Accumulation and translocation of Cd metal and the Cd-induced production of glutathione and phytochelatins in Vicia faba L.

• Autorzy: Cabala R. , Slovakova L. , El Zohri M. , Frank H.
• Języki publikacji: EN

Abstrakty

EN

Translocation of cadmium (Cd) in the tissues of Vicia faba, the water content in biomass, the biomass production, and the glutathione and phytochelatin tissue concentrations were studied and correlated with the plant sensitivity and/or tolerance to Cd. The total concentrations of Cd were determined by inductively coupled plasma/ mass spectrometry (ICP-MS), the concentrations of glutathione (GSH) and phytochelatins 2 and 3 (PC2 and PC3) were determined by on-line high performance liquid chromatography/electrospray-ionization tandem mass spectrometry (HPLC–ESI–MS–MS) in the roots and leaves of the sensitive and the tolerant cultivars of V. faba grown in Cd containing nutrient solutions (NS, 0–100 µmol l⁻¹ Cd²⁺). Both the cultivars of V. faba accumulate a major portion of Cd in the roots and only a minor part of ca. 4% in the leaves. The differences between the cultivars concerning Cd accumulation in leaves were apparent from higher Cd concentrations in NS and the Cd amount in the sensitive cultivar was approximately twice as high. In the roots, the differences between the cultivars in the Cd accumulation were only statistically significant with the highest Cd concentrations in NS, with the tolerant cultivar accumulating about 16% more of Cd compared to the sensitive one. The biomass production of the sensitive cultivar decreased approximately twice as fast with increasing Cd concentration in NS. The biomass water content decreased with increasing Cd concentration in NS in both the cultivars. In general, the GSH concentration did not linearly correlate with Cd accumulation, except for the roots of the sensitive cultivar where it was independent, and was higher in the sensitive cultivar than in the tolerant one in both the leaves and roots. The GSH concentration in leaves was approximately one order of magnitude higher than that in the roots for both the cultivars. The relationships between the PC and Cd concentrations in tissues were found nonlinear. At lower Cd accumulation levels, the PC concentrations followed an increase in the Cd accumulation in both the roots and leaves, whereas at higher Cd accumulations the relations differed between roots and leaves. In the roots, the PC concentrations decreased with increasing Cd accumulation, whereas the PC concentration in the leaves followed the decrease in the Cd accumulation.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 4
• Opis fizyczny: p.1239-1248,fig.,ref.

Twórcy

autor

Cabala R.

• Department of Analytical Chemistry, Faculty of Science, Charles University in Prague, Albertov 6, 128 43, Prague, Czech Republic

autor

Slovakova L.

• Department of Plant Physiology, Faculty of Natural Sciences, Comenius University in Bratislava, Bratislava, Slovak Republic

autor

El Zohri M.

• Botany Department, Faculty of Science, Assuit University, Assuit, Egypt

autor

Frank H.

• Environmental Chemistry and Ecotoxicology, University of Bayreuth, Bayreuth, Germany

Bibliografia

• Adriano DC (1986) Trace elements in the terrestrial environment. Springer, New York
• Beck A, Lendzian K, Oven M, Christmann A, Grill E (2003) Phytochelatin synthase catalyzes key step in turnover of glutathione conjugates. Phytochemistry 62:423–431
• Béraud E, Cotelle S, Leroy P, Férard JF (2007) Genotoxic effects and induction of phytochelatins in the presence of Cd in Vicia faba roots. Mutat Res 633:112–116
• Cabrera C, Ortega E, Lorenzo ML, Lopez MC (1998) Cd contamination of vegetable crops, farmlands, and irrigation waters. Rev Environ Contam Toxicol 154:55–81
• Cataldo DA, Garland TR, Wildung RE (1981) Cd uptake kinetics in intact soybean plants. Plant Physiol 68:835–839
• Cieslinski G, Nielsen GH, Hogue EJ (1996) Low-molecular-weight organic acids in rhizosphere soils of durum wheat and their effect on Cd bioaccumulation. Plant Soil 180:267–276
• Cobbett CS (2000) Phytochelatins and their roles in heavy metal detoxification. Plant Physiol 123:825–832
• Dixit V, Pandey V, Shyam R (2001) Differential antioxidative responses to Cd in roots and leaves of pea (Pisum sativum L. cv. Azad). J Exp Bot 52:1101–1109
• Ederli L, Reale L, Ferranti F, Pasqualini S (2004) Responses induced by high concentration of Cd in Phragmites australis roots. Physiol Plant 121:66–74
• El Zohri M, Čabala R, Frank H (2005) Quantification of phytochelatins in plants by reversed-phase HPLC–ESI–MS–MS. Anal Bioanal Chem 382:1871–1876
• Gorinova N, Nedkovska M, Todorovska E, Simova-Stoilova L, Stoyanova Z, Georgieva K, Demirevska-Kepova K, Atanasov A, Herzig R (2007) Improved phytoaccumulation of Cd by genetically modified tobacco plants (Nicotiana tabacum L.). Physiological and biochemical response of the transformants to Cd toxicity. Environ Pollut 145:161–170
• Greger M, Brammer E, Lindberg S, Larsson G, Idestam-Almquist J (1991) Uptake and physiological effects of Cd in sugar beet (Beta vulgaris) related to mineral provision. J Exp Bot 42:729–737
• Haag-Kerwer A, Schäfe HJ, Heiss S, Walter C, Rausch T (1999) Cd exposure in Brassica juncea caused a decline in transpiration rate and leaf expansion without effect on photosynthesis. J Exp Bot 50:1827–1835
• Hart JJ, Welch RM, Norvell WA, Sullivan LA, Kochian LV (1998) Characterization of Cd binding, uptake, and translocation in intact seedlings of bread and durum wheat cultivars. Plant Physiol 116:1413–1420
• Hernández LE, Cooke DT (1997) Modification of the root plasma membrane lipid composition of Cd treated Pisum sativum. J Exp Bot 48:1375–1381
• Howden R, Goldsborough PB, Andersen CD, Cobett CS (1995) Cdsensitive, cad1 mutants of Arabidopsis thaliana are phytochelatin deficient. Plant Physiol 107:1059–1066
• Jones JRE (1939) The relation between the electrolytic solution pressures of the metals and their toxicity to the stickleback (Gasterosteus aculeatus L.). J Exp Biol 16:425–437
• Kabata-Pendias A, Pendias H (1992) Trace elements in soils and plants, 2nd edn. CRC Press, Boca Raton
• Knecht JA, van Dillen M, Koevoets PLM, Schat H, Verkeij JAC, Ernst WHO (1994) Phytochelatins in Cd-sensitive and Cdtolerant Silene vulgaris. Plant Physiol 104:255–261
• Landberg T, Greger M (2004) No phytochelatin (PC2 and PC3) detected in Salix viminalis. Physiol Plant 121:481–487
• Li YM, Chaney RL, Schneiter AA, Miller JF (1995) Screening for low grain Cd phenotypes in sunflower, durum wheat and flax. Crop Sci 35:137–141
• Liu WJ, Zhu YG, Smith FA, Smith SE (2004) Do iron plaque and genotypes affect arsenate uptake and translocation by rice seedlings (Oryza sativa L.) grown in solution culture? J Exp Bot 55:1707–1713
• Liu CP, Shen ZG, Li XD (2007) Accumulation and detoxification of Cd in Brassica pekinensis and B. chinensis. Biol Plant 51:116–120
• Loscos J, Naya L, Ramos J, Clemente MR, Matamoros MA, Becana M (2006) A reassessment of substrate specificity and activation of phytochelatin synthases from model plants by physiologically relevant metals. Plant Physiol 140:1213–1221
• Lozano-Rodriguez E, Hernandez LE, Bonay P, Carpena-Riuz RO (1997) Distribution of Cd in shoot and root tissues of maize and pea plants: physiological disturbances. J Exp Bot 48:123–128
• Maier EA, Matthews RD, McDowell JA, Walden RR, Ahner BA (2003) Environmental Cd levels increase phytochelatin and glutathione in lettuce grown in a chelator-buffered nutrient solution. J Environ Qual 32:1356–1364
• Martinka M, Lux A (2006) Intraspecific variation of Silene dioica L. in uptake and translocation of Cd related to endodermal development. In: Teixeira da Silva JA (ed) Floriculture, ornamental and plant biotechnology. Advances and topical issues, vol III, 1st ed. edn. GSB, UK, pp 312–316
• Mathys W (1973) Vergleichende Untersuchungen der Zinkaufnahme von resistent und sensitiven Populationen von Agrostis tenuis Sibth. Flora Jena 162:492–499
• Mathys W (1975) Enzymes of heavy metal-resistant and nonresistant populations of Silene cucubalus and their interactions with some heavy metals in vitro and in vivo. Physiol Plant 33:161–165
• Meister A (1995) Glutathione biosynthesis and its inhibition. Method Enzymol 251:3–7
• Noctor G, Strohm M, Jouanin L, Kunert KJ, Foyer CH, Rennenberg H (1996) Synthesis of glutathione in leaves of transgenic poplar overexpressing γ-glutamylcysteine synthetase. Plant Physiol 11:1071–1078
• Noctor G, Arisi ACM, Jouanin L, Kunert KJ, Rennenberg H, Foyer CH (1998) Glutathione: biosynthesis, metabolism and relationship to stress tolerance explored in transformed plants. J Exp Bot 49:623–647
• Oven M, Raith K, Neubert RHH, Kutchan TM, Zenk MH (2001) Homo-phytochelatins are synthesized in response to Cd in azuky beans. Plant Physiol 126:1275–1280
• Penner GA, Clark J, Bezte LZ, Lisle D (1995) Identification of RAPD markers linked to a gene governing Cd uptake in durum wheat. Genome 38:543–547
• Persson DP, Hansen TH, Holm PE, Schjoerring JK, Hansen HCB, Nielsen J, Cakmak I, Husted S (2006) Multi-elemental speciation analysis of barley genotypes differing in tolerance to Cd toxicity using SEC-ICP-MS and ESI-TOF-MS. J Anal Atom Spectrom 21:996–1005
• Pomponi M, Censi V, Di Girolamo V, De Paolis A, Sanita di Toppi L, Aromolo R, Costantino P, Cardarelli M (2006) Overexpression of Arabidopsis phytochelatin synthase in tobaco plants enhances Cd²⁺ tolerance and accumulation but not translocation to the shoot. Planta 223:180–190
• Poschenrieder C, Gunsé B, Barceló J (1989) Influence of Cd on water relations, stomatal resistance, and abscisic acid content in expanding bean leaves. J Plant Physiol 90:1365–1371
• Ramos J, Clemente MR, Naya L, Loscos J, Pérez-Rentomé C, Sato S, Tabata S, Becana M (2007) Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to Cd and alternatively spliced variants. Plant Physiol 143:1110–1118
• Ric De Voss CHR, Vonk MJ, Vooijs R, Schat H (1992) Glutathione depletion due to copper-induced phytochelatin synthesis causes oxidative stress in Silene cucubalus. Plant Physiol 98:853–858
• Rodecap KD, Tingey DT, Tibbs JH (1981) Cd-induced ethylene production in bean plant. Z Pflanzenphysiol 105:65–74
• Ruegsegger A, Brunold C (1992) Effect of Cd on ‘y-glutamylcysteine synthesis in maize seedlings. Plant Physiol 99:428–433
• Sanita di Toppi L, Gabbrielli R (1999) Response to Cd in higher plants. Environ Exp Bot 41:105–130
• Slováková L, Klecová-Šimonová E, Henselová M, Hudák J (2007) Antioxidant enzymes of tolerant (Brassica juncea L.) and susceptible [Vigna radiata (L.) Wilczek] plants to Cd. In: Bláha L (ed) Influence of abiotic and biotic stressors to property of plants 2007. Proceedings VÚRV v.v.i., Prague-Ruzyne, pp 355–362
• Sriprang R, Hayashi M, Ono H, Takagi M, Hirata K, Murooka Y (2003) Enhanced accumulation of Cd²⁺ by a Mesorhizobium sp. transformed with a gene from Arabidopsis thaliana coding for phytochelatin synthase. Appl Environ Microbiol 69:1791–1796
• Srivastava S, Tripathi RD, Dwivedi UN (2004) Synthesis of phytochelatins and modulation of antioxidants in response to Cd stress in Cuscuta reflexa—an angiospermic parasite. J Plant Physiol 161:665–674
• Steffens JC (1990) The heavy metal-binding peptides of plants. Annu Rev Plant Mol Biol 41:553–575
• Sun Q, Wang XR, Ding SM, Yuan XF (2005) Effects of interaction between Cd and plumbum on phytochelatins and glutathione production in wheat (Triticum aestivum L.). J Integr Plant Biol (Acta Bot Sin) 47:435–442
• Szalai G, Janda T, Galan-Goldhirsh A, Páldi E (2002) Effect of Cd treatment on phytochelatin synthesis in maize. Acta Biol Szeged 46:121–122
• Tsyganov VE, Belimov AA, Borisov AY, Safronova VI, Georgi M, Dietz KJ, Tikhonovich IA (2007) A chemically induced new pea (Pisum sativum) mutant SGECdt with increased tolerance to, and accumulation of, Cd. Ann Bot 99:227–237
• Van Bruwaene R, Kirchmann R, Impens R (1984) Cd contamination in agriculture and zootechnology. Experientia 40:43–52
• Vatamaniuk OK, Mari S, Lu YP, Rea PA (2000) Mechanism of heavy metal ion activation of phytochelatin (PC) synthase. J Biol Chem 275:31451–31459
• Vázqez S, Goldsbrough P, Carpena RO (2009) Comparative analysis of the contribution of phytochelatins to Cd and arsenic tolerance in soybean and white lupin. Plant Physiol Biochem 47:63–67
• Villalobos-Pietrini R, Flores-Marquez AR, Gomez-Arroyo S (1994) Cytogenetic effects in Vicia faba of the polluted water from rivers of the Tlaxcala hydrological system, Mexico. Rev Int Contam Ambient 10:83–88
• Yen TY, Villa JA, DeWitt JG (1999) Analysis of phytochelatin–Cd complexes from plant tissue culture using nano-electrospray ionization tandem mass spectrometry and capillary liquid chromatography/electrospray ionization tandem mass spectrometry. J Mass Spectrom 34:930–941
• Zhao FJ, Hamon RE, Lombi E, McLaughlin MJ, McGrath SP (2002) Characteristics of Cd uptake in two contrasting ecotypes of the hyperaccumulator Thlaspi caerulescens. J Exp Bot 53:535–543

Uwagi

PL

Rekord w opracowaniu