PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2009 | 69 | 3 |
Tytuł artykułu

Cell size and proliferation of subependymal giant cell astrocytomas are regulation by mTOR ans Ras-ERK signaling pathway

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Tuberous sclerosis complex (TSC) is characterized by cortical developmental malformations that are associated with epilepsy and appearance of benign brain tumors – subependymal giant cell astrocytomas (SEGAs) which are composed of distinct cell types, including giant cells and dysplastic neurons. TSC results from mutations in TSC1 and TSC2, which lead to the mTOR pathway activation, and p70S6 kinase and ribosomal S6 protein phosphorylation. ERK pathway is also aberrantly activated in SEGAs. Clinical trials with the mTOR inhibitor – rapamycin have demonstrated reduction in size of SEGAs, but the molecular mechanisms are unknown. In the present study, we evaluated the effects of rapamycin and the ERK pathway inhibitor – UO126 on cell size, proliferation and viability of cell cultures derived from SEGA. Rapamycin or UO126 alone did not affect viability and proliferation of SEGA cells. However, treatment with both drugs reduced proliferation of SEGA cells. Staining of F-actin revealed decrease of cell size in SEGA cultures exposed to rapamycin alone or in combination with UO126, but treatment with UO126 alone did not infl uence cell size nor morphology. Our studies demonstrate that simultaneous inhibition of both mTOR and ERK signaling pathways reduces proliferation of SEGA cells. Moreover, inhibition of mTOR signaling with rapamycin diminishes a volume of giant SEGA cells. It implicates that inhibitors of mTOR and ERK pathways should be considered for clinical trials of SEGAs.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
69
Numer
3
Opis fizyczny
p.346-347
Twórcy
autor
  • Department of Cell Biology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland
autor
  • Department of Pediatric Neurology, The Children's Memorial Health Institute, Warsaw, Poland
autor
  • Department of Pediatric Neurology, The Children's Memorial Health Institute, Warsaw, Poland
autor
  • Department of Cell Biology, Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland
Bibliografia
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-0d56badb-c645-4d3a-8f4a-ff02166906e9
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.