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2013 | 35 | 02 |

Tytuł artykułu

Morphological markers to correlate bud and anther development with microsporogenesis and microgametogenesis in pepper (Capsicum annuum L.)

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The identification of microspores or pollen grains at particular developmental stages during microsporogenesis or microgamentogenesis is an important step for different basic and applied purposes. Among them, the most relevant example from a biotechnological perspective is the production of androgenic doubled haploids. For this and other techniques, precise, fast, easy and reliable criteria to identify flower buds carrying microspores or pollen at particular stages are essential. In anthocyanin-producing pepper types, the particularities of flower development allow for the identification of several morphological markers potentially useful as criteria for such an identification. In this work, our aim was to determine the easiest and more accurate criterion to correlate visible, measurable traits of bud and anther development with each of the individual stages of microsporogenesis and microgametogenesis. For this, we used three Spanish sweet pepper F1 hybrids (‘Herminio’, ‘Gacela’ and ‘A´ guila’). We analyzed and discussed the accuracy and practical usefulness of using anther length, bud length, anther purple pigmentation and the ratio between calyx length and bud length (calyx/ bud ratio) as predictors of individual microspore/pollen developmental stages. According to our results, we propose a combination of calyx/bud ratio and anther pigmentation as an easy, fast and accurate criterion potentially applicable to anthocyanin-producing pepper cultivars to determine their particular markers.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

02

Opis fizyczny

p.627-633,fig.,ref.

Twórcy

  • COMAV, Universitat Polite`cnica de Vale`ncia, CPI, Edificio 8E-Escalera I, Camino de Vera, S/N, 46022 Valencia, Spain
  • COMAV, Universitat Polite`cnica de Vale`ncia, CPI, Edificio 8E-Escalera I, Camino de Vera, S/N, 46022 Valencia, Spain
  • COMAV, Universitat Polite`cnica de Vale`ncia, CPI, Edificio 8E-Escalera I, Camino de Vera, S/N, 46022 Valencia, Spain

Bibliografia

  • Barany I, Gonzalez-Melendi P, Fado´n B, Mityko J, Risuen˜o MC, Testillano PS (2005) Microspore-derived embryogenesis in pepper (Capsicum annuum L.): subcellular rearrangements through development. Biol Cell 97:709–722
  • Barany I, Fadon B, Risueno MC, Testillano PS (2010) Cell wall components and pectin esterification levels as markers of proliferation and differentiation events during pollen development and pollen embryogenesis in Capsicum annuum L. J Exp Bot 61:1159–1175. doi:10.1093/jxb/erp392
  • Buyukalaca S, Comlekcioglu N, Abak K, Ekbic E, Kilic N (2004) Effects of silver nitrate and donor plant growing conditions on production of pepper (Capsicum annuum L.) haploid embryos via anther culture. Eur J Hortic Sci 69(5):206–209
  • Dumas de Vaulx R, Chambonnet D, Pochard E (1981) Culture in vitro d’anthe`res de piment (Capsicum annuum L.): ame`lioration des taux d’obtenction de plantes chez diffe´rents ge´notypes par des traitments a` ?35 C. Agronomie 1 (10):859-864
  • Dunwell JM (2010) Haploids in flowering plants: origins and exploitation. Plant Biotechnol J 8(4):377–424. doi:10.1111/j.1467-7652.2009.00498.x
  • Ercan N, Sensoy FA, Sirri Sensoy A (2006) Influence of growing season and donor plant age on anther culture response of some pepper cultivars (Capsicum annuum L.). Sci Hort 110(1):16–20
  • Irikova T, Grozeva S, Rodeva V (2011) Anther culture in pepper (Capsicum annuum L.) in vitro. Acta Physiol Plant 33(5):1559–1570. doi:10.1007/s11738-011-0736-6
  • Kim M, Kim J, Yoon M, Choi DI, Lee KM (2004) Origin of multicellular pollen and pollen embryos in cultured anthers of pepper (Capsicum annuum). Plant Cell, Tissue Organ Cult 77:63–72
  • Kim M, Jang IC, Kim JA, Park EJ, Yoon M, Lee Y (2008) Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture. Plant Cell Rep 27(3):425–434
  • Koleva-Gudeva LR, Spasenoski M, Trajkova F (2007) Somatic embryogenesis in pepper anther culture: the effect of incubation treatments and different media. Sci Hort 111(2):114–119
  • Lantos C, Juha´sz A, Somogyi G, O ¨ tvo¨s K, Va´gi P, Miha´ly R, Kristo´f Z, Somogyi N, Pauk J (2009) Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat. Plant Cell Tiss Org Cult 97(3):285–293. doi:10.1007/s11240-009-9527-9
  • Ltifi A, Wenzel G (1994) Anther culture of hot and sweet pepper (Capsicum annuum L.): influence of genotype and plant growth temperature. Capsicum Eggplant Newsl 13:74–77
  • Mityko J, Andrasfalvy A, Csillery G, Fari M (1995) Anther culture response in different genotypes and F1 hybrids of pepper (Capsicum Annuum L). Plant Breed 114(1):78–80
  • Nowaczyk P, Kisiala A (2006) Effect of selected factors on the effectiveness of Capsicum annuum L. anther culture. J Appl Genet 47(2):113–117
  • Regner F (1996) Anther and microspore culture in Capsicum. In: Jain SM, Sopory SK, Veilleux RE (eds) In vitro haploid production in higher plants, vol 3. Kluwer, Dordrecht, pp 77–89
  • Regnet F (1994) Microspore culture of Capsicum annuum. Capsicum Eggplant Newsl 13(1114):69–70
  • Salas P, Rivas-Sendra A, Prohens J, Seguı´-Simarro JM (2012) Influence of the stage for anther excision and heterostyly in embryogenesis induction from eggplant anther cultures. Euphytica 184(2):235–250. doi:10.1007/s10681-011-0569-9
  • Seguı´-Simarro JM (2010) Androgenesis revisited. Bot Rev 76(3): 377–404. doi:10.1007/s12229-010-9056-6
  • Seguı´-Simarro JM, Nuez F (2005) Meiotic metaphase I to telophase II is the most responsive stage of microspore development for induction of androgenesis in tomato (Solanum lycopersicum). Acta Physiol Plant 27(4B):675–685
  • Seguı´-Simarro JM, Corral-Martı´nez P, Parra-Vega V, Gonza´lez-Garcı´a B (2011) Androgenesis in recalcitrant solanaceous crops. Plant Cell Rep 30(5):765–778. doi:10.1007/s00299-010-0984-8
  • Shivanna KR (2003) Pollen biology and biotechnology. Science Publishers Inc., Enfield
  • Supena EDJ, Muswita W, Suharsono S, Custers JBM (2006a) Evaluation of crucial factors for implementing shed-microspore culture of Indonesian hot pepper (Capsicum annuum L.) cultivars. Sci Hort 107(3):226–232
  • Supena EDJ, Suharsono S, Jacobsen E, Custers JBM (2006b) Successful development of a shed-microspore culture protocol for doubled haploid production in Indonesian hot pepper (Capsicum annuum L.). Plant Cell Rep 25(1):1–10

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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