses. Results: After 8 h of S. aureus infection, the expression of 624 genes was significantly changed. Six hundred thirteen differentially expressed genes (DEGs) were identified after 24 h of S. aureus infection. Two hundred fifty-three genes were significantly changed after 48 h of S. aureus infection. STAT1 was consistently up-regulated in these three treatments. TP53, JAK2, CEBPA, STAT3, MYC, CTNNB1 and PRKCA were only identified in the 8 h or 24 h S. aureus infection groups. CTNNB1 and PRKCA were for the first time identified as potential essential genes in S. aureus infection of macrophages. In the Gene Ontology (GO) term analysis, the defense response was shown to be the most significantly changed biological process among all processes; KEGG pathway analysis identified the JAK-STAT signaling pathway involved in early infection. Conclusions: Our systematic analysis identified unique gene expression profiles and specifically changed biological processes of the macrophage response to different S. aureus exposure times.

• Wydawca: -
• Czasopismo: Cellular and Molecular Biology Letters
• Rocznik: 2018
• Tom: 23
• Opis fizyczny: p.1-14,fig.,ref.

Twórcy

autor

Sun A.

• Department of Clinical Laboratory, Liaocheng People’s Hospital, 67 West Dongchang Road, Liaocheng 252000, Shandong Province, People’s Republic of China

autor

Zhang H.

• Department of Clinical Laboratory, Liaocheng People’s Hospital, 67 West Dongchang Road, Liaocheng 252000, Shandong Province, People’s Republic of China

autor

Pang F.

• Department of Clinical Laboratory, Liaocheng People’s Hospital, 67 West Dongchang Road, Liaocheng 252000, Shandong Province, People’s Republic of China

autor

Niu G.

• Department of Clinical Laboratory, Liaocheng People’s Hospital, 67 West Dongchang Road, Liaocheng 252000, Shandong Province, People’s Republic of China

autor

Chen J.

• Department of Clinical Laboratory, Liaocheng People’s Hospital, 67 West Dongchang Road, Liaocheng 252000, Shandong Province, People’s Republic of China

autor

Zhang J.

• Department of Clinical Laboratory, Liaocheng People’s Hospital, 67 West Dongchang Road, Liaocheng 252000, Shandong Province, People’s Republic of China

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Identyfikatory

DOI

10.1186/s11658-018-0090-4