Diagnostic specificity of the mercaptoethanol test in the diagnosis of brucellosis in cattle. II. Effect of mercaptoaethanol on the agglutinating activity of sera of cattle vaccinated with Brucella Buck 19

• Autorzy: Anczykowski F.
• Języki publikacji: EN

Abstrakty

EN

Thirty days after vaccination with Brucella Buck 19 sera of two populations of heifers aging 6-8 months (in total 96), as well as sera or 618 cows aging 2-13 years, vaccinated 2-10 years ago, were examined. The mercaptoaethanol test (ME) titre of the heifers varied from the same titre like that of the conventional tube agglutination test (APS) to that of 8 times or more lower, and the ME titre of cows from the same titre to the 16 times lower (≥ 4 tube dilutions). Amplitudes of the ME test titres were different in particular herds of cows and proved to be dependent to a high degree also on the length of the post-vaccination period. Bath in heifers and adult cattle a close relationship occurred between the dispersion degree of the ME test titre levels and the frequency of particular levels or these titres - on the one hand, and the APS test titre level – on the other hand. This heterogeneity plotted in coordinate system a normal curve with the peak at the APS titre of 200-400 I.U. of heifer's sera and at 100 I.U. of cows' ones.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 1975
• Tom: 19
• Numer: 1-2
• Opis fizyczny: p.7-14,fig.,ref.

Twórcy

autor

Anczykowski F.

• Laboratory of Brucellosis, The Veterinary Research Institute, Pulawy, Poland

Bibliografia

• 1. Anczykowski F., Tereszczuk M., Hajnosz T.: Studies on the natural ’’agglutinins” in the bovine sera. I. Occurrence of the natural anti-Brucella ’’agglutinins” in sera of normal cattle. In press.
• 2. Anczykowski F.: Studies on the natural ’’agglutinins” in the bovine sera. III. Natural ’’agglutinin” level in sera of cattle vaccinated with Brucella abortus Buck 19 strain or infected with field Brucella strains. In press.
• 3. Anczykowski F., Piaseczna M., Hajnosz T.: Studies on the natural ’’agglutinins” in the bovine sera. IV. Interpopulation diversity of the natural anti-Brucella ’’agglutinin” levels. In press.
• 4. Anczykowski F.: Studies on the natural ’’agglutinins” in the bovine sera. V. Range and dynamics shiftings of the natural anti-Brucella ’’agglutinins” levels depending on ecological conditions of cattle herds. In press.
• 5. Anczykowski F., Piaseczna M.: Studies on the mercaptoethanol test specificity in the bovine brucellosis diagnosis. I. Influence of the mercaptoethanol on the agglutination activity of normal bovine sera. In press.
• 6. Kowalenko J. R., Sidorow M. A.: Arch. Exp. Vet. Med. 24, 113, 1970.
• 7. Pilet Ch., Toma B.: Rec. Med. Vet. 145, 1155, 1969.