Electrofusion of protoplasts from Solanum tuberosum, S. bulbocastanum and S. pinnatisectum

• Autorzy: Greplova M. , Polzerova H. , Vlastnikova H.
• Języki publikacji: EN

Abstrakty

EN

This paper discusses a number of experiments performed, involving the fusion by an electric field of mesophyll protoplasts from Solanum tuberosum cv. Bintje, S. tuberosum dihaploid clones 243, 299 and the wild tuberous disease-resistant species S. bulbocastanum and S. pinnatisectum. Three fusion experiments (S. bulbocastanum + S. tuberosum dihaploid 243, S. pinnatisectum + S. tuberosum cv. Bintje and S. pinnatisectum+S. Tuberosum dihaploid 299) yielded 542 calli, the 52 ones of which produced shoots. Obtained regenerants were estimated by the flow-cytometry (FC) and RAPD analysis to determine hybrid plants.The utilisation of the FC as a useful method for detecting somatic hybrids is also discussed in this paper. The combination S. bulbocastanum + S. tuberosum dihaploid 243 led to the creation of eight somatic hybrids, the combination S. pinnatisectum + S. tuberosum cv. Bintje yielded four somatic hybrids and the combination S. pinnatisectum + S. tuberosum dihaploid 299 resulted in no hybrid regenerants. Morphology in vitro, growth vigour and production of tuber-like structures were evaluated in hybrid plants. Plants were transferred in vivo for further estimation (acclimatization, habitus evaluation and tuberization ability).

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 6
• Opis fizyczny: p.787-796,fig.,ref.

Twórcy

autor

Greplova M.

• Laboratory of Experimental Breeding, Potato Research Institute Havlickuv Brod, Ltd., 2366 Dobrovskeho, 580 01 Havlickuv Brod, Czech Republic

autor

Polzerova H.

• Laboratory of Experimental Breeding, Potato Research Institute Havlickuv Brod, Ltd., 2366 Dobrovskeho, 580 01 Havlickuv Brod, Czech Republic

autor

Vlastnikova H.

• Laboratory of Experimental Breeding, Potato Research Institute Havlickuv Brod, Ltd., 2366 Dobrovskeho, 580 01 Havlickuv Brod, Czech Republic

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