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Experimental and theoretical studies have been conducted to investigate the adsorption of atrazine in aqueous solutions by sheep manure-derived biochar synthesized at 650°C (SMB650). The results of characterization analysis showed that SMB650 possessed large specific surface area and was rich in pore structure and functional groups. The removal efficiency of atrazine by SMB650 was 95.3% under the optimum conditions, of which contact time, initial atrzaine concentration, initial solution pH, SMB650 dosage and temperature were 150 min, 1500 μg/L, 3.0, 1.6 g/L and 25ºC, respectively. The results of kinetic and isotherm studies revealed that the pseudo second-order and the Freundlich model fit the experimental data best (R2>0.98). The adsorption of atrazine onto SMB650 belonged to multi-molecular layer adsorption. The calculated thermodynamic parameters like free energy change (ΔGθ), enthalpy change (ΔHθ) and entropy change (ΔSθ) were -7.8730 to -6.2976 kJ/mol, 17.2179 kJ/mol and 0.0788 kJ/(mol·K), respectively, indicating that the adsorption process of atrazine onto SMB650 was spontaneous, endothermic and entropy-increased. The present study showed that the sheep manure-derived biochar could be used as a promising adsorbent for the removal of atrazine from aqueous solutions.
To fully understand the sensitivity of photosystem II (PSII) activity of immature and mature leaves in desert plants to light stress, we explored the chlorophyll a fluorescence kinetics in Alhagi sparsifolia Shap. Plants. Results show that the chlorophyll fluorescence transient intensity of both immature and mature leaves was significantly higher under shaded conditions (about 50 % of natural light intensity) than that under ambient light conditions (100 % of natural light intensity). The energy fluxes per reaction center and cross section of immature and mature leaves were higher under shaded conditions than of those under ambient conditions. By contrast, the quantum yields and efficiencies and performance indices under shaded conditions were lower than of those under ambient conditions. However, the chlorophyll fluorescence transient intensity and parameters of both immature and mature leaves significantly changed under natural light exposure. These results suggest that the PSII activity of immature and mature leaves under shaded irradiance was enhanced, but the PSI acceptor side was affected as compared with that under ambient conditions. Activity of the donor side of PSII of immature leaves was higher than that of mature leaves in both shaded and ambient light conditions, and the electron transport activity at the acceptor side of PSII of both immature and mature leaves was inhibited by high irradiance after being kept in the shade. Furthermore, immature leaves suffered from severe inhibition than did mature leaves after exposure to ambient conditions. This study showed that immature leaves were more sensitive than mature leaves were to light stress.
 Hypericum perforatum is well known for its antidepressant and anti-inflammatory activities, for which hypericin and its derivatives are indicated to be the most active compounds. Hypericin synthase (Hyp-1) is the only protein proven to catalyze the synthesis of hypericin. In this study, the full-length cDNA of Hyp-1 was chemically synthesized according to the Hyp-1 sequence in GenBank (accession no. AY148090) and then cloned into the plasmid pET22b. Hyp-1 was expressed in Escherichia coli BL21 (DE3) and purified with a Ni-NTA column. The purified protein was used to immunize New Zealand white rabbits, from which an antiserum was purified by protein G affinity chromatography. The polyclonal antibody against Hyp-1 provides a valuable tool for the study of hypericin biosynthesis in H. perforatum. Expression of Hyp-1 and the cellular distribution of hypericin were analyzed in different organs of red-pigmented H. perforatum plantlets. The black glands were not the only site of hypericin accumulation and the results indicated that hypericin might be synthesized in mesophyll cells or in tissues of the root and/or stem and then transported to the glands. This work provides a foundation for further investigation of the regulatory mechanism of hypericin synthesis during the development of H. perforatum.
Lead (Pb) contamination of urban soil is a threat to human health, and reducing the risk of Pb-contaminated soil is a continuing international concern. Soil stabilization technique has been considered as apromising remediation technique to facilitate the immobilization of Pb in soil. The purpose of this study was to evaluate the effects of different amendments, including H₃PO₄, Ca(H₂PO₄)₂, H₃PO₄+Ca(H₂PO₄)₂, and zeolite on Pb levels in two naturally contaminated urban soils. Pb speciation and bioavailability was evaluated by the sequential extraction test (SET) and the toxicity characteristic leaching procedure (TCLP) after a two-month incubation. The application of phosphorus amendments significantly reduced the TCLP-Pb concentration in two soil types and the SET-Pb in Soil 1 (alkaline but with high Pb level), whereas the zeolite amendment diminished the SET-Pb in two soil types and the TCLP-Pb in Soil 1. Nevertheless, regardless of the soil type, the application of phosphorus amendments resulted in a significant increase of residual Pb. These phosphate amendments may be a viable strategy in the in situ remediation of Pb contamination in urban soils.
Nitrogen (N) is one of the most important limiting factors for plant growth and development. Amino acids are the major source of organic N, which is converted from inorganic N absorbed by plant roots from the soil. Amino acid transporters are the principal mediators of organic N distribution and important regulators of resource allocation in plants. Although the complete genomic sequence of rice has already been released, there is still little known about amino acid transporter genes in rice. In this study, 79 OsAAT genes were identified by a database search of the rice genome based upon HMM profiles. A bioinformatics analysis of the complete set of OsAAT genes is presented, including chromosomal location, phylogenetic analysis, gene structure, protein analysis, conserved motifs, protein structures and cis-element analysis of the promoters. In addition, the comprehensive expression profile of OsAAT genes in rice tissues/organs under N starvation conditions was investigated by real-time PCR analysis. Diverse expression patterns of OsAAT genes indicated diverse biological functions of the amino acid transporters and the important roles of OsAAT genes in N uptake, metabolism and distribution during N starvation. The evaluation of yield and carbon (C) and N content of osaat knockout mutants also suggested the important roles of the OsAAT5, OsAAT7, OsAAT24, OsAAT49 and OsAAT60 genes in yield and biomass production and C and N metabolism and distribution in rice plants.
Sheep manure was used to prepare biochar under pyrolysis temperature of 600ºC. The structural features of biochar were characterized by elemental analysis, BET analysis and scanning electron microscopy. The effects of pH, biochar dosage, adsorption time, temperature on adsorption of methyl orange (MO) in water by sheep manure biochar, as well as its adsorption mechanism, were investigated via batch experiments. The results showed that the sheep manure biochar had large specific surface area, abundant hole structure and high aromaticity and polarity. When temperature was 25ºC, MO concentration was 20 mg/L, initial pH was 4.0, and biochar dosage was 0.6 g/L, the adsorption achieved balance at about 250min, and the MO removal rate reached to 92.55%. Pseudo second-order kinetic model and Langmuir model could more accurately describe the adsorption behavior of MO onto sheep manure biochar, and the theoretical maximum adsorption capacity was 42.513 to 45.563 mg/g. Besides, the process is a favorable adsorption. Thermodynamic studies showed that the adsorption was a spontaneous, endothermic and entropy-increasing process. Sheep manure biochar could be used as a good adsorption material for MO in water, which achieved the goal of controlling waste by waste.
Anaerobic ammonium oxidation (anammox) is an efficient and promising pathway for nitrogen removal from wastewater, but its application is usually confined by the low growth rate and susceptibility to surroundings of anammox bacteria. In this study, cultured anammox sludge was immobilized using polyvinyl alcohol (PVA)-sodium alginate (SA) gel and put into a lab-scale column reactor at a packing ratio of 20%, and nitrogen removal performance was evaluated at two hydraulic retention times (HRTs). Anammox was rapidly initiated in the reactor, with ammonium and nitrite removal efficiency reaching 82.3% and 84.7% after an operation period of 10 d. Nitrogen removal efficiency declined greatly after the reduction of HRT from 24 h to 12 h, but then recovered quickly, with an average TN removal rate of 84.5% and 0.43 kg·m⁻³·d⁻¹ achieved under the steady operational state. The immobilized anammox reactor performed significantly better and was more stable in nitrogen removal than that with anammox sludge inoculated directly, indicating the superiority of cell entrapment of anammox biomass in addition to its easy reservation. Nitrogen removal in the reactor increased after stable operation with the HRT. It has potential to apply immobilized anammox sludge entrapped by PVA-SA gel for the convenient establishment of an anammox reactor with stable and high nitrogen removal rates.
Two trials were conducted to investigate the effect of a combination of essential oils (CEO) along with fumarate on in vitro rumen fermentation. In trial 1, the essential oil (EO) from thyme, oregano, cinnamon and lemon were mixed at five different ratios. The CEO were applied at levels of 0-500 mg/l. Addition of CEO decreased gas, methane, total volatile fatty acid (VFA) production at 24 h incubation in a dose-dependent manner. Methane tended to decrease much more than total VFA and gas at the same EO level. The mixture of oils at an equal ratio at 500 mg/l that decreased methane much more than VFA was chosen as the optimal combination. In trial 2, the optimal combination was used with 0, 5, 10 or 15 mmol/l of monosodium fumarate. Addition of fumarate further decreased methane production, with 10 mmol/l fumarate resulting in the largest reduction (80.2%) and the smallest decrease in total VFA (5.7%) and gas production (16.7%). Quantification of several ruminal microbe populations by RT-PCR showed that the optimal combination sharply decreased ruminal protozoa; the populations of fungi and fibrolytic bacteria were also decreased. In summary, at an appropriate level, CEO can inhibit methane production. Inclusion of fumarate can further decrease it, which is attributed mainly to inhibition of protozoa and methanogens.
The degradation of atrazine (ATZ) by ultraviolet/peroxymonosulfate (UV/PMS) under different conditions was investigated in phosphate buffer, and the degradation mechanism and kinetics were discussed. The results showed that the degradation rate of 2.5 μmol/L ATZ in UV/PMS system was 97.63% in 20 min when the reaction temperature was 20ºC, the concentration of PMS was 20 μmol/L and the UV intensity was 50 mW/cm2 in pH7 phosphate buffer. The mechanism analysis showed that PB with partial alkalinity promoted the degradation of ATZ by UV/PMS more than that with acidic PB. The effect of PB with alkaline conditions on the degradation of ATZ by UV/PMS was more complicated and mainly related to the state of phosphate ions. The UV/PMS system contained both HO• and SO4-•, and the ratio of HO•, SO4-•and UV-degraded ATZ was nearly 1:1 in pH7 PB. Inorganic anions experiments showed that Cl- and HCO3- inhibited the degradation of ATZ under UV/PMS, and the inhibitory effect of Cl- was more obvious. NO3- promoted the degradation of ATZ by UV/PMS. Kinetic analysis showed that UV/PMS degradation of ATZ reaction kinetics was more in line with the quasi first-order reaction kinetics, the inhibition effect of the same concentration of ETA and Clon UV/PMS degradation of ATZ are the same, and UV/PMS degradation of ATZ decreased by 38.54% and 36.29% respectively. The addition of NO3- increased the rate of degradation of ATZ by UV/PMS by 31.21%. By LC-MS analysis, 5 kinds of production m/z and 6 kinds of products were obtained.
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