Semen quality assessments and their significance in reproductive technology

• Autorzy: Kordan W. , Fraser L. , Wysocki P. , Strzezek R. , Lecewicz M. , Mogielnicka-Brzozowska M. , Dziekonska A. , Soliwoda D. , Koziorowska-Gilun M.
• Języki publikacji: EN

Abstrakty

EN

Semen quality assessment methods are very important in predicting the fertilizing ability of persevered spermatozoa and to improve animal reproductive technology. This review discusses some of the current laboratory methods used for semen quality assessments, with references to their relevance in the evaluation of male fertility and semen preservation technologies. Semen quality assessment methods include sperm motility evaluations, analyzed with the computer-assisted semen analysis (CASA) system, and plasma membrane integrity evaluations using fluorescent stains, such as Hoechst 33258 (H33258), SYBR-14, propidium iodide (PI), ethidium homodimer (EthD) and 6-carboxyfluorescein diacetate (CFDA), and biochemical tests, such as the measurement of malondialdehyde (MDA) level. This review addresses the significance of specific fluorochromes and ATP measurements for the evaluation of the sperm mitochondrial status. Laboratory methods used for the evaluation of chromatin status, DNA integrity, and apoptotic changes in spermatozoa have been discussed. Special emphasis has been focused on the application of proteomic techniques, such as two-dimensional (2-D) gel electrophoresis and liquid chromatography mass spectrometry (LC-MS/MS), for the identification of the properties and functions of seminal plasma proteins in order to define their role in the fertilization-related processes.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2013
• Tom: 16
• Numer: 4
• Opis fizyczny: p.823-833,fig.,ref.

Twórcy

autor

Kordan W.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Fraser L.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Wysocki P.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Strzezek R.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Lecewicz M.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Mogielnicka-Brzozowska M.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Dziekonska A.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Soliwoda D.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

autor

Koziorowska-Gilun M.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn, Poland

Bibliografia

• Aitken RJ, Wingate JK, De Iuliis GN, McLaughlin EA (2007) Analysis of lipid peroxidation in human spermatozoa using BODIPY C11. Mol Hum Reprod 13: 203-211.
• Antończyk A, Niżański W, Twardoń J, Kozdrowski R, Ochota M, Błasiak K, Mikołajewska N, Stańczyk E (2010) Current views on computer assisted sperm analysis. Medycyna Weterynaryjna 66: 663-667.
• Anzar M, He L, Buhr MM, Kroetsch TG, Pauls KP (2002) Sperm apoptosis in fresh and cryopreserved bull semen detected by flow cytometry and its relationship with fertility. Biol Reprod 66: 354-360.
• Balhorn R (1982) A model for the structure of chromatin in mammalian sperm. J Cell Biol 93: 298-305.
• Ball BA, Gravance CG, Medina V, Baumber J, Liu IKM (2000) Catalase activity in equine semen. Am J Vet Res 61: 1026-1030.
• Ball BA, Vo A (2002) Detection of lipid peroxidation in equine spermatozoa based upon the lipophilic fluorescent dye C11-BODIPY581/591. J Androl 23: 259-269.
• Brandon CI, Heusner GL, Caudle AB, Fayrer-Hosken RA (1999) Two-dimensional polyacrylamide gel electrophoresis of equine seminal plasma proteins and their correlation with fertility. Theriogenology 52: 863-873.
• Broekhuijse MLWJ, Šoštarić E, Feitsma H, Gadella BM (2011) Additional value of computer assisted semen analysis (CASA) compared to conventional motility assessments in pig artificial insemination. Theriogenology 76: 1473-1486.
• Ciereszko A, Glogowski J, Demianowicz W, Strzeżek J (1994) Stimulation of aspartate aminotransferase from farm animal semen by piridoxal 5’-phosphate. Anim Reprod Sci 34: 327-341.
• Ciereszko A, Glogowski J, Strzeżek J, Demianowicz W (1992) Low stability of aspartate aminotransferase activity in boar semen. Theriogenology 37: 1269-1281.
• Cremades T, Roca J, Rodríguez-Martínez H, Abaigar T, Vazquez JM, Martinez EA (2005) Kinematic changes during the cryopreservation of boar spermatozoa. J Androl 26: 610-618.
• Dart MG, Mesta J, Creshaw C, Ericsson SA (1994) Modified resazurin test for determining the fertility potential of bovine spermatozoa. Arch Androl 33: 71-75.
• de Jonge CJ, Tarchala SM, Rawlings R G, Binor Z, Radwańska E (1993) Acrosin activity in human spermatozoa in relation to semen quality and in vitro fertilization. Hum Reprod 8: 253-257.
• du Plessis SS, Kashou AH, Benjamin DJ, Yadav SP, Agarwal A (2011) Proteomics: a subcellular look at spermatozoa. Reprod Biol Endocrinol 9: 36.
• Dziekońska A, Fraser L, Strzeżek J (2009) Effect of different storage temperatures on the metabolic activity of spermatozoa following liquid storage of boar semen. J Anim Feed Sci 18: 638-649.
• Esch FS, Ling NC, Bohlen P, Ying SY, Guillemin R (1983) Primary structure of PDC-109, a major protein constituent of bovine seminal plasma. Biochem Biophys Res Commun 113: 861-867.
• Evenson DP, Thompson L, Jost L (1994) Flow cytometric evaluation of boar semen by the sperm chromatin structure assay as related to cryopreservation and fertility. Theriogenology 41: 637-651.
• Evenson DP, Larson KL, Jost LK (2002) Sperm chromatin structure assay: its clinical use for detecting sperm DNA fragmentation in male infertility and comparisons with other techniques. J Androl 23: 25-43.
• Evenson DP, Wixon R (2008) Data analysis of two in vivo fertility studies using sperm chromatin structure assay-derived DNA fragmentation index vs. pregnancy outcome. Fertil Steril 90: 1229-1331.
• Fraser L (2004) Structural damage to nuclear DNA in mammalian spermatozoa: its evaluation techniques and relationship with male infertility. Pol J Vet Sci 7: 311-321.
• Fraser L, Dziekońska A, Strzeżek R, Strzeżek J (2007) Dialysis of boar semen prior to freezing-thawing: its effects on post-thaw sperm characteristics. Theriogenology 67: 994-1003.
• Fraser L, Gorszczaruk K, Strzeżek J (2001) Relationship between motility and membrane integrity of boar spermatozoa in media varying in osmolality. Reprod Domest Anim 36: 325-329.
• Fraser L, Parda A, Filipowicz K, Strzeżek J (2010) Comparison of post-thaw DNA integrity of boar spermatozoa assessed with the neutral Comet assay and sperm-Sus Halomax test kit. Reprod Domest Anim 45: e155-e160.
• Fraser L, Strzeżek J (2004) The use of comet assay to assess DNA integrity of boar spermatozoa following liquid preservation at 5°C and 16°C. Folia Histochem Cytobiol 42: 49-55.
• Fraser L, Strzeżek J (2007a) Effect of different procedures of ejaculate collection, extenders and packages on DNA integrity of boar spermatozoa following freezing-thawing. Anim Reprod Sci 99: 317-329.
• Fraser L, Strzeżek J (2007b) Is there a relationship between the chromatin status and DNA fragmentation of boar spermatozoa following freezing-thawing? Theriogenology 68: 248-257.
• Fraser L, Strzeżek J, Kordan W (2011a) Effect of freezing on sperm nuclear DNA. Reprod Domest Anim 46 (Suppl. 2): 14-17.
• Fraser L, Zasiadczyk Ł, Lecewicz M, Kordan W (2011b) Comparative study of different methods for plasma membrane integrity assessment of frozen-thawed boar spermatozoa. Bull Vet Inst Pulawy 55: 231-236.
• Gadea J (2005) Sperm factors related to in vitro and in vivo porcine fertility. Theriogenology 63: 431-444.
• Garner DL, Johnson LA (1995) Viability assessment of mammalian sperm using SYBR-14 and propidium iodide. Biol Reprod 53: 276-284.
• Garner DL, Pinkel D, Johnson LA, Pace MM (1986) Assessment of spermatozoal function using dual fluorescent staining and flow cytometric analyses. Biol Reprod 34: 127-138.
• Garner DL, Thomas CA, Joerg HW, DeJarnette JM, Marshall CE (1997) Fluorometric assessments of mitochondrial function and viability in cryopreserved bovine spermatozoa. Biol Reprod 57: 1401-1406.
• Gączarzewicz D, Piasecka M, Udała J, Błaszczyk B, Stankiewicz T, Laszczyńska M (2010) Plasma membrane changes during the liquid storage of boar spermatozoa: a comparison of methods. Acta Vet Hung 58: 105-116.
• Gerena RL, Irikura D, Urade Y, Eguchi N, Chapman DA, Killian GJ (1998) Identification of a fertility-associated protein in bull seminal plasma as lipocalin-type prostaglandin D synthase. Biol Reprod 58: 826-833.
• Glogowski J, Strzeżek J, Jazdżewski J (1994) Intensity of 3H-actinomycin (3H-AMD) binding to chromatin of bull spermatozoa. Reprod Domest Anim 29: 396-403.
• Gogol P, Wierzchoś-Hilczer A, Cegła M (2007) Iron-induced luminescence as a method for assessing lipid peroxidation of frozen-thawed goat spermatozoa. Animal 1: 844-848.
• Holt C, Holt WV, Moore HD, Reed HC, Curnock RM (1997) Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations: results of two fertility trials. J Androl 18: 312-323.
• Hirano Y, Shibahara H, Obara H, Suzuki T, Takamizawa S, Yamaguchi C, Tsunoda H, Sato I (2001) Relationships between sperm motility characteristics assessed by the computer-aided sperm analysis (CASA) and fertilization rates in vitro. J Assist Reprod Genet 18: 213-218.
• Idziorek T, Estaquier J, De Bels F, Ameisen JC (1995) YOPRO-1 permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability. J Immunol Methods 185: 249-258.
• Januskauska A, Johannisson A, Rodríguez-Martínez H (2001) Assessment of sperm quality through fluorometry and sperm chromatin structure assay in relation to field fertility of frozen-thawed semen from Swedish AI bulls. Theriogenology 55: 947-961.
• Jeyendran RS, van der Ven HH, Perez-Pelaez M, Crabo BG, Zaneveld LJ (1984) Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J Reprod Fertil 70: 219-228.
• Johnson LA, Weitze KF, Fiser P, Maxwell WMC (2000) Storage of boar semen. Anim Reprod Sci 62: 143-172.
• Kemme M, Madiraju MV, Krauhs E, Zimmer M, Scheit KH (1986) The major protein of bull seminal plasma is a secretory product of seminal vesicle. Biochim Biophys Acta 884: 282-290.
• Killian GJ, Chapman DA, Rogowski LA (1993) Fertility-associated proteins in Holstein bull seminal plasma. Biol Reprod 49: 1202-1207.
• Kordan W, Lecewicz M, Strzeżek R, Dziekońska A, Fraser L (2010) Effect of platelet activating factor (PAF) supplementation in semen extender on viability ant ATP content of cryopreserved canine spermatozoa. Pol J Vet Sci 13: 571-579.
• Kordan W, Lecewicz M, Tobolski J (2009a) Effect of platelet-activating factor on motility, plasmalemma integrity, the process of capacitation and acrosome reaction of fresh and cryopreserved boar spermatozoa. Pol J Vet Sci 12: 175-181.
• Kordan W, Malinowska A, Mogielnicka M, Lecewicz M (2009b) Application of mass spectrometry (MS) in the structural analysis of selected polypeptides of boar seminal plasma obtained after two-dimensional electrophoresis (2-D PAGE). Animal Science Papers and Reports 27: 41-50.
• Kordan W, Strzeżek J (2002) Effect of platelet-activating factor on motility parameters and plasmalemma integrity of boar spermatozoa. Animal Science Papers and Reports 20: 37-45.
• Kordan W, Strzeżek J (2006) Synthetic platelet activating factor (PAF) as a boar semen extender component. Medycyna Weterynaryjna 62: 560-562.
• Kordan W, Strzeżek J, Soliwoda D, Lecewicz M, Mogielnicka M (2008) Mapping of boar seminal plasma proteins by two-dimensional polyacrylamide gel electrophoresis. Medycyna Weterynaryjna 64: 223-226.
• Kowalówka M, Wysocki P, Fraser L, Strzeżek J (2008) Extracellular superoxide dismutase of boar seminal plasma. Reprod Domest Anim 43: 490-496.
• Koziorowska-Gilun M, Koziorowski M, Fraser L, Strzeżek J (2010) Antioxidant defence system of boar cauda epididymidal spermatozoa and reproductive tract fluids. Reprod Domest Anim 46: 527-533.
• Linfor JJ, Meyers SA (2002) Detection of DNA damage in response to cooling injury in equine spermatozoa using single-cell electrophoresis. J Androl 23: 107-113.
• López-Fernández C, Fernández JL, Gósalbez A, Arroyo F, Vázquez JM, Holt WV, Gosálvez J (2008) Dynamics of sperm DNA fragmentation in domestic animals III. Ram. Theriogenology 70: 898-908.
• Manjunath P, Sairam MR (1987) Purification and biochemical characterization of three major acidic proteins (BSP-A1, BSP-A2 and BSP-A3) from bovine seminal plasma. Biochem J 241: 685-692.
• Mortimer ST (1997) A critical review of the physiological importance and analysis of sperm movement in mammals. Hum Reprod Update 3: 403-439
• Mukhopadhyay CS, Gupta AK, Yadav BR, Chauhan IS, Gupta A, Mohanty TK, Raina VS (2011) Effect of cryopreservation on sperm chromatin integrity and fertilizing potential in bovine semen. Livest Sci 136: 114-121.
• Panisko EA, Conrads TP, Goshe MB, Veenstra TD (2002) The postgenomic age: characterization of proteomes. Exp Hematol 30: 97-107.
• Parks JE, Lynch DV (1992) Lipid composition and thermotropic phase behavior of boar, bull, stallion, and rooster sperm membranes. Cryobiology 29: 255-266.
• Partyka A, Niżański W, Ochota M (2012) Methods of assessment of cryopreserved semen. In: Katkov II (ed) Current Frontiers in Cryobiology. InTech Open Access Publisher, Rijeka, Croatia, pp 547-574.
• Peña FJ, Johannisson A,Wallgren M, Rodríguez-Martínez H (2003) Assessment of fresh and frozen-thawed boar semen using an Annexin-V assay: a new method to evaluate sperm membrane integrity. Theriogenology 60: 677-689.
• Peña FJ, Saravia F, Johannisson A, Walgren M, Rodríguez-Martínez H (2005) A new and simple method to evaluate early membrane changes in frozen-thawed boar spermatozoa. Int J Androl 28: 107-114.
• Peris SI, Moriier A, Dufour M, Bailey JL (2004) Cryopreservation of ram semen facilitates sperm DNA damage: relationship between sperm andrological parameters and the sperm chromatin structure assay. J Androl 25: 224-233.
• Pinto CR, Kozink DM (2008) Simplified hypoosmotic swelling testing (HOST) of fresh and frozen-thawed canine spermatozoa. Anim Reprod Sci 104: 450-455.
• Rodríguez-Martínez H (2003) Laboratory semen assessment and prediction of fertility: stillutopia? Reprod Domest Anim 38: 312-318.
• Rodríguez-Martínez H, Kvist U, Ernerudh J, Sanz L, Calvete JJ (2011) Seminal plasma proteins: what role do they play? Am J Reprod Immunol 66 (Suppl 1): 11-22.
• Schilling E, Vengust M (1987) Frequency of semen collection in boars and quality of ejaculates as evaluated by the osmotic resistance of acrosomal membranes. Anim Reprod Sci 12: 283-290.
• Sikka SC (2004) Role of oxidative stress and antioxidants in andrology and assisted reproductive technology. J Androl 25: 5-18.
• Silva PF, Gadella BM (2006) Detection of damage in mammalian sperm cells. Theriogenology 65: 958-978.
• Strzeżek J, Fraser L, Demianowicz W, Kordan W, Wysocki P, Hołody D (2000) Effect of depletion tests (DT) on the composition of boar semen. Theriogenology 54: 949-963.
• Strzeżek J, Fraser L, Kuklińska M, Dziekońska A, Lecewicz M (2004) Effects of dietary supplementation with polyunsaturated fatty acids and antioxidants on biochemical characteristics of boar semen. Reprod Biol 4: 271-287.
• Strzeżek J, Kordan W (2003) Effects of decondensing agents on chromatin stability of boar spermatozoa – radioisotope study. Animal Science Papers and Reports 21: 167-181.
• Strzeżek J, Kordan W, Glogowski J, Wysocki P, Borkowski K (1995) Influence of semen-collection frequency on sperm quality in boars, with special reference to biochemical markers. Reprod Domest Anim 30: 85-94.
• Strzeżek J, Łapkiewicz S, Lecewicz M (1999) A note on antioxidant capacity of boar seminal plasma. Animal Science Papers and Reports 17: 181-188.
• Strzeżek J, Torska J (2001) Activity of proteinase inhibitors seminal plasma of vasectomised boars. Animal Science Papers and Reports 19: 111-121.
• Strzeżek J, Wysocki P, Kordan W, Kuklińska M, Mogielnicka M, Soliwoda D, Fraser L (2005) Proteomics of boar seminal plasma – current studies and possibility of their application in biotechnology of animal reproduction. Reprod Biol 5: 279-290.
• Strzeżek R, Koziorowska-Gilun M, Kowalówka M, Strzeżek J (2009) Characteristics of antioxidant system in dog semen. Pol J Vet Sci 12: 55-60.
• Torska J, Strzeżek J (1985) Activity of acrosin inhibitors in bull seminal plasma in an annual cycle. Comp Biochem Phys B 81: 519-522.
• Trzcińska M, Bryła M, Smorąg Z (2010) Ocena jakości nasienia transgenicznych knurów przy zastosowaniu markerów apoptotycznych. Przegląd Hodowlany 12: 1-3.
• Vijayaraghavan S, Manjunath P, Hoskins DP (1989) The seminal vesicle protein BSP A1/A2 binds to bovine epididymal sperm and inhibits calcium uptake. Biol Reprod 40 (suppl 1): 142.
• Wysocki P, Koncicka K, Strzeżek J (2009) Is the phosphorylation status of tyrosine proteins a marker for the cryo-capacitation of boar spermatozoa? Bull Vet Inst Pulawy 53: 229-232.
• Wysocki P, Strzezek J (2003) Purification and characterization of a protein tyrosine acid phosphatase from boar seminal vesicle glands. Theriogenology 59: 1011-1025.
• Wysocki P, Strzeżek J (2006) Isolation and biochemical characteristics of a molecular form of epididymal acid phosphatase of boar seminal plasma. Theriogenology 66: 2152-2159.
• Yanagida M (2002) Functional proteomics: current achievements. J Chromatogr B 771: 89-106.
• Yeste M, Briz M, Pinart E, Sancho S, Garcia-Gil N, Badia E, Bassols J, Pruneda A, Bussalleu E, Casas I, Bonet S (2008) Boar spermatozoa and prostaglandin F2α: Quality of boar sperm after the addition of prostaglandin F2α to the short-term extender over cooling time. Anim Reprod Sci 108: 180-195
• Yousef GM, Diamandis M, Jung K, Diamandis EP (2001) Molecular cloning of a novel human acid phosphatase gene (ACPT) that is highly expressed in the testis. Genomics 74: 385-395.
• Zrimšek P, Kurc J, Kosec M, Mrkun J (2004) Spectrophotometric application of resazurin reduction assay to evaluate boar semen quality. Int J Androl 27: 57-62.