Detekcja Phytophthora citrophthora w sztucznie zakażonych liściach przy wykorzystaniu techniki real-time PCR

• Autorzy: Trzewik A. , Orlikowska T. , Ptaszek M. , Orlikowski L. B.

Warianty tytułu

EN

Detection Phytophthora citrophthora in artificially infected leaves using realtime PCR techniques

• Języki publikacji: PL

Abstrakty

EN

Phytophthora species are considerable threat to the nurseries of trees, bushes and perennials. In Poland, several Phytophthora species were isolated from diseased plant, soil and water. P. citrophthora was isolated from deciduous and coniferous plants. With the use of real-time PCR technique with SYBR® Green and Pc2B/Pc primers and we were able to detect P. citrophthora in artificially infected rhododendron leaves with symptoms of colonization and in symptomless.

• Wydawca: -
• Czasopismo: Progress in Plant Protection
• Rocznik: 2011
• Tom: 51
• Numer: 2
• Opis fizyczny: s.695-699,rys.,bibliogr.

Twórcy

autor

Trzewik A.

autor

Orlikowska T.

autor

Ptaszek M.

autor

Orlikowski L. B.

Bibliografia

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• Ippolito A., Schena L., Nigro F., Ligorio S.V., Yassen T. 2004. Real-time detection of Phytophthora nicotianae and P. citrophthora in citrus roots and soil. Eur. J. Plant Pathol. 110: 833–843.
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• Orlikowski L.B. 2006. Relationship between source of water, used for plant sprinkling, and occurrence of Phytophthora shoot rot tip blight in container-ornamental nurseries. J. Plant Protection Res. 46 (2): 163–168.
• Orlikowski L.B., Ptaszek M., Trzewik A., Orlikowska T. 2010. The occurrence and isolate differentiation of Phytophthora spp. in polish ornamental nursery stocks. p. 230. In: First Serbian Forestry Congress ,,Future with Forests”. Belgrade, 11–13 November, 2010, 306 pp.
• Trzewik A., Wiejacha K., Orlikowski L.B., Szkuta G., Orlikowska T. 2006. The identification of five Phytophthora species on the basis of DNA markers obtained via the PCR technique with non-specific primers. Phytopathol. Pol. 41: 27–37.