Analysis of IL-2 production in hookworm infected hamsters using generated polyclonal antibodies

• Autorzy: Dlugosz E. , Cendrowski J. , Baska P. , Siwinska A. , Wedrychowicz H. , Wisniewski M.
• Języki publikacji: EN

Abstrakty

EN

The aim of the study was cloning and anahsis of the entire coding sequence of hamster IL-2 by the method of RACE-PCR, its expression in Escherichia coli cells, and production of IL-2 specific antibodies. These antibodies were used to determine in vitro IL-2 production by cells derived from the spleen and mesenteric lymph nodes of Ancylostoma ceylanicum infected hamsters. The highest concentration of IL-2 was noted in supernatants from cell cultures coming from the oldest, most resistant hamsters.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2012
• Tom: 56
• Numer: 1
• Opis fizyczny: p.37-42,fig.,ref.

Twórcy

autor

Dlugosz E.

• 1Division of Parasitology and Parasitic Diseases, Warsaw University of Lifesciences - SGGW, 02-787 Warsaw, Poland

autor

Cendrowski J.

autor

Baska P.

autor

Siwinska A.

autor

Wedrychowicz H.

autor

Wisniewski M.

Bibliografia

• 1. Advant S.J., Braswell E.H., Kumar C.V., Kalonia D.S.: The effect of pH and temperature on the self-association of recombinant human interleukin-2 as studied by equilibrium sedimentation. Pharm Res 1995, 12, 637- 641.
• 2. Alkazmi L.M., Behnke J.M.: The mucosal response of hamsters to a low-intensity superimposed secondary infection with the hookworm Ancylostoma ceylanicum. J Helminthol 2011, 85, 56-65.
• 3. Bachmann M.F., Oxenius A.: Interleukin 2: from immunostimulation to immunoregulation and back again. EMBO Reports 2007, 8, 1142-1148.
• 4. Baggio V., Ott F., Fischer R.W., Gram H., Peele J., Spreng D., Schmökel H., Jungi T.W.: Production of antibodies to canine IL-1 beta and canine TNF to assess the role of proinflammatory cytokines. Vet Immunol Immunopathol 2005, 107, 27-39.
• 5. Bazan J.F.: Unraveling the structure of IL-2. Science 1992, 257, 410-413.
• 6. Bendtsen J.D., Nielsen H., von Heijne G., Brunak S.: Improved prediction of signal peptides: SignalP 3.0. J Mol Biol 2004, 340, 783-795.
• 7. Bungiro R.D., Sun T., Harrison L.M., Shoemaker C.B., Cappello M.: Mucosal antibody responses in experimental hookworm infection. Parasite Immunol 2008, 30, 293-303.
• 8. Cerretti D.P., McKereghan K., Larsen A., Cantrell M.A., Anderson D., Gills S., Cosman D., Baker P.E.: Cloning, sequence, and expression of bovine interleukin 2. Proc Natl Sci USA 1986, 83, 3223-3227.
• 9. Cuellar C., Wu W., Mendez S.: The hookworm tissue inhibitor of metalloproteases (Ac-TMP-1) modifies dendritic cell function and induces generation of CD4 and CD8 suppressor T cells. PLoS Negl Trop Dis 2009, 3, e439.
• 10. D'Cruz L.M., Klein L.: Development and function of agonist-induced CD25+Foxp3+ regulaton. T cells in the absence of interleukin 2 signaling. Nat Immun 2005, 6, 1152-1159.
• 11. Devos R., Plaetinck G., Cheroutre H., Simons G., Degrave W., Tavernier J., Remaut E., Fiers W.: Molecular cloning of human interleukin 2 cDNA and its expression in E. coli. Nucleic Acids Res 1983, 11, 4307- 4323.
• 12. Długosz E., Wiśniewski M., Wójcik L., Wędnchowicz H.: In -vitro IL-4, IL-12, and IFN-γ production by splenocytes from Ancylostoma ceylanicum infected hamsters. Bull Inst Vet Pulawy 2010, 54, 321-325.
• 13. Ferre F., Clote P.: DiANNA: a web server for disulfide connectivity prediction. Nucleic Acids Res 2005, 33, W230-W232.
• 14. Fontenot J.D., Rasmussen J.P., Gavin M.A., Rudensky A.Y.: A function for interleukin 2 in Foxp3-expressing regulatory T cells. Nat Immun 2005, 6, 1142-1151.
• 15. Fujita T.: Molecular cloning of human and murine interleukin-2 genes and their expression in various host cells. J Invest Dermatol 1985, 85, 180s-182s.
• 16. Geiger S.M., Caldas I.R., Mc Glone B.E., Campi- Azevedo A.C., De Oliveira M., Brooker S., Diemert D., Correa-Oliveira R., Bethony J.M.: Stage-specific immune responses in human Necator americanus infection. Parasite Immunol 2007, 29, 347-358.
• 17. Geiger S.M., Massara C.L., Bethony J., Soboslay P.T., Correa-Oliveira R.: Cellular responses and cytokine production in post-treatment hookworm patients from an endemic area in Brazil. Clin Exp Immunol 2004, 136, 334-340.
• 18. Ghosh K., Wu W., Antoine A.D., Bottazzi M.E., Valenzuela J.G., Hotez P.J., Mendez S.: The impact of concurrent and treated Ancylostoma ceylanicum hookworm infections on the immunogenicity of a recombinant hookworm vaccine in hamsters. J Infect Dis 2006, 193, 155-162.
• 19. Gillis S., Baker P.E., Ruscetti F.W., Smith K.A.: Long-term culture of human antigen-specific cytotoxic T-cell lines. J Exp Med 1978, 148, 1093-1098.
• 20. Haake D.A.: Hamster model of leptospirosis. Curr Protoc Microbiol 2006; Chapter 12 (Unit 12E 2).
• 21. Hanly W.C., Artwohl J.E., Bennett B.T.: Review of polyclonal antibody production procedures in mammals and poultry. ILAR J 1995, 37, 93-118.
• 22. Julenius K., Molgaard A., Gupta R., Brunak S.: Prediction, conservation analysis and structural characterization of mammalian mucin-type O-glycosylation sites. Glycobiology, 2005, 15, 153-164.
• 23. Klebb G., Autenrieth I.B., Haber H., Gillert E., Sadlack B., Smith K.A., Horak I.: Interleukin-2 is indispensable for development of immunological self-tolerance. Clin Immunol Immunopathol 1996, 81, 282-286.
• 24. Kovárová H., Stulík J., Macela A.: Interleukin 2. Biochemical aspects of structure and function. Sb Ved Pr Lek Fak Karlovy Univerzity Hradci Kralove Suppl 1989, 32, 397-411.
• 25. Lenardo M.J.: Interleukin-2 programs mouse αß T lymphocytes for apoptosis. Nature 1991, 353, 858-861.
• 26. Li J., Weghorst C.M., Tsutsumi M., Poi M.J., Knobloch T.J., Casto B.C., Melvin W.S., Tsai M., Muscarella P.: Frequent p16INK4A/CDNK2A alterations in chemically induced Syrian golden hamster pancreatic tumors. Carcinogenesis 2004, 25, 263-268.
• 27. Maruyama K., Takigawa Y., Akiyama Y., Hojo T., Nara- Ashizawa N., Cheng J., Watanabe M., Yamaguchi K.: Structure and characterization of hamster IL-12 p35 and p40. Mol Immunol 2003, 40, 319-326.
• 28. Melby P.C., Tryon V.V., Chandrasekar B., Freeman G.L.: Clonong of Syrian hamster (Mesocricetus auratus) cytokine cDNAs and analysis of cytokine mRNA expression in experimantal visceral leishmaniasis. Infect Immun 1998, 66, 2135-2142.
• 29. Mendez S., Valenzuela J.G., Wu W., Hotez P.J.: Host cytokine production, lymphoproliferation, and antibody responses during the course of Ancylostoma ceylanicum infection in the golden Syrian hamster. Infect Immun 2005, 73, 3402-3407.
• 30. Morar D., Tijhaar E., Negrea A., Hendriks J., van Haarlem D., Godfroid J., Michel A.L., Rutten V.P.: Cloning, sequencing and expression of white rhinoceros (Ceratotherium simum) interferon-gamma (IFN-γ) and the production of rhinoceros IFN-γ specific antibodies. Vet Immunol Immunopathol 2007, 115, 146-154.
• 31. Morgan D.A., Ruscetti F.W., Gallo R.C.: Selective in vitro growth of T lymphocytes from normal human bone marrow. Science 1976, 193, 1007-1009.
• 32. Qi J., Chen J., Wang J., Fang J., Wu J., Zhou J.: Expression of goose interleukin-2 gene in Escherichia coli and isolation of its soluble monomer. Chin J Biotechnol 2008, 24, 183-187.
• 33. Raimondi A., Cabrini R., Itoiz M.E.: Ploidy analysis of field cancerization and cancer development in the cheek pouch carcinogenesis model. J Oral Pathol Med 2005, 34, 227-231.
• 34. Refaeli Y., Van Parijs L., London C.A., Tschopp J., Abbas A.K.: Biochemical mechanisms of IL-2-regulated Fas-mediated T cell apoptosis. Immunity 1998, 8, 615-623.
• 35. Taniguchi T., Matsui H., Fujita T., Takaoka C., Kashima N., Yoshimoto R., Hamuro J.: Structure and expression of a cloned cDNA for human interleukin-2. Nature 1983, 302, 305-310.
• 36. Xiao S.Y., Guzman H., Zhang H., Travassos da Rosa A.P., Tesh R.B.: West Nile virus infection in the golden hamster (Mesocricelus auratus): a model for West Nile encephalitis. Emerg Infect Dis 2001, 7, 714-721.
• 37. Zhou J.Y., Wang J.Y., Chen J.G., Wu J.X., Gong H., Teng Q.Y., Guo J.Q., Shen H.G.: Cloning, in vitro expression and bioactivity of duck interleukin-2. Mol Immunol 2005, 45, 589-598.
• 38. Zurawski S.M., Vega Jr F., Doyle E.L., Huyghe B., Flaherty K., McKay D.B., Zurawski G.: Definition and spatial location of mouse interleukin-2 residues that interact with its heterotrimeric receptor. EMBO J 1993, 12, 5113-5119.