Porównanie różnych metod identyfikacji gatunków Phytophthora w materiale szkółkarskim

• Autorzy: Wiejacha K. , Orlikowska T.
• Języki publikacji: PL

Abstrakty

PL

Liczba wykrywanych gatunków Phytophthora w polskich szkółkach oraz zakres roślin żywicielskich zwiększają się z każdym rokiem. Straty powodowane przez fytoftorozy i brak możliwości diagnozowania na podstawie objawów chorobowych powodują konieczność zastosowania metod laboratoryjnych do szybkiej oceny materiału pod kątem zasiedlenia przez te patogeny. Ich identyfikację można przeprowadzać za pomocą analiz morfologicznych, fizjologicznych bądź biochemicznych i molekularnych. Wraz z rozwojem technik molekularnych, szczególnego znaczenia nabrało testowanie bazujące na analizie DNA [Henson, French 1993; Drenth, Irvin 2001; Taylor i in. 2001; Atkins, Clark 2004]. Wszystkie proponowane metody rutynowego testowania materiału roślinnego, gleby i wody dla celów identyfikacji lub diagnostyki powinny być szybkie, powtarzalne, wykrywające patogeny w tkankach roślinnych, glebie i wodzie, możliwie mało zależne od umiejętności wykonującego test oraz tanie.

EN

A number of Phytophthora species isolated in Polish nurseries and a range of their host plants increase in each year. The economical importance of Phyto­phthora diseases and impossibility to diagnose on the basis of symptoms, cause the necessity to use laboratory methods for quick identification and detection of these pathogens. It is possible with the aid of morphological and physiological characters or with the use of biochemical or molecular tests. Recently, the most important are the methods based on the DNA analysis [Henson, French 1993; Drenth, Irvin 2001; Taylor i in. 2001; Atkins, Clark 2004]. All the methods for mass diagnosis should be quick, repeatable, detecting a pathogen in the natural samples - plant tissue, soil and water, possibly cheap and less dependent on per­sonal skilfulness.

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2005
• Tom: 504
• Numer: 2
• Opis fizyczny: s.521-531,tab.,bibliogr.

Twórcy

autor

Wiejacha K.

autor

Orlikowska T.

Bibliografia

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