Technologia DArT – nowe narzędzie do analizy zmienności genetycznej

• Autorzy: Seroczynska A. , Kilian A.

Warianty tytułu

EN

Diversity arrays technology – a new tool for genetic diversity analysis

• Języki publikacji: PL

Abstrakty

PL

Technologia DArT (ang. Diversity Arrays Technology) opracowana z wykorzystaniem technologii mikromacierzy pozwala na jednoczesną analizę wielu sekwencji polimorficznych, których zmienność wynika z różnic genetycznych rozpoznawanych przez enzymy restrykcyjne. Technologię DArT zastosowano dotychczas w badaniach genetycznych 58 gatunków, w tym 53 gatunków roślin, kilku gatunków zwierząt i mikroorganizmów. Umożliwia ona tworzenie profili genetycznych gatunków bez względu na poziom dostępnej informacji molekularnej. Ze względu na automatyzację charakteryzuje się bardzo wysoką wydajnością generowania markerów (kilkaset - kilka tysięcy markerów w jednym eksperymencie), jest tania i wysoce powtarzalna (do 99,8%). Technologia DArT jest przydatna do konstrukcji silnie zagęszczonych map genetycznych w oparciu o same markery DArT lub w połączeniu z innymi typami markerów, do identyfikacji QTL oraz analizy pokrewieństwa genetycznego. Z udziałem markerów DArT skonstruowano mapy molekularne, m.in. dla jęczmienia, pszenicy, sorga, Arabidopsis thaliana, trzciny cukrowej i żyta. Analizowano zmienność genetyczną form w obrębie gatunków strączkowych, pszenicy, jęczmienia, sorga, bananów, eukaliptusa i wielu innych. Technologia DArT jest również wykorzystywana w badaniach mikroorganizmów, w tym w metagenomice oraz w badaniach molekularnych opartych na analizie całego genomu i w poszukiwaniu markerów sprzężonych z ważnymi cechami, przydatnych w hodowli roślin.

EN

Diversity Arrays Technology (DArT) provides a high-throughput whole genome genotyping platform for the detection and scoring of polymorphic loci without any need of prior sequence information. DArT was used in genetic research and breeding of 58 species including 53 plant species, several animals and microorganisms. Thanks to high level of automation in sample processing and data extraction the method is characterized by high efficiency of marker detection (several hundred to many thousands in a single assay), low per marker costs and high repetitiveness (average reproducibility of around 99,8%). The technology is useful for the construction of genetic maps of high density based only on DArT markers or together with other types of molecular markers, for QTL identification and genetic relationship analysis. DArT markers were used for the construction of molecular maps of barley, wheat, sorghum, sugarcane and rye and for genetic diversity analysis of leguminose species, wheat, barley, sorghum, banana, eucaliptus and others. DArT technology is also used in profiling microorganisms (including metagenomics), and molecular breeding based on the analysis of whole genome (genomie selection) and identification markers linked to qualitative characters by BSA method (DArT-BSA).

Słowa kluczowe

PL

genetyka roslin; mapowanie genomu; mapy genetyczne; markery dominujace; markery molekularne; metoda hybrydyzacji; metody molekularne; technika mikromacierzy DNA; technologia DArT; zmiennosc genetyczna

EN

plant genetics; genome mapping; genetic map; dominant marker; molecular marker; hybridization method; molecular method; DArT technology; genetic variability

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2010
• Tom: 555
• Opis fizyczny: s.373-388,rys.,tab.,fot.,bibliogr.

Twórcy

autor

Seroczynska A.

• Katedra Genetyki, Hodowli i Biotechnologii Roślin, Szkoła Główna Gospodarstwa Wiejskiego, ul.Nowoursynowska 159, 02-776 Warszawa

autor

Kilian A.

Bibliografia

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