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2009 | 69 | 3 |
Tytuł artykułu

Robust in vitro migration of human umbilical cord blood neural stem cells (HUCB-NSC) toward infarcted brain tossue

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Potentially therapeutic neural stem cell line from human cord blood (HUCB-NSC) has been established in our laboratory. Reaching appropriate target by transplanted cells is a prerequisite for success of cell therapy for stroke. The question arises what is the migration potential of HUCB-NSC towards infarcted brain tissue. The migration of HUCB-NSC towards rat tissue homogenates from healthy brain (THHB) and ouabain-induced focal brain injury (THIB) obtained 6 h, 48 h and 7 days after insult was studied in vitro using transwells. Additionally the migratory activities of HUCB-NSC was checked in the presence of migration inducing proteins IGF-1 (200 ng/ml) and SDF-1 (10 ng/ml) dissolved in culture medium. Immunocytochemical analysis of migration-related receptors (CXCR-4, IGF-1R) on HUCB-NSC was performed. Results: Immunohistochemistry of HUCB-NSC unveiled expression of CXCR-4, IGF-1R. HUCB-NSC revealed robust migration toward THIB in comparison to THHB, which was most pronounced in the presence of 48 h postinfarct brain tissue (900 vs. 300 cells/well, P<0.05). The presence of IGF-1 and SDF-1 in medium increased signifi cantly HUCB-NSC migration but the effect was much weaker in comparison to injured brain tissue. The ability of robust in vitro migration of HUCB-NSC towards infarcted rat brain tissue has been confi rmed. Neither IGF-1 nor SDF-1 seems to play a pivotal role in this lesion-induced migration of HUCB-NSC. Supported by MSHE grant: N N401 332636.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
69
Numer
3
Opis fizyczny
p.294-295
Twórcy
autor
  • NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
autor
  • NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
  • NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland
Bibliografia
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-077de2b0-5424-4c2e-984f-e7405db66254
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