Identification of methanotrophic bacteria community in the Jastrzebie-Moszczenica coal mine by fluorescence in situ hybridization and PCR techniques

• Autorzy: Wolinska A. , Pytlak A. , Stepniewska Z. , Kuzniar A. , Piasecki C.
• Języki publikacji: EN

Abstrakty

EN

The aim of our study was to identify the methanotrophic group of bacteria inhabiting coal mine rocks at the Jastrzębie-Moszczenica (Jas-Mos) coal mine, by application of molecular techniques: polymerase chain reaction (PCR) and fluorescence in situ hybridization (FISH). In the first stage of the experiment, methanotrophic activity (AM) of the rock material at different temperatures (20 and 30ºC) and methane (CH₄) concentrations (1, 5, 10, 20%) was determined. AM noted both in 20 and 30ºC reached a similar level equaling c.a. 0.2 µM CH₄ g⁻¹·day⁻¹. The PCR reactions were performed using primers specific for pmoA gene (encoding a key subunit of particulate methane monooxygenase) – A189f and mb661, whereas FISH was realized with Mg705, Mg84 (Type I of methanotrophs), and Ma450 (Type II of methanotrophs) probes. Sequence analysis of pmoA gene demonstrated that microorganisms, being present in the investigated rocks material were similar with 95-100% identity to the following methanotrophs genera: Methylosinus, Methylobacter, Methylocystis. Concentrations of CH₄ between 1-10% did not affect on bacterial variability, but in combination with 20% of CH₄, significant dominance of methanotrophic bacteria Type I was stated.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2013
• Tom: 22
• Numer: 1
• Opis fizyczny: p.275-282,fig.,ref.

Twórcy

autor

Wolinska A.

• Institute of Biotechnology, Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Konstantynów 1 I, 20-708 Lublin, Poland

autor

Pytlak A.

• Institute of Biotechnology, Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Konstantynów 1 I, 20-708 Lublin, Poland

autor

Stepniewska Z.

• Institute of Biotechnology, Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Konstantynów 1 I, 20-708 Lublin, Poland

autor

Kuzniar A.

• Institute of Biotechnology, Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Konstantynów 1 I, 20-708 Lublin, Poland

autor

Piasecki C.

• Institute of Biotechnology, Department of Biochemistry and Environmental Chemistry, The John Paul II Catholic University of Lublin, Konstantynów 1 I, 20-708 Lublin, Poland

Bibliografia

• 1. McDONALD I.R., BODROSSY L., CHEN Y., MURRELL J.C. Molecular ecology technique for the study of aerobic methanotrophs. Appl. Microbiol. Environ. 74, 1305, 2008.
• 2. COSTELLO A.M., AUMAN A.J., MACALADY J.L., SCOW K.M., LIDSTROM M.E. Estimation of methanotroph abundance in a freshwater sediment. Environ. Microbiol. 4, 443, 2002.
• 3. HANSON R.S., HANSON T.E. Methanotrophic bacteria. Microbiol. Rev. 60, 439, 1996.
• 4. HAN B., CHEN Y., ABELL G., JIANG H., BODROSSY L., ZHAO J., MURRELL J.C., XING X.H. Diversity and activity of methanotrophs in alkaline soil from a Chinese coal mine. Microbiol. Ecol. 70, 196, 2009.
• 5. TROTSENKO Y.A., MURRELL J.C. metabolic aspects of aerobic obligate methanotrophy. Adv. Appl. Microbiol. 63, 183, 2008.
• 6. THEISEN A.R., ALI M.H., RADAJEWSKI S., DUMONT M.G., DUNFIELD P.F., McDONALD I.R., DEDYSH S.N., MIGUEZ C.B., MURRELL J.C. Regulation of methane oxidation in the facultative methanotroph Methylocella silvestris BL2. Mol. Microbiol. 58, 662, 2005.
• 7. MURRELL J.C., McDONALD I.R., GILBERT B. Regulation of expression of methane monooxygenases by copper ions. Trends Microbiol. 8, 221, 2000.
• 8. DUPERRON S., SLBUET M., McGREGOR B.J., KUYPERS M.M.M., FLSHER C.R., DUBLLLER N. Diversity, relative abundance and metabolic potential of bacterial endosymbionts Bathymodiolus mussel species from cold seeps in the Gulf of Mexico. Environ. Microbiol. 9, 1423, 2009.
• 9. DEDYSH S.N., DERAKSHANI M., LIESACK W. Detection and enumeration of methanotrophs in acidic Sphagnum peat by 16S rRNA fluorescence in situ hybridization, including the use of newly developed oligonucleotide probes for Methylocella palustris. Appl. Environ. Microbiol. 67, 4850, 2001.
• 10. MARTIN M., GIBELLO A., LOBO C., NANDE M., GARBI C., FAJARDO C., BARRA-CARACCIOLO A., GRENNI P., MARTINEZ-INIGO M.J. Application of fluorescence in situ hybridization technique to detect simazinedegrading bacteria in soil samples. Chemosphere 71, 703, 2008.
• 11. AMANN R., FUCHS B.M., BEHRENS S. The identification of microorganisms by fluorescence in situ hybridization. Curr. Op. Biotech. 12, 231, 2001.
• 12. CROCETTI G., MURTO M., BJÖRNSSON L. An update and optimization of oligonucleotide probes targeting methanogenic Archea for use in fluorescence in situ hybridization (FISH). J. Microbiol. Meth. 65, 194, 2006.
• 13. WHITTENBURY R., PHILIPS K.C., WILKINSON J.P. Enrichment, isolation and some properties of methane-utilizing bacteria. J. Gen. Microbiol. 61, 205, 1970.
• 14. SAMBROOK J., RUSSEL D.W. Molecular Cloning: a Laboratory Manual, 3rd ed.; Cold Spring Harbor Laboratory Press: New York, pp. 5-61, 1989.
• 15. KOTELNIKOVA S. Microbial production and oxidation of methane in deep subsurface. Earth Sci. Rev. 58, 367, 2001.
• 16. STĘPNIEWSKA Z., SZMAGARA A., STEFANIAK E. Optimization of environmental parameters conductive to methane oxidation with coal mine dump rock. Environ. Prot Eng. 30, 133, 2004.
• 17. STĘPNIEWSKA Z., PYTLAK A. Methanotrophic activity of coalbed rocks from “Bogdanka” coal mine (south-east Poland). Arch. Environ. Prot. 34, 183, 2008.
• 18. STĘPNIEWSKA Z., PYTLAK A. Distribution of methanotrophic activity of carbonaceous rocks from different seams of “Bogdanka” coal mine. Pol. J. Environ. Stud. 17, 550, 2008.
• 19. BECKMANN S., LUEDERS T., KRÜGER M., VON NETZER F., ENGELEN B., CYPIONKA H. Acetogens and acetoclastic Methanosarcinales govern methane formation in abandoned coal mines. Appl. Environ. Microbiol. doi:10.1128/AEM.02818-10, 2011.
• 20. BOURNE D.G., HOLMES A.J., IVERSEN N., MURRELL J.C. Fluorescent oligonucleotide rDNA probes for specific detection of methane oxidizing bacteria. FEMS Microbiol. Ecol. 31, 29, 2000.
• 21. LIPSKI A., FRIEDRICH U., ALTENDORF K. Application of rRNA-targeted oligonucleotide probes in biotechnology. Appl. Microbiol. Biotechnol. 56, 40, 2001.
• 22. ELLER G., FRENZEL P. Changes in activity and community structure of methane-oxidizing bacteria over the growth period of rice. Appl. Environ. Microbiol. 67, 2395, 2001.
• 23. SLOBODOVA N.V., KOLGANOVA T.V., BOULYGINA E.S., KUZNETSOV B.B., TOUROVA T.P., KRAVCHENKO I.K. Comparative characterization of methanotrophic enrichments by serological and molecular methods. Microbiol. 75, 336, 2006.
• 24. STĘPNIEWSKA Z., STEFAINAK E., SZMAGARA A. Methanotrophic activity of coal mine rocks. Soil-Plant Atmosphere Aeration and Environmental Problems, Institute of Agrophysics PAS: Lublin, pp. 103-108, 2004.