Modeling artifact in the living neural networks stimulating system

• Autorzy: Kolodziej K. , Hottowy P.
• Języki publikacji: EN

Abstrakty

EN

INTRODUCTION: Electrical stimulation of neurons results in large artifacts that makes recording of the stimulated activity difficult. In particular, detection of low‑latency spikes from directly activated neurons at the stimulating electrodes remains virtually impossible. AIM(S): We tested a new idea for artifact reduction, based on an optimized correction pulse (CP) applied to the stimulating electrode instantly after the stimulation pulse (SP). While being generated, the CP would induce the exact opposite of the artifact initiated by the SP. The signal distortion would be minimized, allowing for detection of the neuronal response during application of the CP. METHOD(S): Based on realistic model of the electrode impedance we estimated the shape of the stimulation artifact and calculated the optimal shape of the CP. We analyzed the hardware limitations of the stimulation circuit and its impact on the reduced artifact amplitude.We also considered the effects ofthe impedance model inaccuracy, as the real-life experiment will be based on impedance measurements with limited precision. We analyzed the artifact level at the output of the recording amplifierto take into accountits filtering properties. RESULTS: We analyzed the artifact reduction procedure for typical symmetric biphasic SP and impedance model for 5-micron platinium electrode. Even 2 microampere pulse without the CP generated the artifact that saturated the amplifier for at least 300 microseconds following the SP. Simulations confirmed that the optimal CP reduced the artifact almost completely. More importantly, even when electrode impedance was given with 5% error, the artifact was reduced more than 10 times for the first 300 microseconds after the SP, compared with the SP without correction. CONCLUSIONS: Numerical simulations suggest that our method will allow for reliable spikes recording even on the stimulating electrode. The experimental validation will take advantage of the novel stimulation/recording system currently being developed in our laboratory. FINANCIAL SUPPORT: This work was supported by Polish National Science Centre grant DEC-2013/10/M/ NZ4/00268.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2017
• Tom: 77
• Numer: Suppl.1
• Opis fizyczny: p.132

Twórcy

autor

Kolodziej K.

• Department of Particle Interactions and Detection Techniques, AGH University of Science and Technology, Cracow, Poland

autor

Hottowy P.

• Department of Particle Interactions and Detection Techniques, AGH University of Science and Technology, Cracow, Poland