Effects of nitrogen source and phosphate concentration on biomass and metabolites accumulation in adventitious root culture of Glycyrrhiza uralensis Fisch

• Autorzy: Yin S. , Zhang Y. , Gao W. , Wang J. , Man S. , Liu H.
• Języki publikacji: EN

Abstrakty

EN

We investigated the influence of ammonia/ nitrate ratio and phosphate concentration on biomass and accumulation of metabolites in adventitious roots of Glycyrrhiza uralensis Fisch. A NH₄⁺/NO₃⁻ ratio of 10:20 was optimal for the production of biomass (0.30 g dry weight) as well as polysaccharide (18.98 mg g⁻¹) and glycyrrhetinic acid (0.31 mg g⁻¹). The content of glycyrrhizic acid (0.47 mg g⁻¹) and flavonoid (8.11 mg g⁻¹) reached the optimum at an ammonia/nitrate ratio of 15:15 and 20:10, respectively. In case of phosphate concentration, a higher growth rate (9.18) and content of polysaccharide (15.66 mg g⁻¹) was obtained at 1.25 mM phosphate concentration. However, 0.625 mM phosphate was favorable for the content of flavonoid (7.54 mg g⁻¹) and glycyrrhizic acid (0.57 mg g⁻¹). The content of glycyrrhetinic acid (0.32 mg g⁻¹) reached the peak when treated with 0.3125 mM phosphate. A scale-up culture of adventitious roots was established using a balloon-type bubble bioreactor (BTBB). Maximum growth rates of 25.95- and 22.44-fold were obtained in 3 and 5 L BTBBs, respectively, which was higher than that in 0.5 L shake flask (18.36). The contents of flavonoid (7.60 mg g⁻¹) and polysaccharide (15.09 mg g⁻¹) reached the peak in 5 L and 3 L BTBB, respectively. The contents of glycyrrhizic and glycyrrhetinic acid were a little higher than that in BTBBs.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 04
• Opis fizyczny: p.915-921,fig.,ref.

Twórcy

autor

Yin S.

• Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, 300072 Tianjin, China

autor

Zhang Y.

• Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, 300072 Tianjin, China

autor

Gao W.

• Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, 300072 Tianjin, China

autor

Wang J.

• Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, 300072 Tianjin, China

autor

Man S.

• Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Univrsity of Science and Technology, 300457 Tianjin, China

autor

Liu H.

• Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin Univrsity of Science and Technology, 300457 Tianjin, China

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Identyfikatory

DOI

10.1007/s11738-013-1470-z