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2011 | 53 | 2 |

Tytuł artykułu

Using morfological characters and simple sequence repeat (SSR) markers to characterize Tunisian fig (Ficus caricaL.) cultivars

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
We used simple sequence repeat markers and 25 morphological characters to characterize 18 Tunisian fig (Ficus carica L.) cultivars. Morphological traits suggested a high level of variation in the germplasm. Principal component analysis (PCA) differentiated the studied cultivars. In the derived dendrogram the cultivars clustered independently of their geographical origin and sex of trees. Simple sequence repeat (SSR) markers were used to compare genetic polymorphism with the observed phenotypic variation. Using six microsatellite primers, 39 alleles and 59 genotypes were identified. The high values of polymorphism information content (PIC), ranging from 0.67 to 0.85, confirmed the effectiveness of microsatellite analysis for determining molecular polymorphism and characterizing the studied cultivars. Multilocus genotyping unambiguously distinguished all the cultivars. The ability of each type of feature to differentiate cultivars of this crop is discussed.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

53

Numer

2

Opis fizyczny

p.7-14,fig.,ref.

Twórcy

autor
  • Laboratory of Molecular Genetics, Immunology and Biotechnology, Tunis Campus University, 2092 El Manar Tunis, Tunisia
autor
autor
autor
autor
autor

Bibliografia

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  • CHATTI K, SADDOUD O, SALHI-HANNACHI A, MARS M, MARRAKCHI M, and TRIFI M. 2007. Analysis of genetic diversity and relationships in a Tunisian fig (Ficus carica) germplasm collection by Random Amplified Microsatellite Polymorphisms. Journal of Integrative Plant Biology 49(3): 386–391.
  • CHATTI K, SALHI-HANNACHI A, MARS M, MARRAKCHI M, and TRIFI M. 2004a. Analyse de la diversité génétique de cultivars tunisiens de figuier (Ficus carica L.) à l'aide de caractères morphologiques. Fruits 59: 49–61.
  • CHATTI K, SALHI-HANNACHI A, MARS M, MARRAKCHI M, and TRIFI M. 2004b. Genetic diversity and phylogenetic relationships in Tunisian fig (Ficus carica L.) cultivars mediated by RAPD. Bioliogia Tunisie 1: 1–4.
  • CREGAN P.B, JARVIK I, BUSH AL, SHOEMAKER RC, LARK KG, KEHLER AL, KAYA N, VANTOAI TT, LOHNES DG, CHUNG J, and SPECHT JE. 1999. An integrated genetic linkage map soybean genome. Crop Science 39: 1464–1490.
  • DEAN RE, DAHLBERG JA, HOPKINS MS, MITCHELL CV, and KRESOVICH S. 1999. Genetic redundancy and diversity among 'orange' accessions in the US national sorghum collection assessed with simple sequence repeat (SSR) markers. Crop Science 39: 1215–1221.
  • DELLAPORTA SL, WOOD J, and HICKS JB. 1983. A plant DNA preparation, Version II. Plant Molecular Biology Reporter 4: 19–21.
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  • KHADARI B, HOCHU I, SANTONI S, and KJELLBERG F. 2001. Identification and characterization of microsatellite loci in the common fig (Ficus carica L.) and representative species of the genus Ficus. Molecular Ecology Notes 1: 191–193.
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  • KJELLBERG F, GOUYON PH, IBRAHIM M, RAYMOND M, and VALDEYRON G. 1987. The stability of the symbiosis between dioecious figs and their pollinators: a study of Ficus carica L. and Blastophaga psenes L. Evolution 41: 693–704
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  • MARS M, CHELBI T, and MARRAKCHI M. 1998 Multivariate analysis of fig (Ficus carica L.) germplasm in southern Tunisia. Acta Horticulturae 480: 75–81.
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  • PAGE RDM. 1998. TREEVIEW: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12: 357–358.
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  • RHOUMA A. 1996. Les ressources phytogénétiques oasiennes: le figuier (Ficus carica L.). Actes des 3èmes journées nationales sur les acquis de la recherche agronomique, vétérinaire et halieutique, Nabeul, Tunisie.
  • SADDOUD O, SALHI-HANNACHI A, CHATTI K, RHOUMA A, MARS M, MARRAKCHI M, and TRIFI M. 2005. Tunisian Fig (Ficus carica L.) genetic diversity and cultivars identification mediated by microsatellites markers. Fruits 60 (2): 143–153.
  • SADDOUD O, CHATTI K, SALHI-HANNACHI A, MARS M, RHOUMA A, MARRAKCHI M, and TRIFI M. 2007. Genetic diversity of Tunisian figs (Ficus carica L.) as revealed by nuclear microsatellites. Hereditas 144: 149–157.
  • SALHI-HANNACHI A, CHATTI K, SADDOUD O, MESSAOUD M, RHOUMA A, MARRAKCHI M, and TRIFI M. 2006. Genetic diversity of different Tunisian fig (Ficus carica L.) collections revealed by RAPD fingerprints. Hereditas 143: 15–22.
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Bibliografia

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