Application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for identification of coagulase-negative staphylococci isolated from milk of cows with subclinical mastitis

• Autorzy: Banach T. , Bocniarz M. , Lyp P. , Adaszek L. , Wawron W. , Furmaga B. , Skrzypczak M. , Zietek J. , Winiarczyk S.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to use matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification of coagulase-negative staphylococci (CNS) isolated from the milk of cows with subclinical mastitis. The study material consisted of 33 isolates of CNS, identified by the results of API Staph tests, obtained from the milk of cows with subclinical mastitis. Based on the spectra analyses, MALDI-TOF MS tests of 33 bacterial samples allowed identification of the microorganisms in 27 cases (81.8%). The most frequent cause of subclinical mastitis was found to be Staphylococcus sciuri (39%), while S. vitulinus was detected in 15% of the milk samples. The results obtained indicate that MALDI-TOF MS can be used for the identification of CNS isolated from bovine mastitis as a method supplementary to biochemical tests.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2016
• Tom: 19
• Numer: 3
• Opis fizyczny: p.627-632,fig.,ref.

Twórcy

autor

Banach T.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612 Lublin, Poland

autor

Bocniarz M.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-950 Lublin, Poland

autor

Lyp P.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612 Lublin, Poland

autor

Adaszek L.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612 Lublin, Poland

autor

Wawron W.

• Department and Clinic of Animal Reproduction, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-950 Lublin, Poland

autor

Furmaga B.

• Sub-Department of Toxicology and Environmental Protection, Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences, Akademicka 12, 20-950 Lublin, Poland

autor

Skrzypczak M.

• Second Department of Gynecology, Prof. F. Skubiszewski University School of Medicine, Jaczewskiego 8, 20-090 Lublin, Poland

autor

Zietek J.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612 Lublin, Poland

autor

Winiarczyk S.

• Department of Epizootiology and Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences, Gleboka 30, 20-612 Lublin, Poland

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Identyfikatory

DOI

10.1515/pjvs-2016-0078