Isolation of cold stress-responsive genes from Lepidium latifolium by suppressive subtraction hybridization

• Autorzy: Aslam M. , Sinha V. B. , Singh R. K. , Anandhan S. , Pande V. , Ahmed Z.
• Języki publikacji: EN

Abstrakty

EN

Suppression subtraction hybridization (SSH) libraries were constructed from RNA isolated from leaves of control and cold stress-induced Lepidium latifolium, a cold-tolerant plant species from high altitudes for isolation of cold-responsive genes. A total of 500 clones were obtained from the cold stress library. Dot blot expression analysis identified 157 clones that were upregulated and 75 that were downregulated during cold stress. These clones selected on the basis of their expression patterns on dot blot were sequenced. As much as 27 and 17 genes were identified from the forward and reverse libraries, respectively. The genes identified revealed homology with genes involved in diverse processes such as gene regulation/signaling, photosynthesis, DNA damage repair protein, pathogenesis-related protein, senescence-associated proteins and proteins with unknown functions.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2010
• Tom: 32
• Numer: 1
• Opis fizyczny: p.205-210,fig.,ref.

Twórcy

autor

Aslam M.

• Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences

autor

Sinha V. B.

• Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences

autor

Singh R. K.

• Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences

autor

Anandhan S.

• Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences

autor

Pande V.

• Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences

autor

Ahmed Z.

• Franciszek Górski Institute of Plant Physiology of the Polish Academy of Sciences

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