Effects of ascorbic acid and alpha-tocopherol on collagen fibril stereological parameters in rabbits

• Autorzy: Sarrafzadeh-Rezaei F. , Farshid A.-A. , Dalir-Naghadeh B. , Saifzadeh S. , Abbasnia P. , Jarolmasjed S.-H.
• Języki publikacji: EN

Abstrakty

EN

Twenty adult (8 to 10 months old) male New Zealand White rabbits were divided randomly into four equal groups. After preparation of each rabbit for surgical procedure, the right deep digital flexor tendon was crushed in a standard method. Then the limb was fixed with external coaptation for 7 d. The post-operative treatment in each group included: no treatment (CN), ascorbic acid, 100 mg.kg⁻¹, i.p. (AA), α -tocopherol, 20 mg.kg⁻¹, i.m. (AT), and both vitamins simultaneously (CM) for nine consecutive days. At the 13th d after surgery each rabbit was euthanized and tissues from the crushed tendon were prepared for ultrastructural evaluation by transmission electron microscopy. The median values of collagen fibril diameter in the AT group were the highest; group AA was more than the CN and CM groups; and group CM was the least. The results obtained indicated a significant positive effect of ascorbic acid and α-tocopherol on collagen fibril structural properties and tendon healing. However, combination of both vitamins had no synergistic effect on tendon healing and even significantly decreased the effect in comparison to each individual vitamin (P<0.05).

Słowa kluczowe

EN

alpha-tocopherol; antioxidative function; ascorbic acid; collagen; collagen fibril; healing; rabbit; tendon

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 3
• Opis fizyczny: p.377-382,fig.,ref.

Twórcy

autor

Sarrafzadeh-Rezaei F.

• Department of Clinical Sciences, Urmia University, 57153-1177 Urmia, Iran

autor

Farshid A.-A.

autor

Dalir-Naghadeh B.

autor

Saifzadeh S.

autor

Abbasnia P.

autor

Jarolmasjed S.-H.

Bibliografia

• 1. Binkley J M., Peat M.: The effects of immobilization on the ultrastructure and mechanical properties of the medial collateral ligament of rats. Clin Orthop Relat Res 1986, 203, 301-308.
• 2. Blankevoort L., Huiskes R.: A mechanism for rotation restraints in the knee joint. J Orthop Res 1996, 14, 676- 679.
• 3. Card J.W., Leeder R.G., Racz W.J., Berin J.F., Bray T.M., Massey T.E.: Effect of dietary vitamin E supplementation on pulmonary morphology and collagen deposition in amiodarone- and vehicle-treated hamsters. Toxicology 1999, 133, 75-84.
• 4. Chhabra A., Tsou D., Clark R.T., Gaschen V., Hunziker E.B., Mikic B.: GDF-5 deficiency in mice delays Achilles tendon healing. J Orthop Res 2003, 21, 826-835.
• 5. Comerford E.J., Tarlton J.F., Wales A., Bailey A.J., Innes J.F.: Ultrastructural differences in cranial cruciate ligaments from dogs of two breeds with a differing predisposition to ligament degeneration and rupture. J Comp Pathol 2006, 134, 8-16.
• 6. Constantinescu M.A., Greenwald D.P., Amarante M.T.J., Nishioka N.S., May, J.W. Jr.: Effects of laser versus scalpel tenolysis in the rabbit flexor tendon. Plast Reconstr Surg 1996, 97, 595-601.
• 7. Durak K., Sonmez G., Sarisozen B., Ozkan S., Kaya M., Ozturk C.: Histological assessment of the effect of α-tocopherol on fracture healing in rabbits. J Int Med Res 2003, 31, 26-30.
• 8. Elliott W.L.: Role of vitamin C in collagen biosynthesis and connective tissue health. J Bodywork Movement Ther 2002, 6, 221-224.
• 9. Galeano M., Torre V., Deodato B., Campo G.M., Colonna M., Sturiale A., Squadrito F., Cavallari V., Cucinotta D., Buemi M., Altavilla D.: Raxofelast, a hydrophilic vitamin E-like antioxidant, stimulates wound healing in genetically diabetic mice. Surgery 2001, 129, 467-477.
• 10. Greenwald D.P., Sharzer L.A., Padawer J., Levenson S.M., Seifter E.: Zone II flexor tendon repair: effects of vitamins A, E, ß-carotene. J Surg Res 1990, 94, 98- 102.
• 11. Lavagnino M., Arnoczky S.P., Frank K., Tian T.: Collagen fibril diameter distribution does not reflect changes in the mechanical properties of in vitro stress- deprived tendons. J Biomech 2005, 38, 69-75.
• 12. Luo J., Mass D.P., Phillips C.S., He T.C.: The future of flexor tendon surgery. Hand Clin 2005, 21, 267-273.
• 13. MacKay D., Miller A.L.: Nutritional support for wound healing. Altern Med Rev 2003, 8, 359-377.
• 14. Mazzotta M.Y.: Nutrition and wound healing. J Am Pediatr Med Assoc 1994, 84, 456-462.
• 15. Mikic B., Schalet B.J., Clark R.T., Gaschen V., Hunziker E.B.: GDF-5 deficiency in mice alters the ultrastructure, mechanical properties and composition of the Achilles tendon. J Orthop Res 2001, 19, 365- 371.
• 16. Mori Y., Hatamochi A., Takeda K., Ueki H.: Effect of tretinoin tocoferil on gene expression of the extracellular matrix components in human dermal fibroblasts in vitro. J Dermatol Sci 1994, 8, 233-238.
• 17. Parry D.A.D., Barnes G.R.G., Craig A.S.: A comparison of the size distribution of collagen fibrils in connective tissues as a function of age and a possible relation between fibril size distribution and mechanical properties. Proc R Soc Lond B Biol Sci 1978, 203, 305-321.
• 18. Parry D.A.D., Craig A.S., Barnes G.R.G.: Tendon and ligament from the horse: an ultrastructural study of collagen fibrils and elastic fibers as a function of age. Proc R Soc Lond B Biol Sci 1978, 203, 293-303.
• 19. Patterson-Kane J.C., Wilson A.M., Firth E.C., Parry D.A.D., Goodship A.E.: Exercise-related alterations in crimp morphology in the central regions of superficial digital flexor tendons from young thoroughbreds: a controlled study. Equine Vet J 1998, 30, 61-64.
• 20. Ramirez A., Schwane J.A., McFarland C., Starcher B.: The effect of ultrasound on collagen synthesis and fibroblast proliferation in vitro. Med Sci Sports Exerc 1997, 29, 326-332.
• 21. Robinson P.S., Lin T.W., Jawad A.F., Iozzo R.V., Soslowsky L.J.: Investigating tendon fascicle structure- function relationships in a transgenic-age mouse model using multiple regression models. Ann Biomed Eng 2004, 32, 924-931.
• 22. Sarisozen B., Durak K., Dincer G., Bilgen O. F.: The effects of vitamins E and C on fracture healing in rats. J Int Med Res 2002, 30, 309-313.
• 23. Sarrafzadeh-Rezaei F.: The effects of electrical stimulation and phenytoin therapy on the experimentally induced tendon injuries in rabbit. Thesis, University of Shiraz, Iran, 1993.
• 24. Sarrafzadeh-Rezaei F., Hobbenaghi R., Behfar M.: The effect of Flunixin meglumine on tendon healing in rabbit: quantitative histopathological study. J Vet Pharmacol Ther 2006, 29 (Suppl 1), 93-94.
• 25. Sharma P., Maffull N.: Tendon injury and tendinopathy: healing and repair. J Bone Joint Surg 2005, 87-A, 187-202.
• 26. Södersten F., Ekman S., Eloranta M.L., Heinegard D., Dudhia J., Hultenby K.: Ultrastructural immunolocalization of cartilage oligomeric matrix protein (COMP) in relation to collagen fibrils in the equine tendon. Matrix Biol 2005, 24, 376-385.
• 27. Terova G., Saroglia M., Papp Z.Gy., Cecchini S.: Dynamics of collagen indicating amino acids in embryos and larvae of sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata), originated from broodstocks fed with different vitamin C content in the diet. Comp Biochem Physiol A Mol Integr Physiol 1998, 121, 111-118.
• 28. Williams I.F., Craig A.S., Parry D.A.D., Goodship A.E., Shah J., Silver I.A.: Development of collagen fibril organization and collagen crimp patterns during tendon healing. Int J Biol Macromol 1985, 7, 275-282.
• 29. Yamamoto E., Hata D., Kobayashi A., Ueda H., Tangkawattana P., Oikawa M., Takehana K.: Effect of ß-aminopropionitrile and hyaluronic acid on repair of collagenase-induced injury of rabbit Achilles tendon. J Comp Pathol 2002, 126, 161-170.
• 30. Yasuda K., Erickson A.R., Beynnon B.D., Johnson R.J., Pope M.H.: Dynamic elongation behavior in the medial collateral and anterior cruciate ligaments during lateral impact loading. J Orthop Res 1993, 11, 190-198.