Persistence of ectomycorrhizas by Telephora terrestris on outplanted Scots pine seedlings

• Autorzy: Hilszczanska D. , Sierota Z.

Warianty tytułu

PL

Trwałość ektomikoryz Telephora terrestris u sadzonek sosny w uprawie

• Języki publikacji: EN

Abstrakty

EN

Thelephora terrestris(Erhr.) Fr. is a very common ectomycorrhizal symbiont (ECM) in conifer trees, however the role of this ubiquitous fungus in nurseries and Scots pine plantations is still unknown. It is described as tolerant of high nitrogen availability and therefore was taken into consideration as an important ECM partner of seedlings, particularly after replanting on post agricultural land. In laboratory the seedlings of Scots pine (Pinus sylvestris L.) were inoculated with T. terrestris(Tt/IBL/2) or not inoculated (control) and grown in containers in two different regimes of nitrogen fertilization (4g N x kg-1and 6 g N x kg-1). Next year these seedlings were outplanted in post agricultural land and 6 months later, the number and identity of some mycorrhizas were studied. It was found, that mycorrhizal abundance was higher in the inoculated treatments than in non-inoculated ones. PCR RFLP analysis confirmed share of two different isolates of Thelephoraengaged in mycorrhizal symbiosis. Part of mycorrhizas had the same pattern of RFLP as the isolate used to inoculation. Similar results were obtained in second year of experimental study in the field what confirmed the persistence of artificially introduced T. terrestrisin post agricultural soil as an important component of the ECM community.

PL

Sadzonki sosny zwyczajnej Pinus sylvestrisL., mikoryzowane grzybnią Thelephora terrestris i nie mikoryzowane (kontrola) hodowano w laboratorium w dwóch wariantach nawożenia (4g N x kg-1 and 6 g N x kg -1) a po roku wysadzono na gruncie porolnym. W ciągu dwóch sezonów wegetacyjnych badano stan ilościowy i jakościowy mikoryz oraz określono tożsamość partnera grzybowego. Wyższą ogólną liczbą mikoryz charakteryzowały się sadzonki sztucznie mikoryzowane. Analiza DNA grzybowego przeprowadzona metodą PCR RFLP wykazała, że na badanych korzeniach mikoryzy T. terrestristworzone były przez dwa izolaty Thelephora; izolat użyty do inokulacji siewek (Thelephora I) i izolat obecny w glebie (Thelephora II). Mikoryzy tworzone z udziałem inokulowanej grzybni obecne były na korzeniach jeszcze w dwa lata po wysadzeniu sadzonek na gruncie porolnym i stanowiły 30% udziału, podczas gdy mikoryzy autochtoniczne Thelephora II – 70%.

• Wydawca: -
• Czasopismo: Acta Mycologica
• Rocznik: 2006
• Tom: 41
• Numer: 2
• Opis fizyczny: p.313-318,fig.,ref.

Twórcy

autor

Hilszczanska D.

• Forest Research Institute, Bitwy Warszawskiej 1920 r. nr 3, 00-973 Warsaw, Poland

autor

Sierota Z.

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