The adipose tissue gene expression in mice with different nitric oxide availability

• Autorzy: Razny U. , Kiec-Wilk B. , Polus A. , Wator L. , Dyduch G. , Partyka L. , Bodzioch M. , Tomaszewska R. , Wybranska I.
• Języki publikacji: EN

Abstrakty

EN

Mice with the knockout of endothelial nitric oxide synthase (eNOS ko) demonstrate symptoms resembling the human metabolic syndrome. NO has been recently demonstrated to be deeply involved in regulation of not only blood flow and angiogenesis, but also in modulation of mammalian basal energy substrate metabolism. Asymmetric dimethylarginine (ADMA) is an endogenous competitive inhibitor of NOS. The enzyme dimethylarginine dimethylaminohydrolase (DDAH) catabolizes ADMA, what leads to increase of endogenous NO bioavailability. This study was aimed to compare the brown (BAT) and white (WAT) adipose tissue gene expression of age matched mice with decreased (eNOS ko) and increased (overexpressing DDAH) endogenous NO generation. The 19 week old eNOS ko mice demonstrated significantly lower weight, higher circulating glucose, insulin, leptin and cholesterol concentrations. The adiponectin as well as fasting triglyceride concentrations were not significantly altered. Animals with DDAH knock in, presented significantly increased angiogenic activity than eNOS ko mice. The microarray analysis pointed to activation of adipogenesis-related genes in eNOS ko mice in WAT, what was in contrast with the inhibition observed in the DDAH overexpressing mice. The angiogenesis related gene expression was down-regulated in both models in comparison to WT animals. This study support the multipotential role of endogenous NO in maintaining homeostasis of energy substrate catabolism.

Słowa kluczowe

EN

adipose tissue; angiogenesis; asymmetric dimethylarginine; blood flow; dyslipidemia; endothelial nitric oxide synthase; gene expression; mice; microarray; mouse; nitric oxide; obesity

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 2010
• Tom: 61
• Numer: 5
• Opis fizyczny: p.607-618,fig.,ref.

Twórcy

autor

Razny U.

• Jagiellonian University Medical College; 15a Kopernika Street, 31-501 Krakow, Poland

autor

Kiec-Wilk B.

autor

Polus A.

autor

Wator L.

autor

Dyduch G.

autor

Partyka L.

autor

Bodzioch M.

autor

Tomaszewska R.

autor

Wybranska I.

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