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2011 | 67 | 02 |

Tytuł artykułu

Badania stanu "niehodowalności" komórek bakterii fermentacji mlekowej w niesprzyjających warunkach rozwoju

Warianty tytułu

EN
Study of the nonculturable state (viable but non-culturable – VBNC) of lactic acid bacteria cells under various adverse growth conditions

Języki publikacji

PL

Abstrakty

EN
The reason for undertaking the research concerning the physiological state of lactic acid bacteria is closely associated with applications of these microorganisms in the food industry. An increased demand referring to microbiological analysis has been affected by the intensification of research assessing the physiological state of microorganisms in unfavorable for them conditions. In technological processes as well as in natural habitats microorganisms can encounter a variety of adverse conditions, arising from the influence of physical and chemical factors such as extremes in temperature, pH or presence of bacteriostatic agents. Therefore, the determination of the viability of bacteria may be challenging due to difficulties in the selection of suitable enumeration methods. The use of conventional methods that rely on the replication and colony formation on growth media is hindered by the decreased reproductive capacity of cells under detrimental conditions. As a result, alternative techniques which do not focus on cell proliferation, but on the metabolic activity or cell structure integrity are being favored. Approaches performing direct detection of different physiological parameters of microbes are connected with the application of modern fluorescence techniques, and in consequence with progress in microbiological research.

Wydawca

-

Rocznik

Tom

67

Numer

02

Opis fizyczny

s.105-109,fot.,bibliogr.

Twórcy

autor
  • Katedra Mikrobiologii Przemysłowej i Żywności, Wydział Nauk o Żywności, Uniwersytet Warmińsko-Mazurski w Olsztynie, Pl.Cieszyński 1, 10-726 Olsztyn

Bibliografia

  • 1.Amann R. I., Zarda B., Stahl D. A., Schleifer K.-H.: Identification of individual prokaryotic cells by using enzyme-labeled, rRNA-targeted oligonucleotide probes. Appl. Environ. Microbiol. 1992, 58, 3007-3011.
  • 2.Auty M. A. E., Gardiner G. E., McBrearty S. J., O’Sullivan E. O., Mulvihill D. M., Collins J. K., Fitzgerald G. F., Stanton C., Ross R. P. M.: Direct in situ viability assessment of bacteria in probiotic dairy products using viability staining in conjunction with confocal scanning laser microscopy. Appl. Environ. Microbiol. 2001, 67, 420-425.
  • 3.Baena-Ruano S., Jimenez-Ot C., Santos-Duenas I., Cantero-Moreno D., Barja F., Garcia-Garcia I.: Rapid method for total, viable and non-viable acetic acid bacteria determination during acetification process. Process Biochemistry 2006, 41, 1160-1164.
  • 4.Barer M. R., Harwood C. R.: Bacterial viability and culturability. Adv. Microb. Physiol. 1999, 41, 93-137.
  • 5.Ben Amor K., Vaughan E. E., de Vos W. M.: Advanced molecular tools for the identification of lactic acid bacteria. J. Nutr. 2007, 137, 741S-747S.
  • 6.Biggerstaff J., Le Puil M., Weidow B., Prater J., Glass K., Radosevich M., White D.: New methodology for viability testing in environmental samples. Mol. Cell. Probes 2006, 20, 141-146.
  • 7.Blasco L., Ferrer S., Pardo I.: Development of specific fluorescent oligonucleotide probes for in situ identification of wine lactic acid bacteria. FEMS Microbiol. Lett. 2003, 225, 115-123.
  • 8.Breeuwand P., Abee T.: Assessment of viability of microorganisms employing fluorescencje techniques. Int. J. Food Microbiol. 2000, 55, 193-200.
  • 9.Bunthof C., Abee T.: Development of a flow cytometric method to analyze subpopulations of bacteria in probiotic products and dairy starters. Appl. Environ. Microbiol. 2002, 68, 2934-2942.
  • 10.Bunthof C., Bloemen K., Breeuwer P., Rombouts F., Abee T.: Flow cytometric assessment of viability of Lactic Acid Bacteria. Appl. Environ. Microbiol. 2001, 67, 2326-2335.
  • 11.Bunthof C., Braak S., Breeuwer P., Rombouts F., Abee T.: Rapid fluorescence assessment of the viability of stressed Lactococcus lactis. Appl. Environ. Microbiol. 1999, 65, 3681-3689.
  • 12.Coeuret V., Dubernet S., Bernardeau M., Gueguen M., Vernoux J. P.: Isolation, characterization and identification of lactobacilli focusing mainly on cheeses and other dairy products. Lait 2003, 83, 269-306.
  • 13.Collado M. C., Moreno Y., Hernandez E., Cobo J. M., Hernandez M.: In vitro viability of Bifidobacterium strains isolated from commercial dairy products exposed to human gastrointestinal conditions. Food Sci. Tech. Int. 2005, 11, 307-314.
  • 14.De Vries M. C., Vaughan E. E., Kleerebezem M., de Vos W. M.: Optimising single cell activity assessment of Lactobacillus plantarum by fluorescent in situ hybridisation as affected by growth. J. Microbiol. Meth. 2004, 59, 109-115.
  • 15.Gatti M., Bernini V., Lazzi C., Neviani E.: Fluorescence microscopy for studying the viability of micro-organisms in natural whey starters. Lett. Appl. Microbiol. 2006, 42, 338-343.
  • 16.Guchte M., Serror P., Chervaux C., Smokvina T., Ehrlich S., Maguin E.: Stress responses in lactic acid bacteria. Anton. Leeuw. 2002, 82, 187-216.
  • 17.Hensiek R., Krupp G., Stackerbrandt E.: Development of diagnosstic oligonucleotide probes for four Lactobacillus species occurring in the intestinal tract. Syst. Appl. Microbiol. 1992, 15, 123-128.
  • 18.Hermida M., Taboada M., Menendes S., Rodriguez-Otero J. L.: Semi-automated direct epifluorescent filter technique for total bacterial count in raw milk. J. AOAC Int. 2000, 83, 1345-1348.
  • 19.Joux F., Lebaron F.: Use of fluorescent probes to assess physiological functions of bacteria at single-cell level. Microbes Infection 2000, 2, 1523-1535.
  • 20.Lahtinen S., Gueimonde M., Ouwehand A., Reinikainen J., Salminen S.: Comparison of methods to enumerate probiotic bifidobacteria in a fermented food product. Food Microbiol. 2006, 23, 571-577.
  • 21.Lahtinen S., Gueimonde M., Ouwehand A., Reinikainen J., Salminen S.: Probiotic bacteria may become dormant during storage. Appl. Environ. Microbiol. 2005, 71, 1662-1663.
  • 22.Łaniewska-Trokenheim Ł., Olszewska M., Mikš-Krajnik M., Zadernowska A.: Patterns of survival and volatile metabolites of selected Lactobacillus strains in long-term incubation in milk. J. Microbiol. 2010, 48, 445-451.
  • 23.Mikš M., Warmiñska-Radyko I.: Wybrane techniki fluorescencyjne w badaniach stanu fizjologicznego i przeżywalności komórek bakteryjnych w żywności. Medycyna Wet. 2008, 64, 623-628.
  • 24.Moreno Y., Collado M., Ferrus M., Cobo J., Hernandez E., Hernandez M.: Viability assessment of lactic acid bacteria in commercial dairy products stored at 4°C using LIVE/DEAD BacLight staining and conventional plate counts. Int. J. Food Sci. Tech. 2006, 41, 275-280.
  • 25.Morono Y., Takano S., Miyanaga K., Tanji Y., Unno&Katsutoshi H.: Application of glutaraaldehyde for the staining of esterase-active cells with carboxyfluorescein diacetate. Biotechnol. Lett. 2004, 26, 379-383.
  • 26.Papadimitriou K., Pratsinis H., Nebe-Von-Caron G., Kletsas D., Tsakalidou E.: Rapid assessment of the physiological status of Streptococcus macedonicus by flow cytometry and fluorescence probes. Int. J. Food Microbiol. 2006, 111, 197-205.
  • 27.Paszyńska-Wesołowska I., Bartoszcze M.: Bakterie w stadium VBNC - zagrożenie dla zdrowia człowieka. Medycyna Wet. 2009, 65, 228-231.
  • 28.Rault A., Beal C., Ghorbal S., Ogier J.-C., Bouix M.: Multiparametric flow cytometry allows rapid assessment and comparison of lactic acid bacteria viability after freezing and during frozen storage. Cryobiology 2007, 55, 35-43.
  • 29.Roszak D. B., Grimes D. J., Colwell R. R.: Viable but non-recoverable stage of Salmonella enteritidis in aquatic system. Can. J. Microbiol. 1984, 30, 334-338.
  • 30.Vogel R., Bocker G., Stolz P., Ehrmann M., Fanta D., Ludwig W., Pot B., Kersters K., Schleifer K. H., Hammes W.: Identification of lactobacilli from sourdough and description of Lactobacillus pontis sp. nov. Int. J. Syst. Bacteriol. 1994, 44, 223-239.
  • 31.Wallner G., Amann R., Beisker W.: Optimizing fluorescent in situ hybridisation with rRNA-targeted oligonucleotide probes for flow cytometric identification of microorganisms. Cytometry 1993, 14, 136-143.
  • 32.Warmińska-Radyko I., Olszewska M., Mikš-Krajnik M.: Effect of temperature and sodium chloride on the growth and metabolism of Lactococcus strains in long-term incubation of milk. Milchwissenschaft 2010, 65, 32-35.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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