Induction of apoptosis in MDCK, RK13, and Neuro-2A cells infected with equine influenza virus

• Autorzy: Kwasnik M. , Rozek W. , Zmudzinski J. F.
• Języki publikacji: EN

Abstrakty

EN

The purpose of the experiment was to compare apoptosis induced by equine influenza virus (EIV A1 and EIV A2) infection in MDCK, RK13, and NEURO-2A cell lines. Flow cytometry was used to observe two symptoms of apoptosis: phosphatidylserine translocation in plasmalemnia (annexin V assay) and the fragmentation of DNA generated by endonuclease activity (TUNEL assayterminal deoxynucleotidyl transferase biotin-dUTP nick end labelling). The differences in the onset of apoptosis in the studied cells was observed. In MDCK cells infected with EIV A1 and A2, a weak signal of the phosphatidylserine translocation was observed but more cells showed the DNA fragmentation. An opposite effect was observed in case of RK 13 cells. NEURO-2A cells displayed a similar number of annexin V and TUNEL positive cells after the infection with EIV A2, while in case of EIV A1 infection, only the early symptoms of apoptosis were noted. Differences between both viral serotypes could originate from functioning of viral proteins responsible for induction or inhibition of apoptosis. The differences between cell types may result from the activation of cellular pro or anti-apoptotic mechanisms.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2013
• Tom: 57
• Numer: 1
• Opis fizyczny: p.3-7,fig.,ref.

Twórcy

autor

Kwasnik M.

• Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Rozek W.

• Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Zmudzinski J. F.

• Department of Virology, National Veterinary Research Institute, 24-100 Pulawy, Poland

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Identyfikatory

DOI

10.2478/bvip-2013-0001