Identification and molecular characterisation of HMW glutenin subunit 1By16* in wild emmer

• Autorzy: Jin M. , Xie Z.-Z. , Ge P. , Li J. , Jiang S.-S. , Subburaj S. , Li X.-H. , Zeller F.-J. , Hsam S.-L. K. , Yan Y.-M.
• Języki publikacji: EN

Abstrakty

EN

In this study, a novel y-type high molecular weight glutenin subunit (HMW-GS) in wild emmer wheat Triticum turgidum L. var. dicoccoides (Körn.) accession KU1952 was identified by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), capillary electrophoresis (CE) and matrix-assisted laser desorption ionisation/time-of-flight/mass spectrometry (MALDI-TOF-MS). Its electrophoretic mobility and molecular weight were similar to those of 1By16 and was designated as 1By16*. The complete coding sequence of the 1By16* gene isolated by allelic-specific polymerase chain reaction (AS-PCR) consists of 2,157 bp, encoding 729 amino acid residues. The real presence and authenticity of the 1By16* gene in KU1952 were further confirmed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), heterologous expression and Western blotting. The molecular structure as well as phylogenetic analysis revealed that 1By16* had 21 single-nucleotide polymorphism (SNP) variations and possessed greater similarity with superior quality subunits 1By15 and 1By16 of common wheat. Secondary structure prediction displayed higher α-helix and β-strand contents in the 1By16* subunit, which could form a superior gluten structure and, consequently, might have positive effects on dough quality. Our results suggest that 1By16* is expected to be a new potential gene for wheat quality improvement.

• Wydawca: -
• Czasopismo: Journal of Applied Genetics
• Rocznik: 2012
• Tom: 53
• Numer: 3
• Opis fizyczny: p.249-258,fig.,ref.

Twórcy

autor

Jin M.

• Key Laboratory of Genetics and Biotechnology, College of Life Science, Capital Normal University, Beijing 100048, China

autor

Xie Z.-Z.

autor

Ge P.

autor

Li J.

autor

Jiang S.-S.

autor

Subburaj S.

autor

Li X.-H.

autor

Zeller F.-J.

autor

Hsam S.-L. K.

autor

Yan Y.-M.

Uwagi

PL

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