Biochemical responses of glyphosate resistant and susceptible soybean plants exposed to glyphosate

• Autorzy: Moldes C.A. , Medici L. O. , Abrahao O. S. , Tsai S. M. , Azevedo R. A.
• Języki publikacji: EN

Abstrakty

EN

Glyphosate is a wide spectrum, non-selective, post-emergence herbicide. It acts on the shikimic acid pathway inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), thus obstructing the synthesis of tryptophan, phenylalanine, tyrosine and other secondary products, leading to plant death. Transgenic glyphosateresistant (GR) soybean [Glycine max (L.)] expressing an glyphosate-insensitive EPSPS enzyme has provided new opportunities for weed control in soybean production. The effect of glyphosate application on chlorophyll level, lipid peroxidation, catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GOPX) and superoxide dismutase (SOD) activities, soluble amino acid levels and protein profile, in leaves and roots, was examined in two conventional (non-GR) and two transgenic (GR) soybean. Glyphosate treatment had no significant impact on lipid peroxidation, whilst the chlorophyll content decreased in only one non-GR cultivar. However, there was a significant increase in the levels of soluble amino acid in roots and leaves, more so in non-GR than in GR soybean cultivars. Root CAT activity increased in non-GR cultivars and was not altered in GR cultivars. In leaves, CAT activity was inhibited in one non-GR and one GR cultivar. GOPX activity increased in one GR cultivar and in both non-GR cultivars. Root APX activity increased in one GR cultivar. The soluble protein profiles as assessed by 1-D gel electrophoresis of selected non-GR and GR soybean lines were unaffected by glyphosate treatment. Neither was formation of new isoenzymes of SOD and CAT observed when these lines were treated by glyphosate. The slight oxidative stress generated by glyphosate has no relevance to plant mortality. The potential antioxidant action of soluble amino acids may be responsible for the lack of lipid peroxidation observed. CAT activity in the roots and soluble amino acids in the leaves can be used as indicators of glyphosate resistance.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 4
• Opis fizyczny: p.469-479,fig.,ref.

Twórcy

autor

Moldes C.A.

• Facultad de Ciencias Exactas y Naturales, Universidad Nacional de La Pampa, 6300 Santa Rosa, Argentina

autor

Medici L. O.

• Departamento de Ciencias Fisiologicas, Universidade Federal Rural do Rio de Janeiro, Seropedica, 23890-000 RJ CEP, Brazil

autor

Abrahao O. S.

• Centro de Energia Nuclear na Agricultura, Univrsidade de Sao Paulo, Piracicaba, 13400-970 SP, Brazil

autor

Tsai S. M.

• Centro de Energia Nuclear na Agricultura, Univrsidade de Sao Paulo, Piracicaba, 13400-970 SP, Brazil

autor

Azevedo R. A.

• Escola Superior de Agricultura "Luiz de Queiroz", Universidade de Sao Paulo, Piracicaba, 13418-900 SP CEP, Brazil

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