Metody skriningu bakteriocynogennych bakterii fermentacji mlekowej

• Autorzy: Sip A. , Krasowska M. , Wieckowicz M. , Grajek W.

Warianty tytułu

EN

Methods to screen bacteriocinogenic lactic acid bacteria

• Języki publikacji: PL

Abstrakty

PL

W pracy omówiono metody poszukiwania bakterii fermentacji mlekowej zdolnych do syntezy bakteriocyn. Przedstawiono trzy tradycyjne procedury skriningowe. Opisano także sposoby identyfikacji LAB oraz detekcji wytwarzanych przez nie bakteriocyn. Zwrócono uwagę na złożony (wielopłaszczyznowy) charakter tradycyjnych procedur skriningowych i wyjaśniono przyczyny niskiej ich efektywności. Omówiono ponadto rolę analizy metagenomowej oraz fluorescencyjnej hybrydyzacji in situ w pracach skriningowych. Podkreślono, że techniki te mogą być stosowane we wstępnych etapach skriningu do typowania środowisk bogatych w bakteriocynogenne LAB, zwiększając tym samym prawdopodobieństwo pozyskania ze środowiska naturalnego drobnoustrojów zdolnych do syntezy aktywnych bakteriocyn. W końcowej części pracy dokonano porównania tradycyjnych i nowoczesnych procedur skriningowych.

EN

In this paper, some screening methods to detect LAB able to synthesize bacteriocins were discussed. Three conventional screening procedures were presented. Furthermore, the methods to identify LAB and to detect bacteriocins produce by LAB were described. The complex (multipronged) character of conventional screening procedures was emphasized and the reasons for their low effectiveness were explained. In addition, the role of both the metagenomic analysis and the fluorescent hybridization in situ in screening procedures was detailed. It was emphasized that those techniques might be applied at initial screening stages to determine habitats rich in bacteriocinogenic LAB, thereby increasing the probability of isolating the bacteriocin-producing microorganisms from natural systems. In the final part of the paper, the conventional and novel screening procedures were compared.

Słowa kluczowe

PL

bakterie kwasu mlekowego; bakteriocyny; fluorescencyjna hybrydyzacja in situ; skrining; zywnosc; przedluzanie trwalosci; aktywnosc biologiczna

• Wydawca: -
• Czasopismo: Żywność Nauka Technologia Jakość
• Rocznik: 2009
• Tom: 16
• Numer: 1
• Opis fizyczny: s.5-26,tab.,bibliogr.

Twórcy

autor

Sip A.

• Katedra Biotechnologii i Mikrobiologii Żywności, Wydz. Nauk o Żywności i Żywieniu, Uniwersytet Przyrodniczy w Poznaniu, ul. Wojska Polskiego 48, 60-627 Poznań

autor

Krasowska M.

• Katedra Biotechnologii i Mikrobiologii Żywności, Wydz. Nauk o Żywności i Żywieniu, Uniwersytet Przyrodniczy w Poznaniu, ul. Wojska Polskiego 48, 60-627 Poznań

autor

Wieckowicz M.

• Katedra Biotechnologii i Mikrobiologii Żywności, Wydz. Nauk o Żywności i Żywieniu, Uniwersytet Przyrodniczy w Poznaniu, ul. Wojska Polskiego 48, 60-627 Poznań

autor

Grajek W.

• Katedra Biotechnologii i Mikrobiologii Żywności, Wydz. Nauk o Żywności i Żywieniu, Uniwersytet Przyrodniczy w Poznaniu, ul. Wojska Polskiego 48, 60-627 Poznań

Bibliografia

• [1] Abee T., Klaenhammer T.R., Letellier L.: Kinetic studies of the action of lactacin F, a bacteriocin produced by Lactobacillus johnsonii that forms poration complexes in the cytoplasmic membrane. Appl. Environ. Micobiol., 1994, 60, 1006-1013.
• [2] Amann R., Fuchs, B.M., Behrens S.: The identification of microorganisms by fluorescence in situ hybridization. Curr. Opin. Biotechnol., 2001, 12, 231-236.
• [3] Ben Belgacem Z., Ferchichi M., Prévost H., Dousset X., Manai M.: Screening for anti-listerial bacteriocin-producing lactic acid bacteria from “Gueddid” a traditionally Tunisian fermented meat. Meat Sci., 2008, 78, 513-521.
• [4] Bouksaim M., Fliss I., Meghrous J., Simard R. E., Lacroix C.: Immunodot detection of nisin Z in milk and whey using enhanced chemiluminescence. J. Appl. Microbiol., 1998, 84, 176-184.
• [5] Bouksaim M., Lacroix C., Audet P., Simard R.E.: Effects of mixed starter composition on nizin Z production by Lactococcus lactis subsp. lactis biovar. diacetylactis UL 719 during production and ripening of Gouda cheese. Int. J. Food Microbiol., 2000, 59, 141-156.
• [6] Budde B.B., Rasch M.: A comparative study on the use of flow cytometry and colony forming units for assesment of the antibacterial effect of bacteriocins. Int. J. Food Microbiol., 2001, 63, 65-72.
• [7] Cabo M.L., Murado M.A., González M.P., Pastoriza L.: A method for bacteriocin quantification. J. Appl. Microbiol., 1999, 87, 907-914.
• [8] Chen H., Hoover D.G.: Bacteriocins and their food applications. Comp. Rev. Food Sci. Food Safety, 2003, 2, 82-100.
• [9] Cleveland J., Montville T.J., Nes I.F., Chkindas M.L.: Bacteriocins: safe, natural antimicrobials for food preservation. Int. J. Food Microbiol., 2001, 71, 1-20.
• [10] Daoudi L., Turcotte C., Lacroix C., Fliss I.: Production and characterization of anti-nisin Z monoclonal antibodies: suitability for distinguishing active from inactive forms trough a competitive enzyme immunoassay. Appl. Microbiol. Biotechnol., 2001, 56, 114-119.
• [11] De Boer E., Beumer R.R.: Methodology for detection and typing of foodborne microorganisms. Int. J. Food Microbiol., 1999, 50, 119-130.
• [12] De la Fuente-Salcido N., Salcedo-Hernández R., Alanís-Guzmán Ma. G., Bideshi D.K., Barboza- Corona J.E.: A new rapid fluorogenic method for measuring bacteriocin activity. J. Microbiol. Methods, 2007, 70, 196-199.
• [13] De Martinis E.C.P., Publio M.R.P., Santarosa P.R., Freitas F.Z.: Antilisterial activity of lactic acid bacteria isolated from vacuum-packaged Brazilian meat and meat products. Brazilian J. Microbiol., 2001, 32, 32-37.
• [14] De Vos W.M., Mulders J.W.M., Siezen R.J., Hugenholtz J., Kuipers O.P.: Properties of nisin Z and distribution of its gene, nisZ, in Lactococcus lactis. Appl. Environ. Microbiol., 1993, 59, 213-218.
• [15] Deja-Sikora E., Sikora M., Golebiewski M., Tretyn A.: Metagenomic libraries as sources of genes useful for biotechnology, Biotechnol., 2007, 4, 125-139.
• [16] Delgado A., Brito D., Fevereiro P., Tenreiro R., Peres C.: Bioactivity quantification of crude bacteriocin solutions. J. Microbiol. Methods, 2005, 62, 121-124.
• [17] Dewan S., Tamang J.P.: Dominant lactic acid bacteria and their technological properties isolated from the Himalayan ethnic fermented milk products. Antonie van Leeuwenhoek, 2007, 92, 343-352.
• [18] Eijsink V. G. H., Skeie M., Middelhoven P. H., Brurberg M. B., Nes I. F.: Comparative studies of class IIa bacteriocins of lactic acid bacteria. Appl. Environ. Microbiol., 1998, 64, 3275-3281.
• [19] Font de Valdez G., Lorca G., Taranto M.P.: Conductimetric method for evaluating inhibition of Listeria monocytogenes. In: Food Microbiology Protocols - ed. by Spencer J. F. T. i Ragout de Spencer A.L., Humana Press, 2001, 55-59.
• [20] Franz C.M.A., Du Toit M., von Holy A., Schillinger U., Holzapfel W.H.: Production of nisin-like bacteriocins by Lactococcus lactis strains isolated from vegetables. J. Basic Microbiol., 1997, 37, 187-196.
• [21] Galvez A., Abriouel H., Lopez R.L., Omar N.B.: Bacteriocin-based strategies for food biopreservation. Int. J. Food Microbiol., 2007, 12, 51-70.
• [22] Ghrairi T., Manai M., Berjeaud J.M., Frère J.: Antilisterial activity of lactic acid bacteria isolated from rigouta, a traditional Tunisian cheese. J. Appl. Microbiol., 2004, 97, 621-628.
• [23] Giraffa G., Neviani E.: DNA-based, culture-independent strategies for evaluating microbial communities in food-associated ecosystems. Int. J. Food Microbiol., 2000, 67, 19-34.
• [24] Hastings J., Baldwin T., Nicoli M.: Bacterial luciferase: assay, purification and properties. Methods Enzymol., 1978, 57, 135-152.
• [25] Héchard Y., Sahl H.G.: Mode of action of modified and unmodified bacteriocins from gram-positive bacteria. Biochimie, 2002, 84, 545-557.
• [26] Holck A., Axelsson L., Schillinger U.: Divergicin 750, a novel bacteriocin produced by Carnobacterium divergens 750. FEMS Microbiol. Lett., 1996, 136, 163-168.
• [27] Hoover D.G., Dishert K.J., Hermes M.A.: Antagonistic effect of Pediococcus spp. against Listeria monocytogenes. Food Biotechnol., 1989, 3, 183 - 196.
• [28] Hoover D.G., Harlander S.K.: Screening methods for detecting bacteriocin activity. In: Bacteriocins of lactic acid bacteria - ed. by Hoover D.G., Steenson L.R., Food Science and Technology, Academic Press, 1993, pp. 23-39.
• [29] Jack R.W., Tagg J.R., Ray B.: Bacteriocins of gram-positive bacteria. Microbiol. Rev., 1995, 59, 171-200.
• [30] Klaenhammer T.R.: Genetics of bacteriocins produced by lactic acid bacteria. FEMS Microbiol. Rev., 1993, 12, 39-86.
• [31] Kraszewska J., Wzorek W., Sztando E., Raczyńska-Cabaj A.: Aktywność antagonistyczna bakterii fermentacji mlekowej z gatunku Lactobacillus plantarum. Acta Sci. Pol., Technol. Aliment., 2005, 4, 39-52.
• [32] McElroy W., DeLuca M.: Firefly luminescence. In: Chemi- and bioluminescence - ed. by Burr J., Marcell Dekker, New York, 1985, pp. 387-399.
• [33] Messi P., Bondi M., Sabia C., Battini R., Manicardi G.: Detection and preliminary characterization of a bacteriocin (plantaricin 35d) produced by a Lactobacillus plantarum strain. Int. J. Food Microbiol., 2001, 64, 193-198.
• [34] Morgan S., Ross R.P., Hill C.: Bacteriolytic activity caused by the presence of a novel lactococcal plasmid encoding lactococcins A, B and M. Appl. Environ. Microbiol., 1995, 61, 2995-3001.
• [35] Moschetti G., Blaiotta G., Villani F., Copolla S.: Nisin-producing organisms during traditional “Fior di latte” cheese-making monitored by multiplex-PCR and PFGE analyses. Int. J. Food Microbiol., 2001, 63, 109-116.
• [36] Moter A., Goebel U.B.: Fluorescence in situ hybridization (FISH) for direct visualization of microorganisms. J. Microbiol. Methods, 2000, 41, 85-112.
• [37] Mugochi T., Nandakumar M.P., Zvauya R., Mattiasson B.: Bioassay for the rapid detection of bacteriocins in fermentation broth. Biotech. Lett., 2001,23, 1243-1247.
• [38] Muriana P.M., Luchansky J.B.: Biochemical methods for purification of bacteriocins. In: Bacteriocins of lactic acid bacteria - ed. by Hoover D.G., Steenson L.R., Food Science and Technology, Academic Press, 1993, pp. 41-61.
• [39] Ohmomo S., Nitisinprasart S., Hiranpradit S.: How to screen favorable lactic acid bacteria strain, from the case of bacteriocin producing strain and silage fermentation starter strain. The world of indigenous fermented foods for technology development and food safety. I-6. Kasetsart University, Bangkok, August, 2003. Proceeding CD-ROM
• [40] O’Sullivan R., Ross R.P., Hill C.: Potential of bacteriocin-producing lactic acid bacteria for improvements in food safety and quality. Biochimie, 2002, 84, 593-604.
• [41] Papagianni M., Avramidis N., Filioussis G., Dasiou D., Ambrosiadis I.: Determination of bacteriocin activity with bioassays carried out on solid and liquid substrates: assessing the factor “indicator microorganism”. Microb. Cell Factories, 2006, 5, 30-44.
• [42] Parente E., Hill C.: Characterisation of enterocin 1146, a bacteriocin from Enterococcus faecium inhibitory to Listeria monocytogenes. J. Food Prot., 1992, 55, 497-502.
• [43] Poeta P., Costa D., Rojo-Bezares B., Zarazaga M., Klibi N., Rodrigues J., Torres C.: Detection of antimicrobial activities and bacteriocin structural genes in faecal enterococci of wild animals. Microbiol Res., 2007, 162, 257-263.
• [44] Robertson A., Tirado C., Lobstein T., Jeremini M., Knai C., Jensen H.J., Ferro-Luzzi A., James W.P.T.: Food and health in Europe: a new basis for action. WHO Regional Publications, European Series, 2004, vol. 96, Geneva.
• [45] Rodriguez J.M., Cintas L.M., Casaus P., Suarez A., Hernandez P.E.: PCR detection of the lactocin S structural gene in bacteriocin-producing Lactobacilli from meat. Appl. Environ. Microbiol,1995, 61, 2802-2805.
• [46] Rose N.L., Sporns P., McMullen L.M.: Detection of bacteriocins by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Appl. Environ. Microbiol., 1999, 65, 2238-2242.
• [47] Sabia C., Messi P., de Niederhäusern S., Manicardi G., Bondi M.: Study of two bacteriocins produced by Enterococcus casseliflavus and Ent. faecalis. Lett. Appl. Microbiol., 2004, 38, 99-105.
• [48] Simon L., Fremaux C., Cenatiempo Y., Berjeaud J.-M.: Luminescent method for the detection of antibacterial activities. Appl. Microbiol. Biotechnol., 2001, 57, 757-763.
• [49] Streit W.R., Schmitz R.A.: Metagenomics - the key to the uncultured microbes. Curr. Opin. Microbiol., 2004, 7, 492-498.
• [50] Strus M.: Nowa metoda oceny antagonistycznego działania bakterii kwasu mlekowego (LAB) na wybrane, chorobotwórcze bakterie wskaźnikowe. Med. Dośw. Microbiol., 1998, 50, 123-130.
• [51] Temmerman R., Huys G., Swings J.: Identification of lactic acid bacteria: culture-dependent and culture-independent methods. Trends Food Sci. Technol., 2004, 15, 348-359.
• [52] Todorov S.D., Dicks L.M.T.: Screening for bacteriocin-producing lactic acid bacteria from boza, a traditional cereal beverage from Bulgaria. Comparison of the bacteriocins. Process Biochem., 2006, 41, 11-19.
• [53] Vesterlund S., Paltta J., Lauková A., Karp M., Ouwehand A.C.:. Rapid screening method for the detection of antimicrobial substances. J. Microbiol. Methods, 2004, 57, 23-31.
• [54] Wagner M., Horn M., Daims H.: Fluorescence in situ hybridization for the identification and characterization of prokaryotes. Curr. Opin. Microbiol., 2003, 6, 302-309.
• [55] Wahlström G., Saris P.E.J.: A nisin bioassay based on bioluminescence. Appl. Environ. Microbiol., 1999, 65, 3742-3745.
• [56] Wolf C.E., Gibbons W.R.: Improved method for quantification of the bacteriocin nisin. J. Appl. Bacteriol., 1996, 80, 453-457.