Fibronectin-the most potent factor in neural stem cells of HUCB-NSC line development is associated with MMPs activity

• Autorzy: Dragun-Szymczuk P. , Sypecka J. , Zalewska T.
• Języki publikacji: EN

Abstrakty

EN

Matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) are considered the main regulators of the cell microenvironment which governs NSC development. The aim of our study was to investigate whether the selected ECM components (laminin, fi bronectin, collagen) infl uence the neural stem cells proliferation and differentiation. In addition we checked if MMPs are engaged in the above developmental processes. Methods: The cells of HUCB-NSC line were seeded on the ECM components-coated plates. The serum-free medium was applied 48 h before experiments. On the 4th, 8th and 14th day in culture, cell proliferation assay and in situ zymography were performed, followed by the immunocytochemistry with the specifi c neural markers. Results: Our results showed that fi bronectin stimulated cell proliferation as well as MMPs activity most intensively (~20% increase in the 2-week cultured cells). It was also the most potent factor in promoting the cell differentiation, mostly toward neurons. We also checked the involvement of the MMPs in cell development. For this purpose we used MMPs inhibitors: GM6001 and TIMPs as well as inhibitors for serine and furin proteases ñ Pefabloc and DecRVKR-CMK. We found that only inhibitors of MMPs infl uence stem cells developmental processes. GM6001 down-regulated cell proliferation (~30%) and differentiation into neurons (~20%). The decrease of cell proliferation was also observed in the presence of TIMP2. In contrast, TIMP1 accelerated cell divisions. Conclusion: Our results demonstrate that fi bronectin is the potent factor in promoting the cell proliferation, differentiation, and support the participation of MMPs in the mechanism(s) responsible for neural stem cells maturation. Supported by MSRHE grants: 1266/P01/2006/31 and N40101832 /0296

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 1
• Opis fizyczny: p.106

Twórcy

autor

Dragun-Szymczuk P.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw Poland

autor

Sypecka J.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw Poland

autor

Zalewska T.

• NeuroRepair Department, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw Poland