Equine herpesvirus type 1 quantification in different types of samples by a real-time PCR

• Autorzy: Dzieciatkowski T. , Przybylski M. , Cymerys J. , Turowska A. , Chmielewska A. , Tucholska A. , Banbura M.W.
• Języki publikacji: EN

Abstrakty

EN

Equine herpesvirus type 1 (EHV-1) is one of the major viral agents causing diseases in horses common worldwide. A variety of techniques, including PCR, have been used to diagnose EHV-1 infections. In this paper, an attempt of real-time PCR has been described, which uses specific fluorochrome-labeled TaqMan probes for detection of viral DNA. This method does not require post-amplification manipulations, thereby reducing the risk of cross-contamination. The assay was sensitive enough to detect EHV-1 sequences in different clinical samples, as well in mice neuronal cell cultures. The technique was also very specific – there was no cross reaction with other human and equine herpesviruses. Compared to previously used nested PCR technique, the test was more sensitive and should be useful for the common diagnosis based on its specificity and rapidity.

Słowa kluczowe

EN

equine herpesvirus 1; quantification; real-time polymerase chain reaction; horse; molecular detection; diagnosis; infection; upper respiratory tract; veterinary medicine

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2009
• Tom: 12
• Numer: 3
• Opis fizyczny: p.311-315,fig.,ref.

Twórcy

autor

Dzieciatkowski T.

• Chair and Department of Medical Microbiology, Faculty of Medicine, Medical University of Warsaw, Chalubinskiego 5, 00-004 Warsaw, Poland

autor

Przybylski M.

• Chair and Department of Medical Microbiology, Faculty of Medicine, Medical University of Warsaw, Chalubinskiego 5, 00-004 Warsaw, Poland

autor

Cymerys J.

• Division of Virology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Turowska A.

• Division of Virology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Chmielewska A.

• Division of Virology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Tucholska A.

• Division of Virology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland

autor

Banbura M.W.

• Division of Virology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences-SGGW, Ciszewskiego 8, 02-786 Warsaw, Poland