Interactions between plant-derived oestrogenic substances and the mycoestrogen zearalenone in a bioassay with MCF-7 cells

• Autorzy: Hessenberger S. , Botzi K. , Degrassi C. , Kovalsky P. , Schwab C. , Schatzmayr D. , Schatzmayr G. , Fink-Gremmels J.
• Języki publikacji: EN

Abstrakty

EN

Human and animal diets may contain several non-steroidal oestrogenic compounds which originate either from plants (phytoestrogens) or from fungi that infect plants (mycoestrogens such as zearalenone (ZEN)). Phytoestrogens may compete with ZEN in binding to the oestrogen receptor β and thereby may counteract the oestrogenic activity of ZEN. Using a modified version of the E-screen assay, plant-derived oestrogenic substances were tested for their proliferative or anti-proliferative effect on oestrogen-dependent MCF-7 cells. The samples were additionally tested for their ability to influence the oestrogenic activity of ZEN (1 μM). Among the individual substances tested, 8-prenylnaringenin had the strongest effect, as cell proliferation was increased by 78% at the lowest concentration (0.23 μM), and by 167% at the highest concentration (29.4 μM). Coumestrol (5.83 μM) increased cell proliferation by 39%, and genistein (370 μM) by 61%, respectively. Xanthohumol and enterolactone did not stimulate cell proliferation significantly. In the co-incubation experiments with ZEN, none of the single substances was able to decrease the oestrogenic activity of ZEN. Only for 8-prenylnaringenin (14.7 and 29.4 μM) was a trend towards an increase in the ZEN-induced cell proliferation up to 72% observed. In conclusion, with the exception of 8-prenylnaringenin, no substantial interaction between phytoestrogens and the mycotoxin ZEN could be detected using a bioassays with MCF-7 cells.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2017
• Tom: 20
• Numer: 3
• Opis fizyczny: p.513-520,fig.,ref.

Twórcy

autor

Hessenberger S.

• Biomin Research Center, Technopark 1, 3430 Tulln, Austria

autor

Botzi K.

• Biomin Research Center, Technopark 1, 3430 Tulln, Austria

autor

Degrassi C.

• Biomin Research Center, Technopark 1, 3430 Tulln, Austria

autor

Kovalsky P.

• Biomin Holding GmbH, Erber Campus 1, 3131 Getzersdorf, Austria

autor

Schwab C.

• Biomin Holding GmbH, Erber Campus 1, 3131 Getzersdorf, Austria

autor

Schatzmayr D.

• Biomin Research Center, Technopark 1, 3430 Tulln, Austria

autor

Schatzmayr G.

• Biomin Research Center, Technopark 1, 3430 Tulln, Austria

autor

Fink-Gremmels J.

• Institute for Risk Assessment Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 104, 3584 CM Utrecht, The Netherlands

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Identyfikatory

DOI

10.1515/pjvs-2017-0062