Targeting essential genes of Nosema for the diagnosis of pebrine disease in silkworms

• Autorzy: Esvaran V. , Jagadish A. , Terenius O. , Suraporn S. , Mishra R.K. , Ponnuvel K.M.
• Języki publikacji: EN

Abstrakty

EN

Pebrine is one of the devastating diseases mostly caused by notorious Nosema – a microsporidian infecting silkworms. Identification of novel genes associated with the pathogen plays a key role in developing a reliable diagnostic tool for the detection of disease. Targeting potential biomarkers will help in developing strategies for fast and efficient control measures, which can prevent the spread of infection. This study was to identify genes present commonly in Nosema such as Nosema bombycis, Nosema mylitta, Nosema assamensis and Nosema ricini infecting Indian silkworms in order to find potential gene markers for early pebrine disease diagnosis. Real time PCR was used to validate the genes active early during the infection cycle, confirming the expression of genes and their order of expression. 16S rRNA and β-tubulin were found to be expressed early in infection followed by PTP1 and PTP2, PTP3, SWP5 and MetAP2 genes. These identified molecular markers can be used in addition to conventional gene primers which are traditionally used for the detection of pebrine.

• Wydawca: -
• Czasopismo: Annals of Parasitology
• Rocznik: 2020
• Tom: 66
• Numer: 3
• Opis fizyczny: p.303-310,fig.,ref.

Twórcy

autor

Esvaran V.

• Genomic Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bengaluru 560035, India

autor

Jagadish A.

• Genomic Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bengaluru 560035, India

autor

Terenius O.

• Department of Cell and Molecular Biology, Uppsala Universiti, Box 536, SE-751 23 Uppsala, Sweden

autor

Suraporn S.

• Department of Biology, Faculty of Science, Maha Sarakham University, Mahasarakham 45150, Thailand

autor

Mishra R.K.

• Genomic Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bengaluru 560035, India

autor

Ponnuvel K.M.

• Genomic Division, Seribiotech Research Laboratory, Carmelaram Post, Kodathi, Bengaluru 560035, India

Identyfikatory

DOI

10.17420/ap6603.268