Simultaneous determination of multi – component mycotoxin in feeds by liquid chromatography – tandem mass spectrometry

• Autorzy: Pietruszka K. , Sell B. , Burek O. , Wisniewska-Dmytrow H.
• Języki publikacji: EN

Abstrakty

EN

A multiresidue method for determination and quantification of Fusarium mycotoxins: deoxynivalenol, zearalenone, T-2 toxin, HT-2 toxin, and metabolite of Aspergillus and Penicillium species - ochratoxin A in feeds was described. The method was based on the simultaneous extraction of selected mycotoxins from matrix, followed by liquid chromatography coupled with tandem mass spectrometry using a hybrid triple quadmpole - linear ion trap mass spectrometer with the multiple reaction monitoring in both positive- and negative-ion modes. The method was validated in accordance with the Commision Decision 2002/657/EC requirements. The mean recoveries of mycotoxins from spiked feed samples ranged from 74.6% to 113.9%, whereas limit of detection and quantification ranged from 0.72 to 12.4 µg/kg and 1.86 to 31.7 µg/kg, respectively.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 4
• Opis fizyczny: p.567-572,fig.,ref.

Twórcy

autor

Pietruszka K.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Sell B.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Burek O.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Wisniewska-Dmytrow H.

• Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Berthiller F., Sulyok M., Krska R., Schuhmacher R.: Chromatographic methods for the simultaneous determination of mycotoxins and their conjugates in cereals. Int J Food Microbiol 119, 33-37.
• 2. Chełkowski J.: Mikotoksyny i grzyby toksynotwórcze, jako istotny wskaźnik jakości żywności i pasz. Polskie Drobiarstwo 10, 22-26.
• 3. Dworecka-Kaszak B.: Mikologia weterynaryjna. Edited by SGGW, Warszawa, 2008.
• 4. European Commission Regulation No. 576/2006. Off J Eur Commun L 229, 7-9.
• 5. European Commission Regulation No. 1881/2006. Off J Eur Commun L 364, 15-17.
• 6. European Commission Regulation No. 401/2006. Off J Eur Commun L 70, 12-34.
• 7. European Commission Regulation No. 152/2009. Off J Eur Commun L 54, 1-130.
• 8. European Commission Regulation No. 165/2013. Off J Eur Commun L 91, 9-12.
• 9. European Commission Regulation of 12 August 2002 No. 657/2002 implementing Council Directive 96/23/EC.
• 10. Hussein H.S., Brasel J.M.: Toxicity, metabolism and impact of mycotoxins on humans and animals. Toxicology 2001, 167, 101-134.
• 11. IARC monographs on the evaluation of carcinogenic risks to humans - some traditional herbal medicines, some mycotoxins, naphthalene and styrene 2002, 82, 169-345.
• 12. Institute for Reference Materials and Measurements. Food Safety and Quality - Geel, Belgium: Method for the simultaneous determination of deoxynivalenol, HT-2 toxin, T-2 toxin and zearalenone in unprocessed cereals and cereal-based compound animal feeds, 2011.
• 13. Kokkonem Meri K., Jestoi Marika N.: A multi - compound LC- MS/MS method for the screening of mycotoxins in grains. Food Anal Method 2009, 2, 128-140.
• 14. Kolenda M., Mroczkowski S.: Fusarium mycotoxins and methods of assessing the mycotoxicity: a review. J Centr Europ Agric 2013, 1, 169-180.
• 15. Korbas M., Ławecki T.: The possibility of reducing Fusarium blight in Poland and in the European Union. Postępy w Ochronie Roślin 2003, 41, 200-207.
• 16. Monbaliu S., Van Poucke C., Detavernier C., Dumoulin F., Van De Velde M., Schoeters E., Van Dyck S., Averkieva O., Van Peteghem C., De Saeger S.: Occurrence of mycotoxins in feed as analyzed by a multi-myeotoxin LC-MS/MS method J Agric Food Chem 2010, 58, 66-71.
• 17. Oueslati S., Romero-González R., Lasram S., Frenich A.G., Vidal J.L.: Multi-mycotoxin determination in cereals and derived products marketed in Tunisia using ultra-high performance liquid chromatography coupled to triple quadrupole mass spectrometry. Food Chem Toxicol 2012, 50, 2376-2381.
• 18. Piotrowska M.: The use of microorganisms for the removal of mycotoxins in food and feed. Post Mikrobiol 2012, 51, 109-119.
• 19. Soleimany F., Jinap S., Abas F.: Determination of mycotoxins in cereals by liquid chromatography tandem mass spectrometry. Food Chem 2012, 130, 1055-1060.
• 20. Soleimany F., Jinap S., Faridah A., Khatib A.: A UPLC - MS/MS for simultaneous determination of aflatoxins, ochratoxin A, zearalenone, DON, fumonisins. T-2 toxin and HT-2 toxin, in cereals. Food Control 2012, 25, 647-653.
• 21. Song S., Ediage E.N., Wu A., De Saeger S.: Development and application of salting-out assisted liquid/liquid extraction for multi-mycotoxin biomarkers analysis in pig urine with high performance liquid chromatography/tandem mass spectrometry. J Chromatogr A 2013, 1292, 111-120.
• 22. Sulyok M., Krska R., Schuhmacher R.: Application of a liquid chromatography tandem mass spectrometric method to multi-mycotoxin determination in raw cereals and evaluation of matrix effects. Food Additives and Contaminants 2007, 24(10), 1184-1195.
• 23. Sweeney M., Dobson A.: Mycotoxin production by Aspergillus, Fusarium and Penicillium species. Int. J. Food Microbiol 1998, 43, 141-158.
• 24. Tanaka H., Takino M., Sugita-Konishi Y., Tanaka T.: Development of a liquid chromatography/time-of-flight mass spectrometric method for the simultaneous determination of trichothecenes, zearalenone and aflatoxins in foodstuffs. Rapid Commun Mass Spectrom 2006, 20, 1422-1428.
• 25. Zöllner P., Mayer-Helm B.: Trace mycotoxin analysis in complex biological and food matrices by liquid chromatography- atmospheric pressure ionization mass spectrometry. J Chromatogr A 2006, 1136, 123-169.

Identyfikatory

DOI

10.2478/bvip-2013-0097