Evaluation of PCR test for detection of dermonecrotoxin of Bordetella bronchiseptica

• Autorzy: Stepniewska K. , Markowska-Daniel I.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was the evaluation and optimisation of PCR test for the detection of dermonecrotoxin gene (DNT) of Bordetella bronchiseptica. For the optimisation of the test, vaccine strain B16 was used. The optimisation procedure included: estimation of optimal Mg²⁺ concentration, annealing temperature, numbers of cycles, as well as sensitivity. The specificity of PCR test was checked with DNA of other pathogens existing in pigs' respiratory tract. The elaborated test was specific and sensitive to detect DNT gene of B. bronchiseptica, in both clinical samples, as well as in pure culture of the bacteria.

Słowa kluczowe

EN

polymerase chain reaction; detection; dermonecrotoxin gene; Bordetella bronchiseptica; etiological agent; atrophic rhinitis; pig; economic loss; animal farm; animal disease

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2010
• Tom: 54
• Numer: 4
• Opis fizyczny: p. 495-499,fig.,ref.

Twórcy

autor

Stepniewska K.

• Department of Swine Disease, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Markowska-Daniel I.

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