Production of cellulases by Bacillus cellulosilyticus using lignocellulosic material

• Autorzy: Maryam B. , Qadir A. , Zameer M. , Ahmad S.R. , Nelofer R. , Jamil N. , Arzoo S. , Afzaal R.
• Języki publikacji: EN

Abstrakty

EN

The enzyme cellulase, a multi enzyme complex made up of several proteins, catalyses the conversion of cellulose to glucose in an enzymatic hydrolysis. In this study biomass alkali-pretreatment followed by enzymatic hydrolysis was carried out using crude cellulase enzyme produced from screened bacillus strain (Bacillus cellulosilyticus) having 1,998.79 IU/ml/min CMCase and 1,621.16 IU/ml/min FPase enzyme activity. The production of cellulase enzyme using an economical medium has been a significant achievement in the field of industrial biotechnology. The maximum yield of sugars in the form of total sugars (179.84±0.2 mg/ml), reducing sugars (126.72±0.1 mg/ml), and glucose (105.40±0.1 mg/ml) was achieved at 48 h incubation time, 50ºC, 5 pH, 6% enzyme concentration, 4% substrate loading, and PEG 3350 as a surfactant.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2018
• Tom: 27
• Numer: 6
• Opis fizyczny: p.2659-2667,fig.,ref.

Twórcy

autor

Maryam B.

• College of Earth and Environmental Sciences, (CEES) University of Punjab, Quaid-e-Azam campus, Lahore

autor

Qadir A.

• College of Earth and Environmental Sciences, (CEES) University of Punjab, Quaid-e-Azam campus, Lahore

autor

Zameer M.

• College of Earth and Environmental Sciences, (CEES) University of Punjab, Quaid-e-Azam campus, Lahore

autor

Ahmad S.R.

• College of Earth and Environmental Sciences, (CEES) University of Punjab, Quaid-e-Azam campus, Lahore

autor

Nelofer R.

• PCSIR Laboratories, Lahore

autor

Jamil N.

• College of Earth and Environmental Sciences, (CEES) University of Punjab, Quaid-e-Azam campus, Lahore

autor

Arzoo S.

• College of Earth and Environmental Sciences, (CEES) University of Punjab, Quaid-e-Azam campus, Lahore

autor

Afzaal R.

• College of Earth and Environmental Sciences, (CEES) University of Punjab, Quaid-e-Azam campus, Lahore

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Identyfikatory

DOI

10.15244/pjoes/80867