Bioactive domains controlling neural stem cells fate decisions

• Autorzy: Zychowicz M. , Buzanska L.
• Języki publikacji: EN

Abstrakty

EN

Studying in vitro mutual interactions of the stem cells with the components of their microenvironment mimicking in vivo conditions is crucial for the further tissue engineering and regenerative medicine applications. Bioactive domains obtained by nano/micro emerging technologies, such as microcontact printing or piezoelectric spotting, were designed to reflect the stem cell niche composition and to influence their fate decisions. Different geometry of biofunctionalized surfaces, obtained by microcontact printing of poly-L-lysine or fibronectin, was created to study the adhesion, migration, proliferation as well as the differentiation of human cord blood-derived neural stem cells (HUCB-NSCs). The bioactive domains microspotted on the cell repellant surface contained extracellular matrix protein - fibronectin enriched with small signaling molecules, (CNTF, Jagged, Wnt, Shh, Dkk) were designed to activate particular molecular pathways leading to the maintenance of the self renewal of non-differentiated cells or to promote their differentiation into different neural lineages. Our results revealed how to control neural stem cell fate decisions by manipulating with the architecture and composition of the niche bioactive domains in vitro. Moreover, such domains can be used to investigate the stem cell response to cyto- or neurotoxins (MeHgCl), thus establishing good tool for screening the effect of diverse factors influencing neural stem cell development. Sponsored by Polish Ministry of Scientific Research and Higher Education grant No: 2211/B/P01/2010/38 and European Commission Joint Research Centre NanoBioscience Action

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: S
• Opis fizyczny: p.19

Twórcy

autor

Zychowicz M.

• NeuroRepair Department, Mossakowski Medical Research Centre PAS, Warsaw, Poland

autor

Buzanska L.

• NeuroRepair Department, Mossakowski Medical Research Centre PAS, Warsaw, Poland

Uwagi

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