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The effect of 20 new hydrazine derivatives on Musca domestica was determined by topical application method. As standard hydrazides of the cyanoacetic and isonicotinic acids as well as hexochlorocyclohexane (BHC with isomer γ contents 99-100%, m.p. 112-113°) and dichlorodiphenyltrichloroethane (DDT, m.p. 107-109°) produced by Chemical Plants "Azot" Jaworzno were used. It was stated that among 20 new compounds only 5 acted as insecticides on adult M. domestica individuals viz. N-cyanoacetyl-N'-hippurylhydrazine, phenylhydrazide of methyl ester of succinic acid, phynelhydrazide-N-propylamide of succinic acid, o-nitrophenylhydrazide of isonicotinic acid, p-bromophenylhydrazide hydrochloride of isonicotinic acid (Table 3). Their action on the third - rate larvas of M. domestica was evaluated by modified Gliwic method; determinations were repeated twice with three different compound concentrations, the mortal doses 50% were calculated according to Kadłubowski. It was found that larva-killing effect showed the o-nitrophenylhydrazide of isonicotinic acid only; lindane acted on larvas a little weaker. None of investigated new or standard compounds showed egg-killing activity (Table 3). Special consideration deservs the o-nitrophenylhydrazide of isonicotinic acid in spite of its weaker activity on adult M. domestica individuals than DDT, it is remarkably less toxic for vertebrates (white mouse LD₅₀ = 403 ± 25 mg/kg), its larva-killing action is more stronger than that of lindane.
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The antihelminthic effect was determined in Enchytraeus sp. No 80 strain (20 helminths in 2 ml of 0.5% NaCl solution and 0.01 phosphate buffer at 7.4, room temperature, evaluation after 24 hours) and Ascaris lumbricoides suis (5 females in 200 ml of Ringer's solution in the case of thermolability, at 37°C, after 24 hours) and by kymography on the izolated preparation of Ascaris lumbricoides suis (Ringer's solution, 37°C, reaction within 10 minutes, calculated in relation to standard reaction taken as 100). DL₅₀ was calculated by own method (Wiad. Parazytol., 1961). It has been found that the strongest action on Enchytraeus is exerted by glycol methylene ether (DL₅₀ = 0.19±0.21 m) and piperazine adipinate (0.15 ± 0.63 m); twice weaker is the action of glycol ethylene ether, eight times weaker that of thylene and propylene glycols. In Ascaris lumbricoides the strongest antihelminthic action was exhibited by ethylene glycol ethyl ether (0.47 ± 0.01 m), twice weaker by methylene ether, 3-4 times weaker by ethylene and propylene glycols. Propylene glycol and methylene ether depressed the tonus of A lumbricoides preparation by 50% within 10 minutes at concentrations approaching lethal ones, whereas the remaining glycols acted twice weaker. Piperazine adipinate had neither killing action on the parasite nor depressing effect on the tonus of the preparation. The relative toxicity index (DL₅₀ for mouse: DL₅₀ for helminth) is the most favourable for propylene glycol (130), then for ethylene glycol (65), ethylene glycol ethyl (52) and methyl ether (30).
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