Mitochondrial uncoupling protein silence is compromised in photosynthesis and redox poise

• Autorzy: Chen S. , Jin W. , Liu A.-r. , Liu Z. , Liu D. , Xia X. , Lin X.
• Języki publikacji: EN

Abstrakty

EN

Mitochondrial uncoupling proteins play important roles in generation of metabolic thermogenesis, response to stress situations, and regulation of energy metabolism. We demonstrated here that the absence of LeUCP in tomato inhibited photosynthesis using virusinduced gene-silencing approach. A significant decrease in the rate of CO2 assimilation in LeUCP-silencing plants was observed over a range of different light intensities. Absence of LeUCP resulted in lower net photosynthetic rate, lightsaturated rate of the CO2 assimilation (Asat), maximum carboxylation rates (Vcmax) and maximum RuBP regeneration rate (Jmax). Activities of ribulose-1,5-bisphosphate carboxylase/oxygenase Rubisco and stromal fructose-1,6- bisphosphatase and genes expression levels encoded Calvin cycle enzymes of LeUCP gene-silencing plants were inhibited. However, silencing of LeUCP gene had no effect on Fv/Fm, but decreased photochemical quenching and electron transport rate. Meanwhile, non-photochemical quenching and Je (PSII), the distribution of Je (PCR) and Je (PCO), the content of AsA, NAD, and the ratios of NAD?/ NADH, AsA/DHA were significantly reduced with increased reactive oxygen species while GSH and GSSG were unaltered. Taken together, these results strongly suggest that LeUCP gene in tomato leaves is crucial in maintaining the redox poise of the mitochondrial electron transport chain to facilitate photosynthetic metabolism.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 08
• Opis fizyczny: p.2547-2558,fig.,ref.

Twórcy

autor

Chen S.

• College of Forestry, Henan University of Science and Technology, Luoyang 471003, People’s Republic of China
• Department of Horticulture, Zhejiang University, Zijingang Campus, Yuhangtang Road, Hangzhou 310058, People’s Republic of China

autor

Jin W.

• College of Forestry, Henan University of Science and Technology, Luoyang 471003, People’s Republic of China

autor

Liu A.-r.

• College of Forestry, Henan University of Science and Technology, Luoyang 471003, People’s Republic of China

autor

Liu Z.

• Department of Horticulture, Zhejiang University, Zijingang Campus, Yuhangtang Road, Hangzhou 310058, People’s Republic of China

autor

Liu D.

• Key Laboratory of Horticultural Plants Growth, Development and Quality Improvement, Agricultural Ministry of China, Yuhangtang Road 866, Hangzhou 310058, People’s Republic of China

autor

Xia X.

• Department of Horticulture, Zhejiang University, Zijingang Campus, Yuhangtang Road, Hangzhou 310058, People’s Republic of China
• Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

autor

Lin X.

• College of Forestry, Henan University of Science and Technology, Luoyang 471003, People’s Republic of China

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