EN
The aim of investigation was to work out the analytical method of determination of mycotoxins such as: deoxynivalenol, nivalenol and zearlenon in maize grain samples. The analytical procedure consisted of three elementary processes: extraction of analyzed substances from matrix, cleaning of extract using SPE (Solid Phase Extraction) column with C18 active solid and final determination using high performance liquid chromatography with UV detection. The detection limit (LOD) of all tested mycotoxins was 0.002 mg/kg. The range of fortified recoveries of the method was 92–95%.