Rola węgla aktywowanego i benzyloaminopuryny w mikrorozmnażaniu obuwika pospolitego

• Autorzy: Pindel A.

Warianty tytułu

EN

The role of activated charcoal and benzylaminopurine in micropropagation of Cypripedium calceolus L.

• Języki publikacji: PL

Abstrakty

PL

Badano zdolność morfogenetyczną eksplantatów pochodzących z niedojrzałych torebek nasiennych obuwika pospolitego (Cypripedium calceolus L.) na pożywkach o zróżnicowanej zawartości benzyloaminopuryny (BA) i węgla aktywowanego (AC). Powstawanie protokormo-podobnych struktur (PLB) obserwowano na pożywce MS z 1 mg·dm⁻³ BA i 0,1% AC. Ten początkowy etap morfogenezy trwał aż 12 miesięcy, obserwacje anatomiczne wykazały jednak, że został zainicjowany etap różnicowania komórek, co daje nadzieję na regenerację pędów. Wykazano jednocześnie, że brak w podłożu AC prowadzi do ciemnienia eksplantatów i pożywki co w konsekwencji skutkowało zamieraniem kultury.

EN

The morphogenetic ability of Cypripedium calceolus L. explants from green capsules on the medium with different additions of benzylaminopurine (BA) and activated charcoal (CA) were investigated. Protocorm-like bodies were observed on the MS medium with 1 mg·dm⁻³ BA and 0.1% AC after 12 months. The anatomical study showed that the cells are competent to caulogenesis. It was presented heat on the media without charcoal the explants turned brown and died.

• Wydawca: -
• Czasopismo: Zeszyty Problemowe Postępów Nauk Rolniczych
• Rocznik: 2007
• Tom: 523
• Opis fizyczny: s.163-168,tab.,fot.,bibliogr.

Twórcy

autor

Pindel A.

Bibliografia

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