Wpływ nanocząsteczek srebra na żywotność i aktywność proliferacyjną jednojądrzastych leukocytów krwi obwodowej i splenocytów mysich - badania in vitro

• Autorzy: Malaczewska J.

Warianty tytułu

EN

In vitro effect of silver nanoparticles on the viability and proliferative response of mice peripheral blood mononuclear cells and splenocytes

• Języki publikacji: PL

Abstrakty

EN

Silver nanoparticles (SNP) have been recently one of the most widely utilized nanomaterials, mostly because of their antimicrobial activity. For some time there has been a great interest in SNP of unconventional medicine, which recommends their use not only as an antimicrobial, but also as an immunostimulant. However, little is known about SNP’s impact on immunocompetent cells in vitro. The aim of a present study was to investigate the influence of the colloidal nano-silver solution on the viability and proliferative response of mice peripheral blood mononuclear leukocytes and splenocytes in vitro. After isolation cells were cultured in complete RPMI-1640 medium containing 0 (control), 20, 10, 5, 2, 1, 0.5, 0.2, 0.1 and 0.05 ppm of SNP, for 24 and 48 h to investigate SNP’s impact on cell viability, and for 72 h to evaluate their effect on the proliferative response of cells. Both parameters were assessed using MTT assay. Obtained results suggest that SNP have significant influence on the both investigated parameters. High doses of SNP significantly decreased (p < 0.01) the viability (concentrations of 5-20 ppm for splenocytes and 2-20 ppm for leukocytes, respectively) and proliferative response of cells (splenocytes: 5-20 ppm, leukocytes: 10-20 ppm) whereas low SNP’s doses slightly increased (p > 0.05) the viability of cells (splenocytes: 1 ppm, leukocytes: 0.2 ppm) and significantly increased stimulation index of both cell types induced by mitogens (splenocytes: 0.2-0.5 ppm, p < 0.05; leukocytes: 0.1-0.5 ppm, p < 0.01). These experimental data constitute an encouragement for further investigations concerning the possibility of therapeutic use of SNP’s low doses.

Słowa kluczowe

PL

aktywnosc proliferacyjna; badania in vitro; leukocyty; leukocyty jednojadrzaste; myszy; nanoczasteczki; splenocyty; srebro; zywotnosc

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2010
• Tom: 66
• Numer: 12
• Opis fizyczny: s.847-851,tab.,bibliogr.

Twórcy

autor

Malaczewska J.

• Katedra Mikrobiologii i Immunologii Klinicznej, Wydział Medycyny Weterynaryjnej, Uniwersytet Warmińsko-Mazurski w Olsztynie, ul.Oczapowskiego 13, 10-718 Olsztyn

Bibliografia

• 1. Bhol K. C., Schechter P. J.: Topical nanocrystalline silver cream suppresses inflammatory cytokines and induces apoptosis of inflammatory cells in a murine model of allergic contact dermatitis. Br. J. Dermatol. 2005, 152, 1235-1242.
• 2. Foldbjerg R., Olsen P., Hougaard M., Dang D. A., Hoffmann H. J., Autrup H.: PVP-coated silver nanoparticles and silver ions induced reactive oxygen species, apoptosis and necrosis in THP-1 monocytes. Toxicol. Lett. 2009, 190, 156-162.
• 3. Lubick N.: Nanosilver toxicity: ions, nanoparticles - or both? Environ. Sci. Technol. 2008, 42, 8617.
• 4. Luoma S. N.: Silver nanotechnologies and the environment: old problems or new challenges? PEN 15, september 2008 (report by Project on Emerging Nanotechnologies; http://www.nanotechproject.org/publications/).
• 5. Mossmann T.: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 1983, 65, 55-63.
• 6. Nadworny P. L., Wang J. F., Tredget E. E., Burrell R. E.: Anti-inflammatory activity of nanocrystalline silver in a porcine contact dermatitis model. Nanomed. Nanotechnol. Biol. Med. 2008, 4, 241-251.
• 7. Park E. J., Yi J., Kim Y., Choi K., Park K.: Silver nanoparticles induce cytotoxicity by a Trojan-horse type mechanism. Toxicol. in Vitro 2010, 24, 872-878.
• 8. Santoro C. M., Duchsherer N. L., Grainger D. W.: Minimal in vitro anti-microbial efficacy and ocular cell toxicity from silver nanoparticles. Nanobiotechnol. 2007, 3, 55-65.
• 9. Shin S. H., Ye M. K., Kim H. S., Kang H. S.: The effects of nano-silver on the proliferation and cytokine expression by peripheral blood mononuclear cells. Int. Immunopharmacol. 2007, 7, 1813-1818.
• 10. Tian J., Wong K. K. Y., Ho C. M., Lok C. N., Yu W. Y., Che C. M., Chiu J. F., Tam P. K. H.: Topical delivery of silver nanoparticles promotes wound healing. ChemMedChem 2007, 2, 129-136.
• 11. Yen H. J., Hsu S. H., Tsai C. L.: Cytotoxicity and immunological response of gold and silver nanoparticles of different sizes. Small 2009, 5, 1553-1561.