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2017 | 20 | 1 |

Tytuł artykułu

Germocyte lesions caused by porcine circovirus type 2b in Kunming mice

Treść / Zawartość

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Porcine circovirus type 2 (PCV2) has been known as a causative agent of reproductive failure in the sow. In the present study mouse model was used to investigate PCV2 infection. In order to investigate whether PCV2 can induce lesions of spermatocytes and oocytes, 6 male and 6 female mice were each inoculated intraperitoneally with PCV2b, and 3 male and 3 female mice mock-infected with cell culture supernatant served as controls. Samples of testes and ovaries from PCV2b-inoculated and mock-infected mice were investigated using PCR, histopathological, ultrastructural and immunofluorescent histochemical methods at 14 and 21 day post infection (dpi). The study revealed that in the virus-inoculated mice leydig cells in testes and granulosa cells in ovaries were degenerated, and a small number of spermatocytes and oocytes showed apoptosis. Positive PCV2b antigen signals were also observed in these apoptotic cells. It can be suggested that PCV2 can cause lesions of spermatocyte and oocyte prior to zygotes formation in its host.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

20

Numer

1

Opis fizyczny

p.25-29,fig.,ref.

Twórcy

autor
  • College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, China
autor
  • College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, China
autor
  • College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, China
autor
  • College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan 410128, China

Bibliografia

  • Bielański A, Larochelle R, Algire J, Magar R (2004) Distribution of PCV-2 DNA in the reproductive tract, oocytes and embryos of PCV-2 antibody-positive pigs. Vet Rec 155: 597-598.
  • de Castro AM, Cruz TF, Salgado VR, Kanashiro TM, Ferrari KL, Araujo JP Jr, Brandão PE, Richtzenhain LJ (2012) Detection of porcine circovirus genotypes 2a and 2b in aborted foetuses from infected swine herds in the State of S4o Paulo, Brazil. Acta Vet Scand 54: 29.
  • Deng ZB, Gong QL, Wang ND, Yuan AW, Yu XL (2013a) Vertical transmission of porcine circovirus 2b (PCV2b) to mouse fetuses in maternal uterus by artificial insemination with semen from PCV2b-infected male mouse. Turk J Vet Anim Sci 37: 475-478.
  • Deng ZB, Yuan AW, LuoW,Wang ND, Gong QL, Yu XL, Xue LQ (2013b) Transmission of porcine circovirus type 2b (PCV2b) in Kunming mice. Acta Vet Hung 61: 234-243.
  • Karuppannan AK, Ramesh A, Reddy YK, Ramesh S, Mahaprabhu R, Jaisree S, Roy P, Sridhar R, Pazhanivel N, Sakthivelan SM, Sreekumar C, Murugan M, Jaishankar S, Gopi H, Purushothaman V, Kumanan K, Babu M (2016) Emergence of Porcine Circovirus 2 Associated Reproductive Failure in Southern India. Transbound Emerg Dis 63: 314-320.
  • Madson DM, Opriessnig T (2011) Effect of porcine circovirus type 2 (PCV2) infection on reproduction: disease, vertical transmission, diagnostics and vaccination. Anim Health Res Rev 12: 47-65.
  • Madson DM, Ramamoorthy S, Kuster C, Pal N, Meng XJ, Halbur PG, Opriessnig T (2008) Characterization of shedding patterns of Porcine circovirus types 2a and 2b in experimentally inoculated mature boars. J Vet Diagn Invest 20: 725-734.
  • Sarli G, Morandi F, Panarese S, Bacci B, Ferrara D, Bianco C, Fusaro L, Bacci ML, Galeati G, Dottori M, Bonilauri P, Lelli D, Leotti G, Vila T, Joisel F, Allan G, Benazzi C, Ostanello F (2012) Reproduction in porcine circovirus type 2 (PCV2) seropositive gilts inseminated with PCV2b spiked semen. Acta Vet Scand 54: 51.
  • Tummaruk P, Pearodwong P (2016) Expression of PCV2 antigen in the ovarian tissues of gilts. J Vet Med Sci 78: 457-461.
  • Yoon KJ, Jepsen RJ, Pogranichniy RM, Sorden S, Stammer R, Evans LE (2004) A novel approach to intrauterine viral inoculation of swine using PCV type 2 as a model. Theriogenology 61: 1025-1037.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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