Application of resonance Raman microscopy to in vivo carotenoid

• Autorzy: Uragami C. , Yamashita E. , Gall A. , Robert B. , Hashimoto H.
• Języki publikacji: EN

Abstrakty

EN

The high antioxidant activity of astaxanthin has been attracted considerable attention in these days. One of the major antioxidant activities of this carotenoid is anti-photoaging. We have been focusing our attention on this particular issue. The anti-photoaging activity should be functioning in inner skin. In this study we tried to find out the fact that astaxanthin that has been swabbed on the outer surface of the skin has really passed through and reached to the inner skin. For this purpose resonance Raman microscopy was applied to the rat skin sample on which astaxanthin was swabbed on its outer surface. Astaxanthin gives rise to a unique Raman spectrum that is characteristic of its molecular structure. Therefore, we can easily identify the presence or absence of astaxanthin in the area of the rat skin that is subjected to this spectroscopic measurement. We used 532 nm laser light for probing the resonance Raman scattering of astaxanthin. Astaxanthin shows three strong Raman lines at 1508, 1145, and 993 cm-1. These three lines are ascribable to the C=C stretching, C-C stretching, and C-CH3 in-plane rocking vibrational modes, respectively. We have constructed confocal Raman microscope that has the spatial resolution of ca. 500 nm. Three-dimensional mapping of the Raman spectrum of astaxanthin has been performed in order to determine its distribution in the rat skin.

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2012
• Tom: 59
• Numer: 1
• Opis fizyczny: p.53-56,fig.,ref.

Twórcy

autor

Uragami C.

• Department of Physics and CREST/JST, Graduate School of Science, Osaka City University, Sugimoto, Sumiyoshi-ku, Osaka, Japan

autor

Yamashita E.

autor

Gall A.

autor

Robert B.

autor

Hashimoto H.

Bibliografia

• Caspers PJ, Lucassen GW, Carter EA, Bruining HA, Puppels GJ (2001) In vivo confocal raman microspectroscopy of the skin: noninvasive determination of molecular concentration profiles. J Invest Dermatol 116: 434-442. 
• Darvin ME, Gersonde I, Albrecht H, Sterry W, Landemann J (2006) Non-Invasive in vivo detection of the carotenoid antioxidant substance lycopene in the human skin using the resonance Raman spectroscopy. Laser Phys Lett 3: 460-463.
• Dieing T, Hollricher O, Toporski J (2010) Confocal Raman Microscopy, Pronger Series in Optical Sciences 158, Springer-Verlag, Berlin, Heidelberg, Germany.
• Hussein G, Sankawa U, Goto H, Matsumoto K, Watanabe H (2006) Astaxanthin, a carotenoid with potential human health and nutrition. J Nat Prod 69: 443-449. 
• Ichihashi M, Ando H, Yoshida M, Niki Y, Matsui M (2009) Photoaging of the skin. Anti-Aging Med 6: 46-59.
• Kurihara H, Koda H, Asami S, Kiso Y, Tanaka T (2002) Contribution of the antioxidative property of astaxanthin to its protective effect on the promotion of cancer metastasis in mice treated with restraint stress. Life Sci 70: 2509-2520. 
• Landrum JT (2010) Carotenoids: Physical, Chemical and Biological Functions and Properties, CRS Press, Florida, USA.
• Merlin JC (1985) Resonance Raman spectroscopy of carotenoids and carotenoid-containing systems. Pure Appl Chem 57: 785-792.
• Nijssen A, Bakker Schut TC, Heule F, Caspres PJ, Hayes DP, Neumann MH, Puppels GJ (2002) Discriminating basal cell carcinoma from its surrounding tissue by Raman spectroscopy. J Invest Dermatol 119: 64-69. 
• Saito S, Tasumi M (1983) Normal-coordinate analysis of β-carotene isomers and assignments of the Raman and infrared bands. J Raman Spectroscopy 14: 310-321.
• Watanabe H, Hussein G, Goto H, Nakagawa T, Oda S, Matsumoto K, Sankawa U (2007) Astaxanthin improves metabolic syndrome in the rat model. Carotenoid Sci 11: 84-89.

Uwagi

PL

Rekord w opracowaniu.