Molecular cloning and characterization of a pathogenesis-related protein SmPR10-1 from Salvia miltiorrhiza

• Autorzy: Zhao L. , Ma L.-g. , Wang Z.-s , Chen M.-L. , Shen Y. , Huang L.-q.
• Języki publikacji: EN

Abstrakty

EN

A full-length cDNA, SmPR10-1, encoding a pathogenesis-related class 10 protein, was isolated from Salvia miltiorrhiza, a well-known Chinese herbal plant. From the coding region of nucleotide sequence, the amino acid sequence can be predicted which contained conserved domain (K–A–X–E–X–Y) in the C-terminal helix found in most members of the PR10 protein family. Phylogenetic tree analysis indicated that the SmPR10-1 protein showed a sequence similarity which was much higher to dicot proteins than to other PR10 proteins. Prokaryotic expression of SmPR10-1 protein in fusion with a His-tag produced a 22 kDa protein in E. coli BL21 (DE3), which exhibited ribonuclease activity in vitro. The purified protein was used to assay antifungal activity and the results showed SmPR10-1 only inhibited the growth of Phytophthora infestans. The RT-PCR results showed that the SmPR10-1 was expressed in high transcript level in leaves, stalks and low levels in roots. This protein seems to involve in the plant active defense response through activation of the methyl jasmonate signaling pathway rather than salicylic acid or abscisic acid pathway.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 10
• Opis fizyczny: Article: 205 [8 p.], fig.,ref.

Twórcy

autor

Zhao L.

• State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China
• School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, China

autor

Ma L.-g.

• State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China
• School of Pharmacy, Henan University of Traditional Chinese Medicine, Zhengzhou, 450046, China

autor

Wang Z.-s

• State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

autor

Chen M.-L.

• State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

autor

Shen Y.

• State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

autor

Huang L.-q.

• State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

Bibliografia

• Adie BAT, Perez-Perez J, Perez-Perez MM et al (2007) ABA is an essential signal for plant resistance to pathogens affecting JA biosynthesis and the activation of defenses in Arabidopsis. Plant Cell 19:1665–1681
• Arnold K, Bordoli L, Kopp J, Schwede T (2006) The SWISSMODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22:195–201
• Bari R, Jones J (2009) Role of plant hormones in plant defence responses. Plant Mol Biol 69:473–488
• Barratt DHP, Clark JA (1991) Proteins arising during the late stages of embryogenesis in Pisum sativum L. Planta 184:14–23
• Breiteneder H, Pettenburger K, Bito A et al (1989) The gene coding for the major birch pollen allergen Betv1, is highly homologous to a pea disease resistance response gene. EMBO J 8:1935–1938
• Bufe A, Spangfort MD, Kahlert H et al (1996) The major birch pollen allergen, Bet v 1, shows ribonuclease activity. Planta 199:413–415
• Chadha P, Das RH (2006) A pathogenesis related protein, AhPR10 from peanut: an insight of its mode of antifungal activity. Planta 225:213–222
• Cheng TO (2006) Danshen: a popular Chinese cardiac herbal drug. J Am Coll Cardiol 47:1499–1500
• Crowell DN, John ME, Russell D, Amasino RM (1992) Characterization of a stress-induced, developmentally regulated gene family from soybean. Plant Mol Biol 18:459–466
• Dangl JL, Jones JD (2001) Plant pathogens and integrated defence responses to infection. Nature 411:826–833
• Emanuelsson O, Brunak S, von Heijne G, Nielsen H (2007) Locating proteins in the cell using TargetP, SignalP and related tools. Nature Protocol 2:953–971
• Fernandes H, Michalska K, Sikorski M, Jaskolski M (2013) Structural and functional aspects of PR-10 proteins. FEBS J 280:1169–1199
• Flores T, Alape-Giron A, Flores-Diaz M, Flores HE (2002) Ocatin. A novel tuber storage protein from the andean tuber crop oca with antibacterial and antifungal activities. Plant Physiol 128:1291–1302
• Gajhede M, Osmark P, Poulsen FM et al (1996) X-ray and NMR structure of Bet v 1, the origin of birch pollen allergy. Nat Struct Biol 3:1040–1045
• Gao ZS, van de Weg WE, Schaart JG et al (2005) Genomic cloning and linkage mapping of the Mal d 1 (PR-10) gene family in apple (Malus domestica). Theor Appl Genet 111:171–183
• Gao W, Hillwig ML, Huang L et al (2009) A functional genomics approach to tanshinone biosynthesis provides stereochemical insights. Org Lett 11:5170–5173
• Ge XC, Wu JY (2005) Tanshinone production and isoprenoid pathways in Salvia miltiorrhiza hairy roots induced by Ag? and yeast elicitor. Plant Sci 168:487–491
• Gómez-Gómez L, Rubio-Moraga A, Ahrazem O (2011) Molecular cloning and characterisation of a pathogenesis-related protein CsPR10 from Crocus sativus. Plant Biol 13:297–303
• Guo J et al (2013) CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts. Proc Natl Acad Sci USA 110:12108–12113
• Hashimoto M, Kisseleva L, Sawa S et al (2004) A novel rice PR10 protein, RSOsPR10, specifically induced in roots by biotic and abiotic stresses, possibly via the jasmonic acid signaling pathway. Plant Cell Physiol 45:550–559
• Hoagland DR, Arnon DI (1950) The water-culture method for growing plants without soil. University of California, Berkeley, pp 30–32
• Hoffmann-Sommergruber K, Vanek-Krebitz M, Radauer C et al (1997) Genomic characterization of members of the Bet v 1 family: genes coding for allergens and pathogenesis-related proteins share intron positions. Gene 197:91–100
• Jwa NS, Kumar Agrawal G, Rakwal R et al (2001) Molecular cloning and characterization of a novel Jasmonate inducible pathogenesis-related class 10 protein gene, JIOsPR10, from rice (Oryza sativa L.) seedling leaves. Biochem Biophys Res Commun 286:973–983
• Kim ST, Yu S, Kang YH et al (2008) The rice pathogen-related protein 10 (JIOsPR10) is induced by abiotic and biotic stresses and exhibits ribonuclease activity. Plant Cell Rep 27:593–603
• Lee EJ, Facchini P (2010) Norcoclaurine synthase is a member of the pathogenesis-related 10/Bet v1 protein family. Plant Cell 22:3489–3503
• Liu JJ, Ekramoddoullah AK (2006) The family 10 of plant pathogenesis-related proteins: their structure, regulation, and function in response to biotic and abiotic stresses. Physiol Mol Plant Pathol 68:3–13
• Liu JJ, Ekramoddoullah AKM, Yu XS (2003) Differential expression of multiple PR10 proteins in western white pine following wounding, fungal infection and cold-hardening. Physiol Plant 119:544–553
• Liu X, Huang B, Lin J et al (2006) A novel pathogenesis-related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen-inducible. J Plant Physiol 163:546–556
• Ma Y, Yuan L, Wu B et al (2012) Genome-wide identification and characterization of novel genes involved in terpenoid biosynthesis in Salvia miltiorrhiza. J Exp Bot 63:2809–2823
• Markovic-Housley Z, Degano M, Lamba D et al (2003) Crystal structure of a hypoallergenic isoform of the major birch pollen allergen Bet v 1 and its likely biological function as a plant steroid carrier. J Mol Biol 325:123–133
• Matton DP, Brisson N (1989) Cloning, expression, and sequence conservation of pathogenesis-related gene transcripts of potato. Mol Plant-Microbe Interact 2:325–331
• Midoh N, Iwata M (1996) Cloning and characterization of a probenazole-inducible gene for an intracellular pathogenesisrelated protein in rice. Plant Cell Physiol 37:9–18
• Park CJ, Kim KJ, Shin R et al (2004) Pathogenesis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviral pathway. Plant J 37:186–198
• Rost B, Yachdav G, Liu J (2004) The PredictProtein server. Nucl Acid Res 32:W321–W326
• Schwede T, Kopp J, Guex N, Peitsch MC (2003) SWISS-MODEL: an automated protein homology-modeling server. Nucl Acid Res 31:3381–3385
• Sels J, Mathys J, De Coninck BM et al (2008) Plant pathogenesisrelated (PR) proteins: a focus on PR peptides. Plant Physiol Biochem 46:941–950
• Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
• van Loon LC, Rep M, Pieterse CM (2006) Significance of inducible defense-related proteins in infected plants. Annu Rev Phytopathol 44:135–162
• Walter MH, Liu JW, Wunn J, Hess D (1996) Bean ribonuclease-like pathogenesis-related protein genes (Ypr10) display complex patterns of developmental, dark-induced and exogenous-stimulus-dependent expression. Eur J Biochem 239:281–293
• Warner SAJ, Scott R, Draper J (1992) Characterisation of a woundinduced transcript from the monocot asparagus that shares similarity with a class of intracellular pathogenesis-related (PR) proteins. Plant Mol Biol 19:555–561
• Wenping H, Yuan Z, Jie S, Lijun Z, Zhezhi W (2011) De novo transcriptome sequencing in Salvia miltiorrhiza to identify genes involved in the biosynthesis of active ingredients. Genomics 98:272–279
• Wilson CM (1975) Plant nucleases. Annu Rev Plant Physiol 26:187–208
• Xie YR, Chen ZY, Brown RL, Bhatnagar D (2010) Expression and functional characterization of two pathogenesis-related protein 10 genes from Zea mays. J Plant Physiol 167:121–130
• Yan Q, Shi M, Ng J, Wu HY (2006) Elicitor-induced rosmarinic acid accumulation and secondary metabolism enzyme activities in Salvia miltiorrhiza hairy roots. Plant Sci 170:853–858
• Zhou XJ, Lu S, Xu YH et al (2002) A cotton cDNA (GaPR-10) encoding a pathogenesis-related 10 protein with in vitro ribonuclease activity. Plant Sci 162:629–636

Identyfikatory

DOI

10.1007/s11738-015-1959-8